CUL3 cullin 3
Model Species
Mus musculusGene Aliases
KIAA0617Models / Rescue Models
17 / 5Rescue Model
-Model Summary
Cul3 null mice are embryonic lethal and the abnormalies start at E6.5. Cells in extraembryonic ectoderm and ectoplacental cone exhibit prolonged S phase in cell cycle, which supports that Cul3 might regulate the turnover of Cycline E. Cul3 hets have abnormal cardiovescular function, renal function, and hyperkalaemia.
External Links
Reports related to CUL3 (5 Reports)
# | Type | Title | Author, Year |
---|---|---|---|
1 | - | Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. | Singer JD , et al. (1999) |
2 | - | Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia. | Schumacher FR , et al. (2015) |
3 | Additional | Behavioral, circuitry, and molecular aberrations by region-specific deficiency of the high-risk autism gene Cul3. | Rapanelli M , et al. (2019) |
4 | Additional | CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance Through the Promotion of Cap-Dependent Translation </title> | () |
5 | Additional | Autism-linked Cullin3 germline haploinsufficiency impacts cytoskeletal dynamics and cortical neurogenesis through RhoA signaling | Amar M et al. (2021) |
M_CUL3_10_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mice with selective deletion of Cul3 exons 4 to 7 in the pyramidal neurons of the dorsal telencephalon, neocortex, and hippocampus were generated by crossing conditional ready Cul3 mice (Jackson Laboratory stock no 028349) with heterozygous Nex Cre transgenic mice where Cre expression begins at embryonic day 11.5.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory; Dr. Klaus-Armin Nave, Max Planck Institute (Goebbels et al., 2006, PMID 17146780 ) (Nex-Cre)
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | P60-p80 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neocortex morphology | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Hippocampal morphology | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Increased | Golgi-cox staining | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | No change | Golgi-cox staining | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic length | No change | Golgi-cox staining | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | No change | Measurement of tissue weight | P14 | () |
Neurophysiology | Miniature post synaptic current frequency: inhibitory | Increased | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current frequency: excitatory | Increased | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current amplitude: inhibitory | No change | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell current clamp | P60 | () |
Neurophysiology | Membrane potential | No change | Whole-cell current clamp | P60 | () |
Neurophysiology | Epsp-spike relationship | Increased | Whole-cell current clamp | P60 | () |
Repetitive behavior | NA | Not reported | NA | NA | () |
Seizure | NA | Not reported | NA | NA | () |
Sensory | NA | Not reported | NA | NA | () |
Social behavior | Social memory | Decreased | Three-chamber social approach test | P60-p80 | () |
Social behavior | Social approach | Decreased | Three-chamber social approach test | P60-p80 | () |
Physiological parameters | NA | Not reported | NA | NA | () |
Communications | NA | Not reported | NA | NA | () |
Developmental profile | Mortality/lethality | No change | Kaplan-meier survival curve | P0-p40 | () |
Emotion | Anxiety | Increased | Open field test | P60-p80 | () |
Emotion | Anxiety | Increased | Elevated plus maze test | P60-p80 | () |
Immune response | NA | Not reported | NA | NA | () |
Learning & memory | NA | Not reported | NA | NA | () |
Maternal behavior | NA | Not reported | NA | NA | () |
Molecular profile | Targeted expression | Decreased | Western blot | P14 | () |
M_CUL3_10_CKO_HT_VHIPP_INACT
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Activity of ventral hippocampal (vHPC) pyramidal cells of adult Nex-Cre Cul3 het CKO mutant mice was reduced by a DREADD (designer receptors exclusively activated by designer drugs) chemical genetic approach where mice were bilaterally injected with the virus AAV1-DIO-hM4Di:mCherry, which expressed hM4Di in a Cre-dependent manner followed by 2 mg/kg intraperitoneal clozapine-N-oxide injection to inhibit neurons by Gi-dependent activation of inward potassium channels. Controls were injected with the control virus, AAV1-DIO-mCherry.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory; Dr. Klaus-Armin Nave, Max Planck Institute (Goebbels et al., 2006, PMID 17146780 ) (Nex-Cre)
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
Motor phenotype | General locomotor activity: ambulatory activity | No adverse effect | Open field test | P60 | () |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | () |
Neurophysiology | Membrane potential | Side effect | Whole-cell current clamp | P60 | () |
Neurophysiology | Epsp-spike relationship | Restored | Whole-cell current clamp | P60 | () |
Repetitive behavior | NA | Not reported | NA | NA | () |
Seizure | NA | Not reported | NA | NA | () |
Sensory | NA | Not reported | NA | NA | () |
Social behavior | NA | Not reported | NA | NA | () |
Physiological parameters | NA | Not reported | NA | NA | () |
Communications | NA | Not reported | NA | NA | () |
Developmental profile | NA | Not reported | NA | NA | () |
Emotion | Anxiety | Restored | Elevated plus maze test | P60 | () |
Emotion | Anxiety | Restored | Open field test | P60 | () |
Immune response | NA | Not reported | NA | NA | () |
Learning & memory | NA | Not reported | NA | NA | () |
Maternal behavior | NA | Not reported | NA | NA | () |
Molecular profile | NA | Not reported | NA | NA | () |
M_CUL3_11_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with selective deletion of Cul3 exons 4 to 7 in the pyramidal neurons of the dorsal telencephalon, neocortex, and hippocampus were generated by crossing conditional ready Cul3 mice (Jackson Laboratory stock no 028349) with heterozygous Nex Cre transgenic mice where Cre expression begins at embryonic day 11.5.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory; Dr. Klaus-Armin Nave, Max Planck Institute (Goebbels et al., 2006, PMID 17146780 ) (Nex-Cre)
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
Motor phenotype | NA | Not reported | NA | NA | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neocortex morphology | Abnormal | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Hippocampal morphology | Abnormal | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | Decreased | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Decreased | Measurement of tissue weight | P14 | () |
Neurophysiology | NA | Not reported | NA | NA | () |
Repetitive behavior | NA | Not reported | NA | NA | () |
Seizure | NA | Not reported | NA | NA | () |
Sensory | NA | Not reported | NA | NA | () |
Social behavior | NA | Not reported | NA | NA | () |
Physiological parameters | NA | Not reported | NA | NA | () |
Communications | NA | Not reported | NA | NA | () |
Developmental profile | Mortality/lethality | Increased | Kaplan-meier survival curve | P0-p21 | () |
Emotion | NA | Not reported | NA | NA | () |
Immune response | NA | Not reported | NA | NA | () |
Learning & memory | NA | Not reported | NA | NA | () |
Maternal behavior | NA | Not reported | NA | NA | () |
Molecular profile | Targeted expression | Decreased | Western blot | P14 | () |
M_CUL3_12_KO_HT
Model Type: Genetic LOF
Model Genotype: Heterozygous
Mutation:
Happloinsufficient mouse line generated using crispr/cas9 genome editing to introduce a 1-bp insertion in exon 6 at the locus of an asd mutation e246x (p.glu246stop).
Allele Type: Knockout
Strain of Origin: C57BL/6N
Genetic Background: C57BL/6N
Es cell line: C57BL/6N
Model Source: Amar M, et al, Mol Psych, 2021
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Motor phenotype | Righting response | Decreased | Developmental milestone measurements | P0-21 | Amar M et al. (2021) |
Motor phenotype | Motor coordination and balance | Decreased | Cliff avoidance test | P0-21 | Amar M et al. (2021) |
Motor phenotype | Hyperactivity | Increased | Open field test | Adult | Amar M et al. (2021) |
Motor phenotype | General locomotor activity: Ambulatory activity | Increased | Open field test | Adult | Amar M et al. (2021) |
Motor phenotype | General locomotor activity | Increased | Open field test | Adult | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Olfactory bulb morphology | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | Decreased | Immunohistochemistry | 8-10 weeks | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | Decreased | Cell counting | E14 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal morphology | No change | Immunohistochemistry | E17.5 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neocortex morphology: size | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the insular cortex | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Hypothalamic morphology | Increased | Magnetic resonance imaging (MRI) | P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Hippocampal morphology | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | Decreased | Immunohistochemistry | E17.5 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic length | Decreased | Immunohistochemistry | E17.5 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | Decreased | Immunohistochemistry | E14 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cerebellar morphology | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cell proliferation: neural precursors | No change | Immunohistochemistry | - | Amar M et al. (2021) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Decreased | Magnetic resonance imaging (MRI) | 8-10 weeks, P7 | Amar M et al. (2021) |
Neurophysiology | Neuronal apoptosis | Increased | Immunohistochemistry | 8-10 weeks | Amar M et al. (2021) |
Neurophysiology | Network excitability | Decreased | Multielectrode array recording | E17.5 | Amar M et al. (2021) |
Neurophysiology | Intrinsic bursting events or spikes | Decreased | Multielectrode array recording | E17.5 | Amar M et al. (2021) |
Repetitive behavior | Self grooming | No change | Grooming behavior assessments | Adult | Amar M et al. (2021) |
Social behavior | Social memory | Decreased | Three-chamber social approach test | Adult | Amar M et al. (2021) |
Social behavior | Social approach | No change | Three-chamber social approach test | Adult | Amar M et al. (2021) |
Physiological parameters | Reproductive function | No change | General observations | Adult | Amar M et al. (2021) |
Developmental profile | Size/growth | Decreased | Body weight measurement | E15.5, E17.5, Adult | Amar M et al. (2021) |
Developmental profile | Mortality/lethality | No change | General observations | Adult | Amar M et al. (2021) |
Developmental profile | Developmental trajectory | No change | Developmental milestone measurements | P0-21 | Amar M et al. (2021) |
Emotion | Anxiety | No change | Open field test | Adult | Amar M et al. (2021) |
Learning & memory | Object recognition memory | Decreased | Novel object recognition test | Adult | Amar M et al. (2021) |
Molecular profile | Targeted expression | Decreased | Quantitative PCR (qRT-PCR) | Adult | Amar M et al. (2021) |
Molecular profile | Targeted expression | Decreased | Western blot | Adult | Amar M et al. (2021) |
Molecular profile | Proteomic profile diversity | Abnormal | Tandem mass tagging (TMT) | E17.5, P7, 4-6 weeks | Amar M et al. (2021) |
Molecular profile | Protein expression level evidence | Increased | Tandem mass tagging (TMT) | E17.5, P7, 4-6 weeks | Amar M et al. (2021) |
Molecular profile | Protein expression level evidence | Increased | Western blot | E17.5, Adult | Amar M et al. (2021) |
Molecular profile | Differential gene expression | Abnormal | RNA sequencing | E17.5, P7, 4-6 weeks | Amar M et al. (2021) |
Molecular profile | Cytoskeletal organization | Decreased | Immunohistochemistry | E17.5 | Amar M et al. (2021) |
Molecular profile | Cytoskeletal organization | Decreased | Western blot | Adult | Amar M et al. (2021) |
Molecular profile | Cytoskeletal organization | Decreased | RNA sequencing | E17.5, P7, 4-6 weeks | Amar M et al. (2021) |
Molecular profile | Cytoskeletal organization | Decreased | Tandem mass tagging (TMT) | E17.5, P7, 4-6 weeks | Amar M et al. (2021) |
M_CUL3_12_KO_HT_RHOSIN
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Happloinsufficient mouse line generated using crispr/cas9 genome editing to introduce a 1-bp insertion in exon 6 at the locus of an asd mutation e246x (p.glu246stop).
Allele Type: Knockout
Strain of Origin: C57BL/6N
Genetic Background: C57BL/6N
Es cell line: C57BL/6N
Model Source: Amar M, et al, Mol Psych, 2021
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic length | Restored | Immunohistochemistry | E17.5 | Amar M et al. (2021) |
Neurophysiology | Network excitability | Restored | Multielectrode array recording | E17.5 | Amar M et al. (2021) |
Neurophysiology | Intrinsic bursting events or spikes | Restored | Multielectrode array recording | E17.5 | Amar M et al. (2021) |
M_CUL3_2_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Exon 9 of Cullin3, flanked by flippase-recognition target (FRT) sites, was removed by Flp recombinase, which resulted in Cul3 (amino acids 403-459) truncated product.
Allele Type: Targeted (knockin)
Strain of Origin: C57BL/6N
Genetic Background: C57BL/6J
Es cell line: Not specified
Model Source: Taconic
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Motor phenotype | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Neurophysiology | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Repetitive behavior | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Seizure | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Sensory | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Social behavior | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Physiological parameters | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Communications | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Developmental profile | Mortality/lethality | Increased | General observations | P1 | Schumacher FR , et al. (2015) |
Emotion | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Immune response | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Learning & memory | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Maternal behavior | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Molecular profile | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
M_CUL3_2_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Exon 9 of Cullin3, flanked by flippase-recognition target (FRT) sites, was removed by Flp recombinase, which resulted in Cul3 (amino acids 403-459) truncated product.
Allele Type: Targeted (knockin)
Strain of Origin: C57BL/6N
Genetic Background: C57BL/6J
Es cell line: Not specified
Model Source: Taconic
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Motor phenotype | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Neurophysiology | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Repetitive behavior | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Seizure | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Sensory | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Social behavior | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Physiological parameters | Renal function | Decreased | Urine/blood electrolyte analysis | Unreported | Schumacher FR , et al. (2015) |
Physiological parameters | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Physiological parameters | Muscle fiber contraction | Increased | In vivo vasoconstrictor response assay | Unreported | Schumacher FR , et al. (2015) |
Physiological parameters | Hormone levels | Increased | Blood chemistry | Unreported | Schumacher FR , et al. (2015) |
Communications | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Developmental profile | Size/growth | Decreased | Voxel based morphometry | Unreported | Schumacher FR , et al. (2015) |
Developmental profile | Cardiovascular development and function | Decreased | Voxel based morphometry | Unreported | Schumacher FR , et al. (2015) |
Developmental profile | Cardiovascular development and function | Decreased | Blood pressure measurement | Unreported | Schumacher FR , et al. (2015) |
Emotion | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Immune response | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Learning & memory | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Maternal behavior | NA | Not reported | NA | NA | Schumacher FR , et al. (2015) |
Molecular profile | Targeted expression | Decreased | Western blot | Unreported | Schumacher FR , et al. (2015) |
Molecular profile | Signaling | Increased | Western blot | Unreported | Schumacher FR , et al. (2015) |
Molecular profile | Protein expression: in situ protein expression | Abnormal | Immunostaining | Unreported | Schumacher FR , et al. (2015) |
Molecular profile | Protein expression: in situ protein expression | No change | Immunostaining | Unreported | Schumacher FR , et al. (2015) |
Molecular profile | Protein expression level evidence | No change | Western blot | Unreported | Schumacher FR , et al. (2015) |
Molecular profile | Protein expression level evidence | Increased | Immunostaining | Unreported | Schumacher FR , et al. (2015) |
M_CUL3_3_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Exon sequence corresponding to amino acids 127-293 of Cul3 was deleted and replaced with neomycin gene driven by the PGK promoter, which resulted in a null allele.
Allele Type: Targeted (knockout)
Strain of Origin: Not specified
Genetic Background: C57/B6J; 129/Sv
Es cell line: 129/Sv
Model Source: Not specified
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Motor phenotype | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Neurophysiology | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Repetitive behavior | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Seizure | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Sensory | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Social behavior | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Physiological parameters | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Communications | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Developmental profile | Size/growth | Decreased | Immunostaining | E6.5-e7.5 | Singer JD , et al. (1999) |
Developmental profile | Mortality/lethality: embryonic | Increased | General observations | P1 | Singer JD , et al. (1999) |
Emotion | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Immune response | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Learning & memory | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Maternal behavior | NA | Not reported | NA | NA | Singer JD , et al. (1999) |
Molecular profile | Protein expression: in situ protein expression | Increased | Immunostaining | E7.5 | Singer JD , et al. (1999) |
Molecular profile | Cell proliferation | Abnormal | Immunostaining | E7.5 | Singer JD , et al. (1999) |
M_CUL3_4_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Cul3^flox/fox mice from Jackson Laboratory (stock no.: 028349) with exons 4 to 7 flanked by loxp sites were bred with Emx1-IRES-Cre knockin mice (stock no.: 005628) to generate forebrain-specific Cul3 heterozygous knockout mice (Emx1Cre/+; Cul3^f/+ or Cul3^f/) converting a hypomorphic allele to a null allele after Cre recombination. Emx1 directs Cul3 KO in cortical excitatory glutamatergic neurons, but not inhibitory GABAergic interneurons, and other forebrain structures, including the hippocampus and amygdala.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Motor phenotype | Motor learning | No change | Accelerating rotarod test | 6 weeks | Rapanelli M , et al. (2019) |
Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 6 weeks | Rapanelli M , et al. (2019) |
Motor phenotype | General locomotor activity: ambulatory activity | Increased | Open field test | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine morphology | Decreased | Golgi-cox staining | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-cox staining | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | No change | Golgi-cox staining | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic length | No change | Golgi-cox staining | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cytoskeletal organization: neuronal | No change | Immunostaining | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Ameliorated | 16s rrna gene sequence analysis for identification of bacteria | P6 | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | No change | Gross necroscopy | P6 | Rapanelli M , et al. (2019) |
Neurophysiology | Spontaneous post synaptic event frequency: excitatory currents | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Presynaptic function: paired-pulse depression (ppd) | Decreased | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Membrane potential | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Epsp-spike relationship | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Decay kinetics of miniature post synaptic currents | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 6 weeks | Rapanelli M , et al. (2019) |
Seizure | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Sensory | Sensorimotor gating | Decreased | Prepulse inhibition | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social memory | Decreased | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social approach | Decreased | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Physiological parameters | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Communications | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Developmental profile | Size/growth | No change | Body weight measurement | P6, 5 weeks | Rapanelli M , et al. (2019) |
Developmental profile | Mortality/lethality | No change | General observations | 6-7 weeks | Rapanelli M , et al. (2019) |
Emotion | Anxiety | No change | Open field test | 6 weeks | Rapanelli M , et al. (2019) |
Immune response | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Learning & memory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Maternal behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Molecular profile | Targeted expression | Decreased | Immunostaining | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Targeted expression | Decreased | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Protein expression level evidence | No change | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Protein expression level evidence | Increased | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Protein expression level evidence | Abnormal | Liquid chromatography-mass spectrometry (lc-ms) | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Gene expression | No change | Semi-quantitative pcr (qrt-pcr) | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Dna methylation | Increased | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
M_CUL3_4_CKO_HT_BCI-121
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Mice with forebrain specific deletion of Cul3 were systemically administered BCI-121 (1 mg/kg, i.p., once daily for 3 days).
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Motor phenotype | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neurophysiology | Synaptic transmission: excitatory | Restored | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Presynaptic function: paired-pulse depression (ppd) | Refractory | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Repetitive behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Seizure | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Sensory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Social behavior | Social memory | Restored | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social approach | Restored | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Physiological parameters | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Communications | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Developmental profile | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Emotion | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Immune response | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Learning & memory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Maternal behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Molecular profile | Dna methylation | Restored | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
M_CUL3_4_CKO_HT_SMYD3-KD
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Mice with forebrain specific deletion of Cul3 were injected with lentiviral particles containing a shRNA sequence against Smyd3 to knock down Smyd3 expression in the PFC. Controls were injected with a scrambled control shRNA.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Motor phenotype | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neurophysiology | Presynaptic function: paired-pulse facilitation | No adverse effect | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Epsp-spike relationship | Restored | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Repetitive behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Seizure | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Sensory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Social behavior | Social memory | Restored | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social approach | Restored | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Physiological parameters | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Communications | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Developmental profile | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Emotion | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Immune response | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Learning & memory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Maternal behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Molecular profile | Gene expression | Side effect | Semi-quantitative pcr (qrt-pcr) | 6 weeks | Rapanelli M , et al. (2019) |
M_CUL3_5_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Cul3^flox/fox mice from Jackson Laboratory (stock no.: 028349) with exons 4 to 7 flanked by loxp sites were bred with Emx1-IRES-Cre knockin mice (stock no.: 005628) to generate forebrain-specific Cul3 homozygous knockout mice, converting a hypomorphic allele to a null allele after Cre recombination. Emx1 directs Cul3 KO in cortical excitatory glutamatergic neurons, but not inhibitory GABAergic interneurons, and other forebrain structures, including the hippocampus and amygdala.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Motor phenotype | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Decreased | Gross necroscopy | P6 | Rapanelli M , et al. (2019) |
Neurophysiology | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Repetitive behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Seizure | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Sensory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Social behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Physiological parameters | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Communications | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Developmental profile | Size/growth | No change | Body weight measurement | 5 weeks | Rapanelli M , et al. (2019) |
Developmental profile | Size/growth | Decreased | Body weight measurement | P6 | Rapanelli M , et al. (2019) |
Developmental profile | Mortality/lethality | Increased | General observations | P7 | Rapanelli M , et al. (2019) |
Emotion | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Immune response | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Learning & memory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Maternal behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Molecular profile | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
M_CUL3_6_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Cul3^flox/fox mice were crossed with either AAV8-CaMKIIalpha-GFPCre or AAV8-CaMKIIalpha-GFP (control) in the PFC.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Motor phenotype | Motor learning | No change | Accelerating rotarod test | 6 weeks | Rapanelli M , et al. (2019) |
Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 6 weeks | Rapanelli M , et al. (2019) |
Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neurophysiology | Synaptic transmission: excitatory | Decreased | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Spontaneous post synaptic event frequency: excitatory currents | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Membrane potential | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Epsp-spike relationship | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 6 weeks | Rapanelli M , et al. (2019) |
Seizure | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6 weeks | Rapanelli M , et al. (2019) |
Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social memory | Decreased | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social approach | Decreased | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Physiological parameters | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Communications | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Developmental profile | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Emotion | Anxiety | No change | Open field test | 6 weeks | Rapanelli M , et al. (2019) |
Immune response | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Learning & memory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Maternal behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Molecular profile | Targeted expression | Decreased | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Targeted expression | Decreased | Immunostaining | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Protein expression level evidence | Abnormal | Liquid chromatography-mass spectrometry (lc-ms) | 6 weeks | Rapanelli M , et al. (2019) |
M_CUL3_7_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Cul3^flox/fox mice were stereotaxically injected bilaterally with either AAV8-CaMKIIa-GFPCre or AAV8-CaMKIIa-GFP (control) in the dorsal striatum.
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Motor phenotype | Motor learning | No change | Accelerating rotarod test | 6 weeks | Rapanelli M , et al. (2019) |
Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 6 weeks | Rapanelli M , et al. (2019) |
Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6 weeks | Rapanelli M , et al. (2019) |
Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Neurophysiology | Spontaneous post synaptic event amplitude: inhibitory currents | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Neuronal activation | Decreased | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Miniature post synaptic current frequency: inhibitory | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Miniature post synaptic current amplitude: inhibitory | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Neurophysiology | Action potential property: firing rate | Decreased | Whole-cell patch clamp | 6 weeks | Rapanelli M , et al. (2019) |
Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 6 weeks | Rapanelli M , et al. (2019) |
Seizure | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6 weeks | Rapanelli M , et al. (2019) |
Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6 weeks | Rapanelli M , et al. (2019) |
Social behavior | Social approach | No change | Three-chamber social approach test | 6 weeks | Rapanelli M , et al. (2019) |
Physiological parameters | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Communications | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Developmental profile | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Emotion | Anxiety | No change | Open field test | 6 weeks | Rapanelli M , et al. (2019) |
Immune response | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Learning & memory | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Maternal behavior | NA | Not reported | NA | NA | Rapanelli M , et al. (2019) |
Molecular profile | Targeted expression | Decreased | Immunostaining | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Targeted expression | Decreased | Western blot | 6 weeks | Rapanelli M , et al. (2019) |
Molecular profile | Protein expression level evidence | Abnormal | Liquid chromatography-mass spectrometry (lc-ms) | 6 weeks | Rapanelli M , et al. (2019) |
M_CUL3_8_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mice with selective deletion of Cul3 exons 4 to 7 in the central nervous system, including astrocytes, oligodendroglia, ependyma and some neurons were generated by crossing conditional ready Cul3 mice (Jackson Laboratory stock no 028349) with GFAP Cre transgenic mice (Jackson Laboratory stock no 004600).
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
Motor phenotype | Motor learning | No change | Accelerating rotarod test | P60-p80 | () |
Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | P60-p80 | () |
Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | P60-p80 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Size of cerebral ventricles | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size: inhibitory synapses | No change | Immunohistochemistry | P14-17 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | No change | Immunohistochemistry | P60 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Hippocampal morphology | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Increased | Golgi-cox staining | P60, p14-p17 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | No change | Golgi-cox staining | P60 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic length | No change | Golgi-cox staining | P60 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical lamination | No change | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | No change | Measurement of tissue weight | P14 | () |
Neurophysiology | Presynaptic function: paired-pulse facilitation | Decreased | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current frequency: inhibitory | Increased | Whole-cell current clamp | P60, p15 | () |
Neurophysiology | Miniature post synaptic current frequency: excitatory | Increased | Whole-cell current clamp | P60, p15 | () |
Neurophysiology | Miniature post synaptic current amplitude: inhibitory | No change | Whole-cell current clamp | P60, p15 | () |
Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell current clamp | P60, p15 | () |
Neurophysiology | Membrane potential | No change | Whole-cell current clamp | P60, p15 | () |
Neurophysiology | Epsp-spike relationship | Increased | Whole-cell current clamp | P60, p15 | () |
Neurophysiology | Decay kinetics of miniature post synaptic currents | Increased | Whole-cell current clamp | P60 | () |
Neurophysiology | Action potential property: firing rate | Increased | Whole-cell current clamp | P60 | () |
Repetitive behavior | Stereotypy | No change | Y-maze test | P60-p80 | () |
Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | P60-p80 | () |
Seizure | NA | Not reported | NA | NA | () |
Sensory | Olfaction | No change | Buried food test | P60-p80 | () |
Social behavior | Social memory | Decreased | Three-chamber social approach test | P60-p80 | () |
Social behavior | Social approach | Decreased | Three-chamber social approach test | P60-p80 | () |
Physiological parameters | Reproductive function | No change | Kaplan-meier survival curve | P0-p40 | () |
Physiological parameters | NA | Not reported | NA | NA | () |
Communications | NA | Not reported | NA | NA | () |
Developmental profile | Size/growth | No change | Body weight measurement | P14 | () |
Developmental profile | Mortality/lethality | No change | Kaplan-meier survival curve | P0-p40 | () |
Emotion | Anxiety | Increased | Open field test | P60-p80 | () |
Emotion | Anxiety | Increased | Elevated plus maze test | P60-p80 | () |
Immune response | NA | Not reported | NA | NA | () |
Learning & memory | Spatial working memory | No change | Y-maze test | P60-p80 | () |
Maternal behavior | NA | Not reported | NA | NA | () |
Molecular profile | Targeted expression | Decreased | Western blot | P14 | () |
Molecular profile | Regulation of translation | Abnormal | Western blot | P60 | () |
Molecular profile | Protein ubiquitination | Decreased | Western blot | P14 | () |
Molecular profile | Protein expression level evidence | Increased | Western blot | P60 | () |
Molecular profile | Protein expression level evidence | Decreased | Western blot | P60 | () |
Molecular profile | Protein expression level evidence | Abnormal | Tandem mass tagging (tmt) | P60 | () |
Molecular profile | Protein binding | Increased | Western blot | P14 | () |
M_CUL3_8_CKO_HT_4EGI-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Cap-dependent translation was inhibitted in GFAP-Cre Cul3 CKO het mice by intra cerebralventricular (i.c.v.) injection (50mM, bilateral, 0.5 mL daily, for 12 days) of 4EGI-1, an inhibitor of the eIF4E-eIF4G1 complex (Fan et al., 2010).
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
Motor phenotype | General locomotor activity: ambulatory activity | No adverse effect | Open field test | P60 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Restored | Golgi-cox staining | P60 | () |
Neurophysiology | Presynaptic function: paired-pulse facilitation | Ameliorated | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current frequency: inhibitory | Refractory | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current frequency: excitatory | Restored | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current amplitude: inhibitory | No adverse effect | Whole-cell current clamp | P60 | () |
Neurophysiology | Miniature post synaptic current amplitude: excitatory | No adverse effect | Whole-cell current clamp | P60 | () |
Neurophysiology | Membrane potential | No adverse effect | Whole-cell current clamp | P60 | () |
Neurophysiology | Epsp-spike relationship | Refractory | Whole-cell current clamp | P60 | () |
Neurophysiology | Decay kinetics of miniature post synaptic currents | Ameliorated | Whole-cell current clamp | P60 | () |
Neurophysiology | Action potential property: firing rate | Restored | Whole-cell current clamp | P60 | () |
Repetitive behavior | NA | Not reported | NA | NA | () |
Seizure | NA | Not reported | NA | NA | () |
Sensory | NA | Not reported | NA | NA | () |
Social behavior | Social memory | Restored | Three-chamber social approach test | P60-p80 | () |
Social behavior | Social approach | Restored | Three-chamber social approach test | P60-p80 | () |
Physiological parameters | NA | Not reported | NA | NA | () |
Communications | NA | Not reported | NA | NA | () |
Developmental profile | NA | Not reported | NA | NA | () |
Emotion | Anxiety | Refractory | Elevated plus maze test | P60 | () |
Emotion | Anxiety | Refractory | Open field test | P60 | () |
Immune response | NA | Not reported | NA | NA | () |
Learning & memory | NA | Not reported | NA | NA | () |
Maternal behavior | NA | Not reported | NA | NA | () |
Molecular profile | NA | Not reported | NA | NA | () |
M_CUL3_9_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with selective deletion of Cul3 exons 4 to 7 in the central nervous system, including astrocytes, oligodendroglia, ependyma and some neurons were generated by crossing conditional ready Cul3 mice (Jackson Laboratory stock no 028349) with heterozygous GFAP Cre transgenic mice (Jackson Laboratory stock no 004600).
Allele Type: Conditonal knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson laboratory
Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
---|---|---|---|---|---|
Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
Motor phenotype | NA | Not reported | NA | NA | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Size of cerebral ventricles | Increased | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Hippocampal morphology | Abnormal | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | Decreased | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical lamination | Abnormal | Histology | P14 | () |
Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Decreased | Measurement of tissue weight | P14 | () |
Neurophysiology | NA | Not reported | NA | NA | () |
Repetitive behavior | NA | Not reported | NA | NA | () |
Seizure | NA | Not reported | NA | NA | () |
Sensory | NA | Not reported | NA | NA | () |
Social behavior | NA | Not reported | NA | NA | () |
Physiological parameters | NA | Not reported | NA | NA | () |
Communications | NA | Not reported | NA | NA | () |
Developmental profile | Size/growth | Decreased | Body weight measurement | P14 | () |
Developmental profile | Mortality/lethality | Increased | Kaplan-meier survival curve | P0-p20 | () |
Emotion | NA | Not reported | NA | NA | () |
Immune response | NA | Not reported | NA | NA | () |
Learning & memory | NA | Not reported | NA | NA | () |
Maternal behavior | NA | Not reported | NA | NA | () |
Molecular profile | Targeted expression | Decreased | Western blot | P14 | () |
Molecular profile | Regulation of translation | Abnormal | Western blot | P14 | () |
Molecular profile | Protein expression level evidence | Abnormal | Tandem mass tagging (tmt) | P14 | () |
Other CUL3 Rescue Models(5 Models)
M_CUL3_4_CKO_HT | 1 | Genetic | Pharmaceutical | BCI-121 |
M_CUL3_4_CKO_HT | 1 | Genetic | Genetic | Smyd3 knockdown |
M_CUL3_10_CKO_HT | 1 | Genetic | Genetic | Chemogenetic reduction in activity of ventral hippocampal pyramidal neurons |
M_CUL3_8_CKO_HT | 1 | Genetic | Pharmaceutical | 4EGI-1 |
M_CUL3_12_KO_HT | 1 | Genetic LOF | Pharmaceutical | Rhosin |
CNVs associated with CUL3(1 Models)
Sort By:
2q36.2 | 5 | Deletion | 7 / 13 |