LRFN2 leucine rich repeat and fibronectin type III domain containing 2
Model Species
Mus musculusGene Aliases
5730420O05Rik, SALM1, mKIAA1246Models / Rescue Models
-Rescue Model
-Model Summary
Lrfn2 homozygous null mice exhibit decreased body weight, social interaction, social approach, mating-induced USV, expression of the presynaptic protein PSD-95, spine density in the pyramidal neurons of the hippocampal CA1 region, length of the postsynaptic density, mEPSC frequency, GluA1 in the postsynaptic density and increase social withdrawal, number of silent synapses compared to wildtype controls.
External Links
Reports related to LRFN2 (1 Reports)
| # | Type | Title | Author, Year |
|---|---|---|---|
| 1 | Primary | Autism-like behaviours and enhanced memory formation and synaptic plasticity in Lrfn2/SALM1-deficient mice. | Morimura N , et al. (2017) |
Show all Construct Details
Show all Phenotypic Profiles
M_LRFN2_1_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with exon 2 of Lrfn2 replaced by a neo cassette. The neo cassette was removed by germline Cre recombination and heterozygous knockouts were backcrossed to C57BL/6J for six generations.
Allele Type: Knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: 28604739
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 2-6 months | Morimura N , et al. (2017) |
| Motor phenotype | Hyperactivity | Increased | Running wheel test | 2-6 months | Morimura N , et al. (2017) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Home cage behavior | 2-6 months | Morimura N , et al. (2017) |
| Motor phenotype | General locomotor activity | No change | Home cage behavior | 2-6 months | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology: synaptic cleft length | Increased | Electron microscopy | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology: active zone | No change | Western blot | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology: active zone | Decreased | Western blot | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology: active zone | Decreased | Quantitative pcr (qrt-pcr) | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology: active zone | Decreased | Immunohistochemistry | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology | Abnormal | Electron microscopy | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size | Decreased | Electron microscopy | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Quantitative pcr (qrt-pcr) | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Increased | Western blot | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Increased | Quantitative pcr (qrt-pcr) | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: inhibitory neurons | No change | Immunohistochemistry | 5-8 month | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: excitatory neurons | No change | Immunohistochemistry | 5-8 month | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine morphology | Abnormal | Golgi-cox staining | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-cox staining | 8 week | Morimura N , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain anatomy | No change | Histology | Adult | Morimura N , et al. (2017) |
| Neurophysiology | Synaptic transmission | Decreased | Whole-cell patch clamp | 8-14 weeks | Morimura N , et al. (2017) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Increased | Long term potentiation (ltp) | 8-14 weeks | Morimura N , et al. (2017) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Increased | Whole-cell patch clamp | 8-14 weeks | Morimura N , et al. (2017) |
| Neurophysiology | Neurotransmitter release | No change | Paired-pulse ratio | 8-14 weeks | Morimura N , et al. (2017) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | 8-14 weeks | Morimura N , et al. (2017) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 8-14 weeks | Morimura N , et al. (2017) |
| Neurophysiology | Decay kinetics of evoked post synaptic currents | Decreased | Whole-cell patch clamp | 8-14 weeks | Morimura N , et al. (2017) |
| Repetitive behavior | Repetitive digging | No change | Reciprocal social interaction test | 2-6 months | Morimura N , et al. (2017) |
| Repetitive behavior | Perseveration | Increased | Running wheel test | 2-6 months | Morimura N , et al. (2017) |
| Repetitive behavior | Perseveration | Increased | Marble-burying test | 2-6 months | Morimura N , et al. (2017) |
| Seizure | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Sensory | Vision | No change | Morris water maze test | 2-6 months | Morimura N , et al. (2017) |
| Sensory | Startle response: acoustic stimulus | Increased | Acoustic startle reflex test | 2-6 months | Morimura N , et al. (2017) |
| Sensory | Sensorimotor gating | Increased | Prepulse inhibition | 2-6 months | Morimura N , et al. (2017) |
| Sensory | Pain or nociception | No change | Foot shock test | 2-6 months | Morimura N , et al. (2017) |
| Sensory | Olfaction | No change | Response to olfactory stimuli | 2-6 months | Morimura N , et al. (2017) |
| Social behavior | Social withdrawal | Increased | Reciprocal social interaction test | 2-6 months | Morimura N , et al. (2017) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 2-6 months | Morimura N , et al. (2017) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 2-6 months | Morimura N , et al. (2017) |
| Physiological parameters | Reproductive function | No change | General observations | Adult | Morimura N , et al. (2017) |
| Physiological parameters | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | 0-3weeks | Morimura N , et al. (2017) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Decreased | Monitoring ultrasonic vocalizations | 2-6 months | Morimura N , et al. (2017) |
| Developmental profile | Size/growth | Decreased | Body weight measurement | 6-10 weeks | Morimura N , et al. (2017) |
| Developmental profile | Mortality/lethality | No change | Genotypic ratio of progeny from heterozygous parents | 3-8 weeks | Morimura N , et al. (2017) |
| Emotion | Anxiety | No change | Open field test | 2-6 months | Morimura N , et al. (2017) |
| Emotion | Anxiety | No change | Light-dark exploration test | 2-6 months | Morimura N , et al. (2017) |
| Emotion | Anxiety | No change | Elevated plus maze test | 2-6 months | Morimura N , et al. (2017) |
| Immune response | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Learning & memory | Spatial working memory | No change | Morris water maze test | 2-6 months | Morimura N , et al. (2017) |
| Learning & memory | Spatial reference memory | Increased | Morris water maze test | 2-6 months | Morimura N , et al. (2017) |
| Learning & memory | Cued or contextual fear conditioning: memory of cue | No change | Fear conditioning test | 2-6 months | Morimura N , et al. (2017) |
| Learning & memory | Cued or contextual fear conditioning: memory of context: long term recall | Increased | Fear conditioning test | 2-6 months | Morimura N , et al. (2017) |
| Learning & memory | Cued or contextual fear conditioning: memory of context | Increased | Fear conditioning test | 2-6 months | Morimura N , et al. (2017) |
| Maternal behavior | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Molecular profile | Targeted expression | Decreased | Western blot | Adult | Morimura N , et al. (2017) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | NA | Morimura N , et al. (2017) |
| Molecular profile | Protein localization: synapse | No change | Western blot | NA | Morimura N , et al. (2017) |
M_LRFN2_2_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mice with exon 2 of Lrfn2 replaced by a neo cassette. The neo cassette was removed by germline Cre recombination and heterozygous knockouts were backcrossed to C57BL/6J for six generations.
Allele Type: Knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: -
Model Source: 28604739
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Motor phenotype | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Neurophysiology | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Repetitive behavior | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Seizure | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Sensory | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Social behavior | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Physiological parameters | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Communications | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Developmental profile | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Emotion | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Immune response | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Learning & memory | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Maternal behavior | NA | Not reported | NA | NA | Morimura N , et al. (2017) |
| Molecular profile | Targeted expression | Decreased | Western blot | Adult | Morimura N , et al. (2017) |
CNVs associated with LRFN2(1 Models)
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| 6p21.2-p21.1 | 1 | Deletion | 1 / 1 |