Genetic Animal Models / LRFN2

LRFN2 leucine rich repeat and fibronectin type III domain containing 2

Model Species
Mus musculus
Gene Aliases
5730420O05Rik, SALM1, mKIAA1246
Reports
1
Associated Human Genes
LRFN2
Models / Rescue Models
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Rescue Model
-
Model Summary

Lrfn2 homozygous null mice exhibit decreased body weight, social interaction, social approach, mating-induced USV, expression of the presynaptic protein PSD-95, spine density in the pyramidal neurons of the hippocampal CA1 region, length of the postsynaptic density, mEPSC frequency, GluA1 in the postsynaptic density and increase social withdrawal, number of silent synapses compared to wildtype controls.

Reports related to LRFN2 (1 Reports)
# Type Title Author, Year
1 Primary Autism-like behaviours and enhanced memory formation and synaptic plasticity in Lrfn2/SALM1-deficient mice. Morimura N , et al. (2017)
Show all Construct Details Show all Phenotypic Profiles

M_LRFN2_1_KO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Mice with exon 2 of Lrfn2 replaced by a neo cassette. The neo cassette was removed by germline Cre recombination and heterozygous knockouts were backcrossed to C57BL/6J for six generations.

Allele Type: Knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J

Es cell line: -
Model Source: 28604739
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters Reproductive function No change General observations Adult Morimura N , et al. (2017)
Circadian sleep/wake cycle - Not Reported - - Morimura N , et al. (2017)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test 2-6 months Morimura N , et al. (2017)
Motor phenotype Hyperactivity Increased Running wheel test 2-6 months Morimura N , et al. (2017)
Motor phenotype General locomotor activity: Ambulatory activity No change Home cage behavior 2-6 months Morimura N , et al. (2017)
Motor phenotype General locomotor activity No change Home cage behavior 2-6 months Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic morphology: synaptic cleft length Increased Electron microscopy 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic morphology: active zone No change Western blot 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic morphology: active zone Decreased Western blot 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic morphology: active zone Decreased Quantitative PCR (qRT-PCR) 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic morphology: active zone Decreased Immunohistochemistry 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic morphology Abnormal Electron microscopy 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Post-synaptic density size Decreased Electron microscopy 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: NMDA receptors No change Western blot 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: NMDA receptors No change Quantitative PCR (qRT-PCR) 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: NMDA receptors Increased Western blot 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: NMDA receptors Increased Quantitative PCR (qRT-PCR) 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: AMPA receptors No change Western blot 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: Inhibitory neurons No change Immunohistochemistry 5-8 month Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: Excitatory neurons No change Immunohistochemistry 5-8 month Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine morphology Abnormal Golgi-Cox staining 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Golgi-Cox staining 8 week Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture Brain anatomy No change Histology: cresyl violet Adult Morimura N , et al. (2017)
Neurophysiology Synaptic transmission Decreased Whole-cell patch clamp 8-14 weeks Morimura N , et al. (2017)
Neurophysiology Synaptic plasticity: hippocampal LTP Increased Long term potentiation (LTP) 8-14 weeks Morimura N , et al. (2017)
Neurophysiology Synaptic neuroreceptor ratio (NMDAR/AMPAR) dependent transmission Increased Whole-cell patch clamp 8-14 weeks Morimura N , et al. (2017)
Neurophysiology Neurotransmitter release No change Paired-pulse ratio 8-14 weeks Morimura N , et al. (2017)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp 8-14 weeks Morimura N , et al. (2017)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp 8-14 weeks Morimura N , et al. (2017)
Neurophysiology Decay kinetics of evoked post synaptic currents Decreased Whole-cell patch clamp 8-14 weeks Morimura N , et al. (2017)
Repetitive behavior Repetitive digging No change Reciprocal social interaction test 2-6 months Morimura N , et al. (2017)
Repetitive behavior Perseveration Increased Marble-burying test 2-6 months Morimura N , et al. (2017)
Repetitive behavior Perseveration Increased Running wheel test 2-6 months Morimura N , et al. (2017)
Seizure - Not Reported - - Morimura N , et al. (2017)
Sensory Vision No change Morris water maze test 2-6 months Morimura N , et al. (2017)
Sensory Startle response: acoustic stimulus Increased Acoustic startle reflex test 2-6 months Morimura N , et al. (2017)
Sensory Sensorimotor gating Increased Prepulse inhibition 2-6 months Morimura N , et al. (2017)
Sensory Pain or nociception No change Foot shock test 2-6 months Morimura N , et al. (2017)
Sensory Olfaction No change Response to olfactory stimuli 2-6 months Morimura N , et al. (2017)
Social behavior Social withdrawal Increased Reciprocal social interaction test 2-6 months Morimura N , et al. (2017)
Social behavior Social interaction Decreased Reciprocal social interaction test 2-6 months Morimura N , et al. (2017)
Social behavior Social approach Decreased Three-chamber social approach test 2-6 months Morimura N , et al. (2017)
Communications Ultrasonic vocalization: Isolation induced No change Monitoring ultrasonic vocalizations 0-3weeks Morimura N , et al. (2017)
Communications Ultrasonic vocalization: Interaction induced: opposite sex stimulus Decreased Monitoring ultrasonic vocalizations 2-6 months Morimura N , et al. (2017)
Developmental profile Size/growth Decreased Body weight measurement 6-10 weeks Morimura N , et al. (2017)
Developmental profile Mortality/lethality No change Genotypic ratio of progeny from heterozygous parents 3-8 weeks Morimura N , et al. (2017)
Emotion Anxiety No change Light-dark exploration test: Light-dark exploration test 2-6 months Morimura N , et al. (2017)
Emotion Anxiety No change Open field test 2-6 months Morimura N , et al. (2017)
Emotion Anxiety No change Elevated plus maze test 2-6 months Morimura N , et al. (2017)
Homeostasis - Not Reported - - Morimura N , et al. (2017)
Immune response - Not Reported - - Morimura N , et al. (2017)
Learning & memory Spatial working memory No change Morris water maze test 2-6 months Morimura N , et al. (2017)
Learning & memory Spatial reference memory Increased Morris water maze test 2-6 months Morimura N , et al. (2017)
Learning & memory Cued or contextual fear conditioning: Memory of cue No change Fear conditioning test 2-6 months Morimura N , et al. (2017)
Learning & memory Cued or contextual fear conditioning: Memory of context: Long term recall Increased Fear conditioning test 2-6 months Morimura N , et al. (2017)
Learning & memory Cued or contextual fear conditioning: Memory of context Increased Fear conditioning test 2-6 months Morimura N , et al. (2017)
Maternal behavior - Not Reported - - Morimura N , et al. (2017)
Molecular profile Targeted expression Decreased Western blot Adult Morimura N , et al. (2017)
Molecular profile Protein localization: synapse Decreased Western blot: fractionation - Morimura N , et al. (2017)
Molecular profile Protein localization: synapse No change Western blot: fractionation - Morimura N , et al. (2017)

M_LRFN2_2_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Mice with exon 2 of Lrfn2 replaced by a neo cassette. The neo cassette was removed by germline Cre recombination and heterozygous knockouts were backcrossed to C57BL/6J for six generations.

Allele Type: Knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J

Es cell line: -
Model Source: 28604739
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Circadian sleep/wake cycle - Not Reported - - Morimura N , et al. (2017)
Motor phenotype - Not Reported - - Morimura N , et al. (2017)
Neuroanatomy / Ultrastructure / Cytoarchitecture - Not Reported - - Morimura N , et al. (2017)
Neurophysiology - Not Reported - - Morimura N , et al. (2017)
Repetitive behavior - Not Reported - - Morimura N , et al. (2017)
Seizure - Not Reported - - Morimura N , et al. (2017)
Sensory - Not Reported - - Morimura N , et al. (2017)
Social behavior - Not Reported - - Morimura N , et al. (2017)
Communications - Not Reported - - Morimura N , et al. (2017)
Developmental profile - Not Reported - - Morimura N , et al. (2017)
Emotion - Not Reported - - Morimura N , et al. (2017)
Homeostasis - Not Reported - - Morimura N , et al. (2017)
Immune response - Not Reported - - Morimura N , et al. (2017)
Learning & memory - Not Reported - - Morimura N , et al. (2017)
Maternal behavior - Not Reported - - Morimura N , et al. (2017)
Molecular profile Targeted expression Decreased Western blot Adult Morimura N , et al. (2017)
CNVs associated with LRFN2(1 Models)
6p21.2-p21.1 1 Deletion 1  /  1
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