Genetic Mouse Models / SHANK3

SHANK3 SH3 and multiple ankyrin repeat domains 3

Model Species
Mus musculus
Gene Aliases
OTTMUSP00000028933; SH3 and multiple ankyrin repeat domains protein 3; SPANK-2; proSAP2; proline-rich synapse-associated protein 2
Reports
33
Associated Human Genes
SHANK3
Models / Rescue Models
95 / 37
Rescue Model
-
Model Summary

Specific deficits in synaptic function and plasticity, along with reduced reciprocal social interactions in Shank3 heterozygous mice.

Reports related to SHANK3 (33 Reports)
# Type Title Author, Year
1 Primary Haploinsufficiency of the autism-associated Shank3 gene leads to deficits in synaptic function, social interaction, and social communication. Bozdagi O , et al. (2010)
2 Additional Shank3 mutant mice display autistic-like behaviours and striatal dysfunction. Pea J , et al. (2011)
3 Additional Synaptic dysfunction and abnormal behaviors in mice lacking major isoforms of Shank3. Wang X , et al. (2011)
4 Additional Enhanced polyubiquitination of Shank3 and NMDA receptor in a mouse model of autism. Bangash MA , et al. (2011)
5 Additional Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2. Schmeisser MJ , et al. (2012)
6 Additional Insulin-like growth factor-1 rescues synaptic and motor deficits in a mouse model of autism and developmental delay. Bozdagi O , et al. (2013)
7 Additional Loss of predominant Shank3 isoforms results in hippocampus-dependent impairments in behavior and synaptic transmission. Kouser M , et al. (2013)
8 Additional Autism-like Deficits in Shank3-Deficient Mice Are Rescued by Targeting Actin Regulators. Duffney LJ , et al. (2015)
9 Additional Autism-Associated Insertion Mutation (InsG) of Shank3 Exon 21 Causes Impaired Synaptic Transmission and Behavioral Deficits. Speed HE , et al. (2015)
10 Additional Cerebellar associative sensory learning defects in five mouse autism models. Kloth AD , et al. (2015)
11 Additional Altered Striatal Synaptic Function and Abnormal Behaviour in Shank3 Exon4-9 Deletion Mouse Model of Autism. Jaramillo TC , et al. (2015)
12 Additional Mice with Shank3 Mutations Associated with ASD and Schizophrenia Display Both Shared and Distinct Defects. Zhou Y , et al. (2015)
13 Additional CLK2 inhibition ameliorates autistic features associated with SHANK3 deficiency. Bidinosti M , et al. (2016)
14 Additional Adult restoration of Shank3 expression rescues selective autistic-like phenotypes. Mei Y , et al. (2016)
15 Additional Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. Wang X , et al. (2016)
16 Additional Peripheral Mechanosensory Neuron Dysfunction Underlies Tactile and Behavioral Deficits in Mouse Models of ASDs. Orefice LL , et al. (2016)
17 Additional Novel Shank3 mutant exhibits behaviors with face validity for autism and altered striatal and hippocampal function. Jaramillo TC , et al. (2016)
18 Additional Comprehensive analysis of two Shank3 and the Cacna1c mouse models of autism spectrum disorder. Kabitzke PA , et al. (2017)
19 Additional Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition. Qin L , et al. (2018)
20 Additional Dysbiosis of microbiome and probiotic treatment in a genetic model of autism spectrum disorders. Tabouy L , et al. (2018)
21 Additional Heterogeneity of Cell Surface Glutamate and GABA Receptor Expression in Shank and CNTN4 Autism Mouse Models. Heise C , et al. (2018)
22 Additional Dietary Zinc Supplementation Prevents Autism Related Behaviors and Striatal Synaptic Dysfunction in Shank3 Exon 13-16 Mutant Mice. Fourie C , et al. (2018)
23 Additional Mechanisms Underlying Microbial-Mediated Changes in Social Behavior in Mouse Models of Autism Spectrum Disorder. Sgritta M , et al. (2018)
24 Additional An autism-linked missense mutation in SHANK3 reveals the modularity of Shank3 function. Wang L , et al. (2019)
25 Additional Striatal Inhibition of MeCP2 or TSC1 Produces Sociability Deficits and Repetitive Behaviors. Lee Y , et al. (2019)
26 Additional Hypothesis-driven investigations of diverse pharmacological targets in two mouse models of autism. Rhine MA , et al. (2019)
27 Additional Shank3 modulates sleep and expression of circadian transcription factors. Ingiosi AM , et al. (2019)
28 Additional Anterior cingulate cortex dysfunction underlies social deficits in Shank3 mutant mice. Guo B , et al. (2019)
29 Additional Targeting Peripheral Somatosensory Neurons to Improve Tactile-Related Phenotypes in ASD Models. Orefice LL , et al. (2019)
30 Additional IL-17a Promotes Sociability in Mouse Models of Neurodevelopmental Disorders </title> ()
31 Additional Tau Reduction Prevents Key Features of Autism in Mouse Models Tai C et al. (2020)
32 Additional Autism-Associated Shank3 Is Essential for Homeostatic Compensation in Rodent V1 Tatavarty V et al. (2020)
33 Additional Autism-associated SHANK3 mutations impair maturation of neuromuscular junctions and striated muscles Lutz AK et al. (2020)
Show all Construct Details Show all Phenotypic Profiles

M_SHANK3_10_KI_HM_INSG

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Autism-associated guanine nucleotide insertion mutation (Shank3G) present in exon 21 was inserted into the Shank 3 gene, with the construct containing the mutation within exon21 sequence knocked into intron 20 .The additional G results in a frameshift mutation and a premature stop codon downstream of exon 21. Mice containing the Shank3G mutation were crossed for 4 generations with C57BL6J. Het and homozygous mice were generated as littermates.

Allele Type: ASD LOF mutation
Strain of Origin: 129Sv/Evs6
Genetic Background: C57BL/6J*129Sv/Evs6

Es cell line: SM-1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Speed HE , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Speed HE , et al. (2015)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test 2-6 months Speed HE , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic neuroreceptors No change Western blot 5-6 months Speed HE , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot 5-6 months Speed HE , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot 5-6 months Speed HE , et al. (2015)
Neurophysiology Synaptic transmission Decreased Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic plasticity: post-tetanic potentiation (ptp) Decreased Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltp No change Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltd Decreased Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Decreased Whole-cell patch clamp NA Speed HE , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp NA Speed HE , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp NA Speed HE , et al. (2015)
Neurophysiology Decay kinetics of evoked post synaptic currents No change Whole-cell patch clamp NA Speed HE , et al. (2015)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments 2-6 months Speed HE , et al. (2015)
Repetitive behavior Repetitive digging Decreased Marble-burying test 2-6 months Speed HE , et al. (2015)
Seizure NA Not reported NA NA Speed HE , et al. (2015)
Sensory Vision No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Social behavior Social memory No change Three-chamber social approach test 2-6 months Speed HE , et al. (2015)
Social behavior Social approach No change Three-chamber social approach test 2-6 months Speed HE , et al. (2015)
Social behavior Nest building behavior Decreased Nest building assay 2-6 months Speed HE , et al. (2015)
Communications NA Not reported NA NA Speed HE , et al. (2015)
Developmental profile Size/growth No change Body weight measurement NA Speed HE , et al. (2015)
Emotion Thigmotaxis No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Emotion Response to novelty Decreased Open field test 5-6 months Speed HE , et al. (2015)
Emotion Response to novelty Decreased Light-dark exploration test 5-6 months Speed HE , et al. (2015)
Emotion Response to novelty Decreased Elevated plus maze test 5-6 months Speed HE , et al. (2015)
Emotion Anxiety No change Open field test 5-6 months Speed HE , et al. (2015)
Emotion Anxiety No change Light-dark exploration test 5-6 months Speed HE , et al. (2015)
Emotion Anxiety No change Elevated plus maze test 5-6 months Speed HE , et al. (2015)
Immune response NA Not reported NA NA Speed HE , et al. (2015)
Learning & memory Spatial reference memory No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Learning & memory Spatial learning Decreased Morris water maze test 2-6 months Speed HE , et al. (2015)
Maternal behavior NA Not reported NA NA Speed HE , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 5-6 months Speed HE , et al. (2015)
Molecular profile Protein phosphorylation No change Western blot 5-6 months Speed HE , et al. (2015)
Molecular profile Protein phosphorylation Decreased Western blot 5-6 months Speed HE , et al. (2015)

M_SHANK3_10_KI_HM_INSG_REINSTATE-SHANK3

Model Type: RESCUE-Genetic
Model Genotype: Homozygous

Mutation:

Genetic reinstatement of wild type Shank3 as the Shank3G cassette containing knockin mice generated in the previous steps were crossed with CreESRT transgenic mice (B6.Cg-Tg(CAG-cre/Esr1)5Amc/J, activated by Tamoxifen administration to the mice, to excise the insertion reversing the mutation.

Allele Type: ASD LOF mutation
Strain of Origin: 129Sv/Evs6
Genetic Background: C57BL/6J*129Sv/Evs6*B6

Es cell line: SM-1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Speed HE , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Speed HE , et al. (2015)
Motor phenotype NA Not reported NA NA Speed HE , et al. (2015)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Speed HE , et al. (2015)
Neurophysiology NA Not reported NA NA Speed HE , et al. (2015)
Repetitive behavior NA Not reported NA NA Speed HE , et al. (2015)
Seizure NA Not reported NA NA Speed HE , et al. (2015)
Sensory NA Not reported NA NA Speed HE , et al. (2015)
Social behavior NA Not reported NA NA Speed HE , et al. (2015)
Communications NA Not reported NA NA Speed HE , et al. (2015)
Developmental profile NA Not reported NA NA Speed HE , et al. (2015)
Emotion NA Not reported NA NA Speed HE , et al. (2015)
Immune response NA Not reported NA NA Speed HE , et al. (2015)
Learning & memory NA Not reported NA NA Speed HE , et al. (2015)
Maternal behavior NA Not reported NA NA Speed HE , et al. (2015)
Molecular profile Targeted expression Restored Western blot 3 months Speed HE , et al. (2015)

M_SHANK3_10_KI_HT_INSG

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Autism-associated guanine nucleotide insertion mutation (Shank3G) present in exon 21 was inserted into the Shank 3 gene, with the construct containing the mutation within exon21 sequence knocked into intron 20 .The additional G results in a frameshift mutation and a premature stop codon downstream of exon 21. Mice containing the Shank3G mutation were crossed for 4 generations with C57BL6J. Het and homozygous mice were generated as littermates.

Allele Type: ASD LOF mutation
Strain of Origin: 129Sv/Evs6
Genetic Background: C57BL/6J*129Sv/Evs6

Es cell line: SM-1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Speed HE , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Speed HE , et al. (2015)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test 2-6 months Speed HE , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Synaptic neuroreceptors No change Western blot 5-6 months Speed HE , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot 5-6 months Speed HE , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot 5-6 months Speed HE , et al. (2015)
Neurophysiology Synaptic transmission Decreased Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltp No change Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltd Decreased Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp NA Speed HE , et al. (2015)
Neurophysiology Presynaptic function: presynaptic fiber volley No change Field potential recordings NA Speed HE , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp NA Speed HE , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp NA Speed HE , et al. (2015)
Neurophysiology Decay kinetics of evoked post synaptic currents No change Whole-cell patch clamp NA Speed HE , et al. (2015)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments 2-6 months Speed HE , et al. (2015)
Repetitive behavior Repetitive digging No change Marble-burying test 2-6 months Speed HE , et al. (2015)
Seizure NA Not reported NA NA Speed HE , et al. (2015)
Sensory Vision No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Social behavior Social memory No change Three-chamber social approach test 2-6 months Speed HE , et al. (2015)
Social behavior Social approach No change Three-chamber social approach test 2-6 months Speed HE , et al. (2015)
Social behavior Nest building behavior Decreased Nest building assay 2-6 months Speed HE , et al. (2015)
Communications NA Not reported NA NA Speed HE , et al. (2015)
Developmental profile Size/growth No change Body weight measurement NA Speed HE , et al. (2015)
Emotion Thigmotaxis No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Emotion Response to novelty No change Open field test 5-6 months Speed HE , et al. (2015)
Emotion Response to novelty No change Light-dark exploration test 5-6 months Speed HE , et al. (2015)
Emotion Response to novelty No change Elevated plus maze test 5-6 months Speed HE , et al. (2015)
Emotion Anxiety No change Open field test 5-6 months Speed HE , et al. (2015)
Emotion Anxiety No change Light-dark exploration test 5-6 months Speed HE , et al. (2015)
Emotion Anxiety No change Elevated plus maze test 5-6 months Speed HE , et al. (2015)
Immune response NA Not reported NA NA Speed HE , et al. (2015)
Learning & memory Spatial reference memory No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Learning & memory Spatial learning No change Morris water maze test 2-6 months Speed HE , et al. (2015)
Maternal behavior NA Not reported NA NA Speed HE , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 5-6 months Speed HE , et al. (2015)
Molecular profile Protein phosphorylation No change Western blot 5-6 months Speed HE , et al. (2015)

M_SHANK3_13_KO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Targeted Cre-LoxP mediated excision of exons 4-9 of Shank3 gene containing the ankyrin repeats. After germline transmission in C57BL6J, knock in mice were mated with mice expressing Flp1 recombinase to excise the Neo cassette used for positive selection. The resulting conditional Shank3 mice were crossed with mice expressing ZP3-Cre to excise exon4-9 in oocytes of female pups, then further crossed with C57BL6J, to homozygosity (Authors do not note that the construct used or the final mice are the same as that reported used in Bozdagi et al Mol Autism (2010) or Yang et al J Neurosci (2012), even if exon 4-9 are targeted in all these studies, therefore they are presumed to be different here).

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Physiological parameters NA Not reported NA NA Jaramillo TC , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Jaramillo TC , et al. (2015)
Motor phenotype NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test NA Jaramillo TC , et al. (2015)
Motor phenotype General locomotor activity No change Open field test NA Jaramillo TC , et al. (2015)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Orefice LL , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Jaramillo TC , et al. (2015)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Neurophysiology Synaptic transmission: excitatory No change Field potential recordings 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltp Decreased Field potential recordings 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltd No change Field potential recordings 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Decreased Whole-cell patch clamp 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Paired-pulse ratio P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp 3-4 weeks Jaramillo TC , et al. (2015)
Repetitive behavior NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments 3-5 months Jaramillo TC , et al. (2015)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Jaramillo TC , et al. (2015)
Sensory Tactile memory No change Textured novel object recognition test (t-nort) 6 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Response to air puff 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue No change Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue No change Response to air puff 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Sensory Vision No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 3-5 months Jaramillo TC , et al. (2015)
Sensory Sensorimotor gating No change Prepulse inhibition 3-5 months Jaramillo TC , et al. (2015)
Social behavior NA Not reported NA NA Orefice LL , et al. (2019)
Social behavior Social memory No change Reciprocal social interaction test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social interaction: with juveniles No change Reciprocal social interaction test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social interaction Decreased Reciprocal social interaction test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social interaction No change Partition test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Nest building behavior No change Nest building assay 3-5 months Jaramillo TC , et al. (2015)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Communications Ultrasonic vocalization: isolation induced Increased Monitoring ultrasonic vocalizations P4, p6 Jaramillo TC , et al. (2015)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Jaramillo TC , et al. (2015)
Emotion NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Thigmotaxis Increased Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Open field test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Marble-burying test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Light-dark exploration test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Elevated plus maze test 3-5 months Jaramillo TC , et al. (2015)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Jaramillo TC , et al. (2015)
Learning & memory Object recognition memory: long-term recall Decreased Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Learning & memory Object recognition memory No change Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Learning & memory Spatial reference memory No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Spatial reference memory Decreased Object-place recognition test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Spatial learning No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Object recognition memory Decreased Object-place recognition test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Cued or contextual fear conditioning No change Fear conditioning test NA Jaramillo TC , et al. (2015)
Learning & memory Cognitive flexibility No change Morris water maze test NA Jaramillo TC , et al. (2015)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Jaramillo TC , et al. (2015)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile Targeted expression Decreased Western blot 3-6 months Jaramillo TC , et al. (2015)
Molecular profile Protein localization: synapse No change Western blot 3-6 months Jaramillo TC , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot 3-6 months Jaramillo TC , et al. (2015)

M_SHANK3_13_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Targeted Cre-LoxP mediated excision of exons 4-9 of Shank3 gene containing the ankyrin repeats. After germline transmission in C57BL6J, knock in mice were mated with mice expressing Flp1 recombinase to excise the Neo cassette used for positive selection. The resulting conditional Shank3 mice were crossed with mice expressing ZP3-Cre to excise exon4-9 in oocytes of female pups, then further crossed with C57BL6J, to homozygosity (Authors do not note that the construct used or the final mice are the same as that reported used in Bozdagi et al Mol Autism (2010) or Yang et al J Neurosci (2012), even if exon 4-9 are targeted in all these studies, therefore they are presumed to be different here).

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Jaramillo TC , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Jaramillo TC , et al. (2015)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test 3-5 months Jaramillo TC , et al. (2015)
Motor phenotype General locomotor activity No change Open field test 3-5 months Jaramillo TC , et al. (2015)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Jaramillo TC , et al. (2015)
Neurophysiology Synaptic transmission: excitatory No change Field potential recordings 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltp No change Field potential recordings 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Synaptic plasticity: hippocampal ltd No change Field potential recordings 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Decreased Whole-cell patch clamp 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Paired-pulse ratio 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp 3-4 weeks Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp P14-p17 Jaramillo TC , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp 3-4 weeks Jaramillo TC , et al. (2015)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments 3-5 months Jaramillo TC , et al. (2015)
Seizure NA Not reported NA NA Jaramillo TC , et al. (2015)
Sensory Vision No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 3-5 months Jaramillo TC , et al. (2015)
Sensory Sensorimotor gating No change Prepulse inhibition 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social memory No change Reciprocal social interaction test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social interaction: with juveniles No change Reciprocal social interaction test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social interaction No change Reciprocal social interaction test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Social interaction No change Partition test 3-5 months Jaramillo TC , et al. (2015)
Social behavior Nest building behavior No change Nest building assay 3-5 months Jaramillo TC , et al. (2015)
Communications Ultrasonic vocalization: isolation induced Increased Monitoring ultrasonic vocalizations P4, p12 Jaramillo TC , et al. (2015)
Developmental profile NA Not reported NA NA Jaramillo TC , et al. (2015)
Emotion Thigmotaxis No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Open field test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Marble-burying test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Light-dark exploration test 3-5 months Jaramillo TC , et al. (2015)
Emotion Anxiety No change Elevated plus maze test 3-5 months Jaramillo TC , et al. (2015)
Immune response NA Not reported NA NA Jaramillo TC , et al. (2015)
Learning & memory Spatial reference memory No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Spatial reference memory Decreased Object-place recognition test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Spatial learning No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Object recognition memory Decreased Object-place recognition test 3-5 months Jaramillo TC , et al. (2015)
Learning & memory Cued or contextual fear conditioning No change Fear conditioning test NA Jaramillo TC , et al. (2015)
Learning & memory Cognitive flexibility No change Morris water maze test 3-5 months Jaramillo TC , et al. (2015)
Maternal behavior NA Not reported NA NA Jaramillo TC , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 3-6 months Jaramillo TC , et al. (2015)
Molecular profile Protein localization: synapse No change Western blot 3-6 months Jaramillo TC , et al. (2015)

M_SHANK3_15_KI_HM_INSG

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Autism-associated guanine nucleotide insertion mutation (Shank3InsG) was placed at position 3680 in exon 21, this insertion causes a frameshift and a stop codon immediately after the G insertion. The mice were maintained in C57B6/S129Sv background. Mice were bred to homozygosity normally.

Allele Type: ASD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv

Es cell line: R1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Zhou Y , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Zhou Y , et al. (2015)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test Adult Zhou Y , et al. (2015)
Motor phenotype General locomotor activity Decreased Open field test Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot P14 Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors Decreased Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot P14 Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Increased Western blot P14 Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Decreased Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Golgi-cox staining 2 months Zhou Y , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Presynaptic function No change Field potential recordings P14, adult Zhou Y , et al. (2015)
Neurophysiology Population spikes Decreased Field potential recordings P14 Zhou Y , et al. (2015)
Neurophysiology Population spikes Decreased Field potential recordings Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic currents: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory Increased Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments Adult Zhou Y , et al. (2015)
Seizure NA Not reported NA NA Zhou Y , et al. (2015)
Sensory Startle response: acoustic stimulus Decreased Acoustic startle reflex test Adult Zhou Y , et al. (2015)
Sensory Sensorimotor gating Decreased Prepulse inhibition Adult Zhou Y , et al. (2015)
Social behavior Social memory Decreased Three-chamber social approach test Adult Zhou Y , et al. (2015)
Social behavior Social dominance Increased Tube test of social dominance Adult Zhou Y , et al. (2015)
Social behavior Social approach Decreased Three-chamber social approach test Adult Zhou Y , et al. (2015)
Social behavior Juvenile play Decreased Reciprocal social interaction test 3 weeks Zhou Y , et al. (2015)
Communications Ultrasonic vocalization: isolation induced No change Monitoring ultrasonic vocalizations P2-p12 Zhou Y , et al. (2015)
Developmental profile NA Not reported NA NA Zhou Y , et al. (2015)
Emotion Exploratory activity: habituation No change Open field test Adult Zhou Y , et al. (2015)
Emotion Exploratory activity Decreased Open field test Adult Zhou Y , et al. (2015)
Emotion Anxiety Increased Open field test Adult Zhou Y , et al. (2015)
Emotion Anxiety Increased Elevated zero maze test Adult Zhou Y , et al. (2015)
Immune response NA Not reported NA NA Zhou Y , et al. (2015)
Learning & memory Spatial working memory No change T-maze test Adult Zhou Y , et al. (2015)
Maternal behavior NA Not reported NA NA Zhou Y , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 2 months Zhou Y , et al. (2015)
Molecular profile Targeted expression Decreased Quantitative pcr (qrt-pcr) 2 months Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse No change Western blot P14 Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot Adult Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot Adult Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot P14 Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Increased Western blot P14 Zhou Y , et al. (2015)
Molecular profile Protein expression level evidence Increased Western blot 2 months Zhou Y , et al. (2015)
Molecular profile Gene expression Increased Quantitative pcr (qrt-pcr) 2 months Zhou Y , et al. (2015)

M_SHANK3_15_KI_HT_INSG

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Autism-associated guanine nucleotide insertion mutation (Shank3InsG) was placed at position 3680 in exon 21, this insertion causes a frameshift and a stop codon immediately after the G insertion. The mice were maintained in C57B6/S129Sv background. Mice were bred to homozygosity.

Allele Type: ASD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv

Es cell line: R1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Zhou Y , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Zhou Y , et al. (2015)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test Adult Zhou Y , et al. (2015)
Motor phenotype General locomotor activity Decreased Open field test Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density No change Golgi-cox staining 2 months Zhou Y , et al. (2015)
Neurophysiology Presynaptic function No change Field potential recordings Adult Zhou Y , et al. (2015)
Neurophysiology Population spikes No change Field potential recordings Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments 5 months Zhou Y , et al. (2015)
Repetitive behavior Allogrooming: perseveration No change Grooming behavior assessments Adult Zhou Y , et al. (2015)
Seizure NA Not reported NA NA Zhou Y , et al. (2015)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test Adult Zhou Y , et al. (2015)
Sensory Sensorimotor gating No change Prepulse inhibition Adult Zhou Y , et al. (2015)
Social behavior Social dominance No change Tube test of social dominance Adult Zhou Y , et al. (2015)
Social behavior Social approach No change Three-chamber social approach test Adult Zhou Y , et al. (2015)
Communications NA Not reported NA NA Zhou Y , et al. (2015)
Developmental profile NA Not reported NA NA Zhou Y , et al. (2015)
Emotion Exploratory activity: habituation No change Open field test Adult Zhou Y , et al. (2015)
Emotion Exploratory activity Decreased Open field test Adult Zhou Y , et al. (2015)
Emotion Anxiety Increased Elevated zero maze test Adult Zhou Y , et al. (2015)
Immune response NA Not reported NA NA Zhou Y , et al. (2015)
Learning & memory NA Not reported NA NA Zhou Y , et al. (2015)
Maternal behavior NA Not reported NA NA Zhou Y , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 2 months Zhou Y , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 2 months Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot Adult Zhou Y , et al. (2015)

M_SHANK3_17_KI_HM_R1117X

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Schizophrenia associated non-sense mutation changing arginine to a stop codon (R1117X) was introduced by changing the codon for R 'CGG' to stop codon TGA in position 1117. Mice were maintained in C57Bl6*129Sv background and bred to homozygosity.

Allele Type: NDD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv

Es cell line: R1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Zhou Y , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Zhou Y , et al. (2015)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test Adult Zhou Y , et al. (2015)
Motor phenotype General locomotor activity Decreased Open field test Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot P14 Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors Decreased Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors Decreased Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot P14 Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot P14 Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Decreased Western blot Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Golgi-cox staining 2 months Zhou Y , et al. (2015)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Presynaptic function No change Field potential recordings P14, adult Zhou Y , et al. (2015)
Neurophysiology Population spikes Decreased Field potential recordings Adult Zhou Y , et al. (2015)
Neurophysiology Population spikes No change Field potential recordings P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic currents: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Increased Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp P14 Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments 5 months Zhou Y , et al. (2015)
Seizure NA Not reported NA NA Zhou Y , et al. (2015)
Sensory Startle response: acoustic stimulus Decreased Acoustic startle reflex test Adult Zhou Y , et al. (2015)
Sensory Sensorimotor gating Decreased Prepulse inhibition Adult Zhou Y , et al. (2015)
Social behavior Social memory Decreased Three-chamber social approach test Adult Zhou Y , et al. (2015)
Social behavior Social dominance Increased Tube test of social dominance Adult Zhou Y , et al. (2015)
Social behavior Social approach Decreased Three-chamber social approach test Adult Zhou Y , et al. (2015)
Social behavior Juvenile play No change Reciprocal social interaction test 3 weeks Zhou Y , et al. (2015)
Communications Ultrasonic vocalization: isolation induced No change Monitoring ultrasonic vocalizations P2-p12 Zhou Y , et al. (2015)
Developmental profile NA Not reported NA NA Zhou Y , et al. (2015)
Emotion Exploratory activity: habituation No change Open field test Adult Zhou Y , et al. (2015)
Emotion Exploratory activity Decreased Open field test Adult Zhou Y , et al. (2015)
Emotion Anxiety Increased Open field test Adult Zhou Y , et al. (2015)
Emotion Anxiety Increased Elevated zero maze test Adult Zhou Y , et al. (2015)
Immune response NA Not reported NA NA Zhou Y , et al. (2015)
Learning & memory Spatial working memory No change T-maze test Adult Zhou Y , et al. (2015)
Maternal behavior NA Not reported NA NA Zhou Y , et al. (2015)
Molecular profile Targeted expression Decreased Western blot 2 months Zhou Y , et al. (2015)
Molecular profile Targeted expression Decreased Quantitative pcr (qrt-pcr) 2 months Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot P14 Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse No change Western blot P14 Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot Adult Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot Adult Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot P14 Zhou Y , et al. (2015)
Molecular profile Protein expression level evidence No change Western blot 2 months Zhou Y , et al. (2015)
Molecular profile Gene expression No change Quantitative pcr (qrt-pcr) 2 months Zhou Y , et al. (2015)

M_SHANK3_17_KI_HT_R1117X

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Schizophrenia associated non-sense mutation changing arginine to a stop codon (R1117X) was introduced by changing the codon for R 'CGG' to stop codon TGA in position 1117. Mice were maintained in C57Bl6*129Sv background.

Allele Type: NDD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv

Es cell line: R1
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Zhou Y , et al. (2015)
Circadian sleep/wake cycle NA Not reported NA NA Zhou Y , et al. (2015)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test Adult Zhou Y , et al. (2015)
Motor phenotype General locomotor activity No change Open field test Adult Zhou Y , et al. (2015)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Golgi-cox staining 2 months Zhou Y , et al. (2015)
Neurophysiology Presynaptic function No change Field potential recordings Adult Zhou Y , et al. (2015)
Neurophysiology Population spikes No change Field potential recordings Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp Adult Zhou Y , et al. (2015)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments Adult Zhou Y , et al. (2015)
Repetitive behavior Allogrooming: perseveration Increased Grooming behavior assessments Adult Zhou Y , et al. (2015)
Seizure NA Not reported NA NA Zhou Y , et al. (2015)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test Adult Zhou Y , et al. (2015)
Sensory Sensorimotor gating No change Prepulse inhibition Adult Zhou Y , et al. (2015)
Social behavior Social dominance Increased Tube test of social dominance Adult Zhou Y , et al. (2015)
Social behavior Social approach Decreased Three-chamber social approach test Adult Zhou Y , et al. (2015)
Communications NA Not reported NA NA Zhou Y , et al. (2015)
Developmental profile NA Not reported NA NA Zhou Y , et al. (2015)
Emotion Exploratory activity: habituation No change Open field test Adult Zhou Y , et al. (2015)
Emotion Exploratory activity No change Open field test Adult Zhou Y , et al. (2015)
Emotion Anxiety Increased Elevated zero maze test Adult Zhou Y , et al. (2015)
Immune response NA Not reported NA NA Zhou Y , et al. (2015)
Learning & memory NA Not reported NA NA Zhou Y , et al. (2015)
Maternal behavior NA Not reported NA NA Zhou Y , et al. (2015)
Molecular profile Protein localization: synapse Decreased Western blot Adult Zhou Y , et al. (2015)

M_SHANK3_19_KO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Disruption of all murine Shank3 isoforms by cre/loxP mediated excision of exons 4-22 of the Shank3 gene, spanning 58 kb to include the coding sequences for all known Shank3 isoforms. The floxed mice were bred with CMV-cre mice to generate mice with deletions and bred till homozygosity. Authors report loss of all known Shank3 mRNA and protein.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Wang X , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Wang X , et al. (2016)
Motor phenotype Swimming ability No change Morris water maze test Adult Wang X , et al. (2016)
Motor phenotype Self grooming: artificial stress evoked Increased Grooming behavior assessments 4-7 months Wang X , et al. (2016)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test >2 months Wang X , et al. (2016)
Motor phenotype Grip strength No change Grip strength test NA Wang X , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity Decreased Home cage behavior Adult Wang X , et al. (2016)
Motor phenotype General locomotor activity Decreased Open field test Adult Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Thalamic morphology Increased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Post-synaptic density size Decreased Electron microscopy 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Post-synaptic density size No change Electron microscopy 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Olfactory bulb morphology Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: mglur Increased Western blot 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: mglur Increased Immunostaining 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Decreased Western blot 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the basal ganglia: striatum: caudoputamen Increased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the basal ganglia: lentiform nucleus: globus pallidus Increased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the amygdala Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology and size of the optic tract Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology and size of the corpus callosum Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology and size of stria terminalis Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology and size of fornix No change Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology and size of fimbria Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology and size of anterior commissure Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Hippocampal morphology Decreased Voxel based morphometry 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dti: fractional anisotropy or relative anisotropy in brain regions Decreased Diffusion tensor imaging (dti) 2-2.5 months Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Golgi-cox staining 8 weeks Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density No change Golgi-cox staining 8 weeks Wang X , et al. (2016)
Neurophysiology Synaptic plasticity: striatal ltd Decreased Whole-cell patch clamp 2-5 months Wang X , et al. (2016)
Neurophysiology Spontaneous post synaptic event frequency: excitatory currents Decreased Whole-cell patch clamp 2-5 months Wang X , et al. (2016)
Neurophysiology Spontaneous post synaptic event amplitude: excitatory currents No change Whole-cell patch clamp 2-5 months Wang X , et al. (2016)
Neurophysiology Presynaptic function Decreased High-frequency stimulation (hfs) 2-5 months Wang X , et al. (2016)
Neurophysiology Intrinsic membrane properties No change Whole-cell patch clamp 2-5 months Wang X , et al. (2016)
Neurophysiology Event related oscillations (eros) in electroencephalography (eeg): coherence Decreased In vivo local field potential (lfp) recordings 3-4 months Wang X , et al. (2016)
Neurophysiology Event related oscillations (eros) in electroencephalography (eeg) Decreased In vivo local field potential (lfp) recordings 3-4 months Wang X , et al. (2016)
Neurophysiology Action potential property: firing rate Increased Whole-cell patch clamp 2-5 months Wang X , et al. (2016)
Neurophysiology Action potential property: firing rate Decreased In vivo local field potential (lfp) recordings 3-4 months Wang X , et al. (2016)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments 4-7 months Wang X , et al. (2016)
Repetitive behavior Repetitive nose pokes No change Hole-board test 4-7 months Wang X , et al. (2016)
Seizure Seizures No change Observation of seizures Adult Wang X , et al. (2016)
Sensory Vision No change Morris water maze test Adult Wang X , et al. (2016)
Sensory Startle response: acoustic stimulus Decreased Acoustic startle reflex test Adult Wang X , et al. (2016)
Sensory Sensorimotor gating No change Prepulse inhibition Adult Wang X , et al. (2016)
Social behavior Social scent marking or recognition Decreased Homing test P15 Wang X , et al. (2016)
Social behavior Social interaction No change Reciprocal social interaction test >2 months Wang X , et al. (2016)
Social behavior Social dominance Decreased Dominance hierarchy formation >2 months Wang X , et al. (2016)
Social behavior Social approach No change Three-chamber social approach test >2 months Wang X , et al. (2016)
Social behavior Self grooming: social context Increased Grooming behavior assessments >2 months Wang X , et al. (2016)
Communications Ultrasonic vocalization: isolation induced Decreased Monitoring ultrasonic vocalizations P4 Wang X , et al. (2016)
Communications Ultrasonic vocalization: interaction induced: opposite sex stimulus Decreased Monitoring ultrasonic vocalizations Adult Wang X , et al. (2016)
Developmental profile Size/growth No change Body weight measurement P21, 3-6 months Wang X , et al. (2016)
Developmental profile Mortality/lethality No change General observations Adult Wang X , et al. (2016)
Developmental profile Developmental trajectory Decreased General observations P4 Wang X , et al. (2016)
Emotion Exploratory activity Decreased Novel cage test Adult Wang X , et al. (2016)
Emotion Exploratory activity Decreased Home cage behavior Adult Wang X , et al. (2016)
Emotion Anxiety Increased Light-dark exploration test Adult Wang X , et al. (2016)
Immune response NA Not reported NA NA Wang X , et al. (2016)
Learning & memory Spatial reference memory Decreased Morris water maze test Adult Wang X , et al. (2016)
Learning & memory Spatial learning No change Morris water maze test Adult Wang X , et al. (2016)
Learning & memory Reward reinforced choice behavior Decreased Operant conditioning paradigm Adult Wang X , et al. (2016)
Learning & memory Olfactory learning and memory No change Olfactory habituation-dishabituation test 2 months Wang X , et al. (2016)
Learning & memory Cued or contextual fear conditioning: memory of cue No change Fear conditioning test Adult Wang X , et al. (2016)
Learning & memory Cued or contextual fear conditioning: memory of context No change Fear conditioning test Adult Wang X , et al. (2016)
Learning & memory Cognitive flexibility Decreased Morris water maze test Adult Wang X , et al. (2016)
Maternal behavior NA Not reported NA NA Wang X , et al. (2016)
Molecular profile Protein phosphorylation Increased Western blot 8 weeks Wang X , et al. (2016)
Molecular profile Protein localization: synapse Decreased Western blot 8 weeks Wang X , et al. (2016)
Molecular profile Gene expression No change Quantitative pcr (qrt-pcr) 8 weeks Wang X , et al. (2016)

M_SHANK3_19_KO_HM_CDPPB-1

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

CDPPB ( SML0235, Sigma-Aldrich), dissolved in 0.5% methylcellulose, was administered at a dose of 10 mg/kg (i.p.) 30 min before behavioral testing in Shank3 exon 4-22 deleted homozygous mice (2 months age).

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Wang X , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Wang X , et al. (2016)
Motor phenotype NA Not reported NA NA Wang X , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Wang X , et al. (2016)
Neurophysiology Synaptic plasticity: striatal ltd Restored Whole-cell patch clamp 2-5 months Wang X , et al. (2016)
Neurophysiology Spontaneous post synaptic event frequency: excitatory currents Refractory Whole-cell patch clamp NA Wang X , et al. (2016)
Neurophysiology Spontaneous post synaptic event amplitude: excitatory currents No adverse effect Whole-cell patch clamp NA Wang X , et al. (2016)
Neurophysiology Presynaptic function Refractory High-frequency stimulation (hfs) NA Wang X , et al. (2016)
Neurophysiology Action potential property: firing rate Refractory Whole-cell patch clamp NA Wang X , et al. (2016)
Repetitive behavior Self grooming: perseveration Side effect Grooming behavior assessments 4-7 months Wang X , et al. (2016)
Seizure NA Not reported NA NA Wang X , et al. (2016)
Sensory NA Not reported NA NA Wang X , et al. (2016)
Social behavior NA Not reported NA NA Wang X , et al. (2016)
Communications NA Not reported NA NA Wang X , et al. (2016)
Developmental profile NA Not reported NA NA Wang X , et al. (2016)
Emotion NA Not reported NA NA Wang X , et al. (2016)
Immune response NA Not reported NA NA Wang X , et al. (2016)
Learning & memory NA Not reported NA NA Wang X , et al. (2016)
Maternal behavior NA Not reported NA NA Wang X , et al. (2016)
Molecular profile NA Not reported NA NA Wang X , et al. (2016)

M_SHANK3_19_KO_HM_CDPPB-2

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

CDPPB ( SML0235, Sigma-Aldrich), dissolved in 0.5% methylcellulose, was administered at a dose of 10 mg/kg (i.p.) for 7 days 30 min before behavioral testing,where mice were trained in a task every day, in Shank3 exon 4-22 deleted homozygous mice (2 months age).

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Wang X , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Wang X , et al. (2016)
Motor phenotype NA Not reported NA NA Wang X , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Wang X , et al. (2016)
Neurophysiology NA Not reported NA NA Wang X , et al. (2016)
Repetitive behavior NA Not reported NA NA Wang X , et al. (2016)
Seizure NA Not reported NA NA Wang X , et al. (2016)
Sensory NA Not reported NA NA Wang X , et al. (2016)
Social behavior NA Not reported NA NA Wang X , et al. (2016)
Communications NA Not reported NA NA Wang X , et al. (2016)
Developmental profile NA Not reported NA NA Wang X , et al. (2016)
Emotion NA Not reported NA NA Wang X , et al. (2016)
Immune response NA Not reported NA NA Wang X , et al. (2016)
Learning & memory Reward reinforced choice behavior Ameliorated Operant conditioning paradigm Adult Wang X , et al. (2016)
Maternal behavior NA Not reported NA NA Wang X , et al. (2016)
Molecular profile Protein localization: synapse Ameliorated Western blot Adult Wang X , et al. (2016)

M_SHANK3_19_KO_HM_MPEP

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

MPEP (M5343, Sigma-Aldrich), dissolved in 0.9% normal saline, was administered at a dose of 20mg/kg (i.p.) 30 min before behavioral testing in Shank3 exon 4-22 deleted homozygous mice (2 months of age). MPEP is a known mGlur5 antagonist.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Wang X , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Wang X , et al. (2016)
Motor phenotype General locomotor activity Restored Open field test Adult Wang X , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Wang X , et al. (2016)
Neurophysiology NA Not reported NA NA Wang X , et al. (2016)
Repetitive behavior Self grooming: perseveration Ameliorated Grooming behavior assessments 4-7 months Wang X , et al. (2016)
Seizure NA Not reported NA NA Wang X , et al. (2016)
Sensory NA Not reported NA NA Wang X , et al. (2016)
Social behavior NA Not reported NA NA Wang X , et al. (2016)
Communications NA Not reported NA NA Wang X , et al. (2016)
Developmental profile NA Not reported NA NA Wang X , et al. (2016)
Emotion NA Not reported NA NA Wang X , et al. (2016)
Immune response NA Not reported NA NA Wang X , et al. (2016)
Learning & memory NA Not reported NA NA Wang X , et al. (2016)
Maternal behavior NA Not reported NA NA Wang X , et al. (2016)
Molecular profile NA Not reported NA NA Wang X , et al. (2016)

M_SHANK3_19_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Shank3 exon 4-22 floxed mice were bred to CMV-cre mice to generate heterozygous Shank3 knockouts.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Wang X , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Wang X , et al. (2016)
Motor phenotype Grip strength No change Grip strength test Adult Wang X , et al. (2016)
Motor phenotype General locomotor activity No change Open field test Adult Wang X , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot 8 weeks Wang X , et al. (2016)
Neurophysiology NA Not reported NA NA Wang X , et al. (2016)
Repetitive behavior Repetitive nose pokes No change Hole-board test Adult Wang X , et al. (2016)
Seizure NA Not reported NA NA Wang X , et al. (2016)
Sensory Vision No change Morris water maze test Adult Wang X , et al. (2016)
Sensory Sensorimotor gating No change Prepulse inhibition Adult Wang X , et al. (2016)
Social behavior Social scent marking or recognition No change Homing test Adult Wang X , et al. (2016)
Social behavior Social memory No change Three-chamber social approach test Adult Wang X , et al. (2016)
Social behavior Social interaction No change Reciprocal social interaction test Adult Wang X , et al. (2016)
Social behavior Social approach No change Three-chamber social approach test Adult Wang X , et al. (2016)
Communications Ultrasonic vocalization: isolation induced No change Monitoring ultrasonic vocalizations P4 Wang X , et al. (2016)
Developmental profile NA Not reported NA NA Wang X , et al. (2016)
Emotion Anxiety No change Light-dark exploration test Adult Wang X , et al. (2016)
Immune response NA Not reported NA NA Wang X , et al. (2016)
Learning & memory Spatial reference memory No change Morris water maze test 8 weeks Wang X , et al. (2016)
Learning & memory Spatial learning No change Morris water maze test Adult Wang X , et al. (2016)
Learning & memory Olfactory learning and memory No change Olfactory habituation-dishabituation test Adult Wang X , et al. (2016)
Learning & memory Cognitive flexibility No change Morris water maze test Adult Wang X , et al. (2016)
Maternal behavior NA Not reported NA NA Wang X , et al. (2016)
Molecular profile Protein localization: synapse No change Western blot Adult Wang X , et al. (2016)

M_SHANK3_1_KO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene.

Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified

Es cell line: Bruce4 C57BL/6
Model Source: Not Specified
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Bozdagi O , et al. (2010)
Circadian sleep/wake cycle NA Not reported NA NA Bozdagi O , et al. (2010)
Motor phenotype NA Not reported NA NA Bozdagi O , et al. (2010)
Neuroanatomy / Ultrastructure / Cytoarchitecture Brain morphology No change Histology Unreported Bozdagi O , et al. (2010)
Neuroanatomy / Ultrastructure / Cytoarchitecture Brain morphology No change Gross necroscopy Unreported Bozdagi O , et al. (2010)
Neurophysiology NA Not reported NA NA Bozdagi O , et al. (2010)
Repetitive behavior NA Not reported NA NA Bozdagi O , et al. (2010)
Seizure Seizures No change General observations Unreported Bozdagi O , et al. (2010)
Sensory NA Not reported NA NA Bozdagi O , et al. (2010)
Social behavior NA Not reported NA NA Bozdagi O , et al. (2010)
Communications NA Not reported NA NA Bozdagi O , et al. (2010)
Developmental profile General characteristics No change General observations Unreported Bozdagi O , et al. (2010)
Developmental profile Developmental trajectory No change General observations P2-p14 Bozdagi O , et al. (2010)
Emotion NA Not reported NA NA Bozdagi O , et al. (2010)
Immune response NA Not reported NA NA Bozdagi O , et al. (2010)
Learning & memory NA Not reported NA NA Bozdagi O , et al. (2010)
Maternal behavior NA Not reported NA NA Bozdagi O , et al. (2010)
Molecular profile Protein expression level evidence Decreased Western blot Unreported Bozdagi O , et al. (2010)
Molecular profile Gene expression Decreased Quantitative pcr (qrt-pcr) Unreported Bozdagi O , et al. (2010)

M_SHANK3_1_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene.

Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified

Es cell line: Bruce4 C57BL/6
Model Source: Not Specified
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Bozdagi O , et al. (2013)
Physiological parameters NA Not reported NA NA Bozdagi O , et al. (2010)
Circadian sleep/wake cycle NA Not reported NA NA Bozdagi O , et al. (2013)
Circadian sleep/wake cycle NA Not reported NA NA Bozdagi O , et al. (2010)
Motor phenotype NA Not reported NA NA Bozdagi O , et al. (2013)
Motor phenotype NA Not reported NA NA Bozdagi O , et al. (2010)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Bozdagi O , et al. (2013)
Neuroanatomy / Ultrastructure / Cytoarchitecture Structural dendritic plasticity Abnormal Whole-cell patch clamp Unreported Bozdagi O , et al. (2010)
Neuroanatomy / Ultrastructure / Cytoarchitecture Brain morphology No change Histology Unreported Bozdagi O , et al. (2010)
Neuroanatomy / Ultrastructure / Cytoarchitecture Brain morphology No change Gross necroscopy Unreported Bozdagi O , et al. (2010)
Neurophysiology Synaptic plasticity: hippocampal ltp Decreased Whole-cell patch clamp Unreported Bozdagi O , et al. (2013)
Neurophysiology Neuroreceptor activity Decreased Whole-cell patch clamp Unreported Bozdagi O , et al. (2013)
Neurophysiology Synaptic transmission Decreased Field potential recordings 3-4 weeks Bozdagi O , et al. (2010)
Neurophysiology Synaptic plasticity: hippocampal ltp Decreased Field potential recordings Unreported Bozdagi O , et al. (2010)
Neurophysiology Synaptic plasticity: hippocampal ltd No change Field potential recordings Unreported Bozdagi O , et al. (2010)
Neurophysiology Presynaptic function: paired-pulse facilitation Decreased Whole-cell patch clamp 3 months Bozdagi O , et al. (2010)
Neurophysiology Miniature post synaptic current frequency: excitatory Increased Whole-cell patch clamp NA Bozdagi O , et al. (2010)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp 3 months Bozdagi O , et al. (2010)
Repetitive behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Repetitive behavior NA Not reported NA NA Bozdagi O , et al. (2010)
Seizure NA Not reported NA NA Bozdagi O , et al. (2013)
Seizure Seizures No change General observations Unreported Bozdagi O , et al. (2010)
Sensory NA Not reported NA NA Bozdagi O , et al. (2013)
Sensory Olfaction No change Olfactory habituation-dishabituation test 2.5-4 months Bozdagi O , et al. (2010)
Social behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Social behavior Social interaction: opposite sex Decreased Reciprocal social interaction test 2.5-4 months Bozdagi O , et al. (2010)
Communications NA Not reported NA NA Bozdagi O , et al. (2013)
Communications Ultrasonic vocalization: interaction induced: opposite sex stimulus Decreased Monitoring ultrasonic vocalizations 2.5-4 months Bozdagi O , et al. (2010)
Developmental profile NA Not reported NA NA Bozdagi O , et al. (2013)
Developmental profile General characteristics No change General observations Unreported Bozdagi O , et al. (2010)
Developmental profile Developmental trajectory No change General observations P2-p14 Bozdagi O , et al. (2010)
Emotion NA Not reported NA NA Bozdagi O , et al. (2013)
Emotion NA Not reported NA NA Bozdagi O , et al. (2010)
Immune response NA Not reported NA NA Bozdagi O , et al. (2013)
Immune response NA Not reported NA NA Bozdagi O , et al. (2010)
Learning & memory NA Not reported NA NA Bozdagi O , et al. (2013)
Learning & memory NA Not reported NA NA Bozdagi O , et al. (2010)
Maternal behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Maternal behavior NA Not reported NA NA Bozdagi O , et al. (2010)
Molecular profile NA Not reported NA NA Bozdagi O , et al. (2013)
Molecular profile Protein expression level evidence Decreased Western blot Unreported Bozdagi O , et al. (2010)
Molecular profile Protein expression level evidence Decreased Western blot 3 months Bozdagi O , et al. (2010)
Molecular profile Gene expression Decreased Quantitative pcr (qrt-pcr) Unreported Bozdagi O , et al. (2010)

M_SHANK3_1_KO_HT_IGF1-H

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene followed by intraperitoneal injection at 240 microg/kg/day starting at PND 13-15 for 2 weeks of full-length IGF-1.

Allele Type: Partial knockout
Strain of Origin: Not specified
Genetic Background: Not Specified

Es cell line: Not specified
Model Source: Not Specified
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Bozdagi O , et al. (2013)
Circadian sleep/wake cycle NA Not reported NA NA Bozdagi O , et al. (2013)
Motor phenotype Motor coordination and balance Restored Accelerating rotarod test 4 weeks Bozdagi O , et al. (2013)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Bozdagi O , et al. (2013)
Neurophysiology Synaptic plasticity: hippocampal ltp Restored Whole-cell patch clamp 4 weeks Bozdagi O , et al. (2013)
Neurophysiology Neuroreceptor activity Restored Whole-cell patch clamp 4 weeks Bozdagi O , et al. (2013)
Repetitive behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Seizure NA Not reported NA NA Bozdagi O , et al. (2013)
Sensory NA Not reported NA NA Bozdagi O , et al. (2013)
Social behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Communications NA Not reported NA NA Bozdagi O , et al. (2013)
Developmental profile NA Not reported NA NA Bozdagi O , et al. (2013)
Emotion NA Not reported NA NA Bozdagi O , et al. (2013)
Immune response NA Not reported NA NA Bozdagi O , et al. (2013)
Learning & memory NA Not reported NA NA Bozdagi O , et al. (2013)
Maternal behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Molecular profile NA Not reported NA NA Bozdagi O , et al. (2013)

M_SHANK3_1_KO_HT_IGF1-L

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene followed by intraperitoneal injection at 120 microg/kg/day starting at PND 13-15 for 2 weeks of full-length IGF-1.

Allele Type: Partial knockout
Strain of Origin: Not specified
Genetic Background: Not Specified

Es cell line: Not specified
Model Source: Not Specified
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Bozdagi O , et al. (2013)
Circadian sleep/wake cycle NA Not reported NA NA Bozdagi O , et al. (2013)
Motor phenotype NA Not reported NA NA Bozdagi O , et al. (2013)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Bozdagi O , et al. (2013)
Neurophysiology Synaptic plasticity: hippocampal ltp Ameliorated Whole-cell patch clamp 4 weeks Bozdagi O , et al. (2013)
Repetitive behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Seizure NA Not reported NA NA Bozdagi O , et al. (2013)
Seizure Absence seizures Ameliorated 16s rrna gene sequence analysis for identification of bacteria Dfsd Bozdagi O , et al. (2013)
Sensory NA Not reported NA NA Bozdagi O , et al. (2013)
Social behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Communications NA Not reported NA NA Bozdagi O , et al. (2013)
Developmental profile NA Not reported NA NA Bozdagi O , et al. (2013)
Emotion NA Not reported NA NA Bozdagi O , et al. (2013)
Immune response NA Not reported NA NA Bozdagi O , et al. (2013)
Learning & memory NA Not reported NA NA Bozdagi O , et al. (2013)
Maternal behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Molecular profile NA Not reported NA NA Bozdagi O , et al. (2013)

M_SHANK3_1_KO_HT_PIGF1

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene followed by intraperitoneal injections at 10 microg/g/day for 2 weeks of active peptide derivative of IGF-1.

Allele Type: Partial knockout
Strain of Origin: Not specified
Genetic Background: Not Specified

Es cell line: Not specified
Model Source: Not Specified
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Bozdagi O , et al. (2013)
Circadian sleep/wake cycle NA Not reported NA NA Bozdagi O , et al. (2013)
Motor phenotype NA Not reported NA NA Bozdagi O , et al. (2013)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Bozdagi O , et al. (2013)
Neurophysiology Synaptic plasticity: hippocampal ltp Restored Whole-cell patch clamp 4 weeks Bozdagi O , et al. (2013)
Neurophysiology Neuroreceptor activity Restored Whole-cell patch clamp 4 weeks Bozdagi O , et al. (2013)
Repetitive behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Seizure NA Not reported NA NA Bozdagi O , et al. (2013)
Sensory NA Not reported NA NA Bozdagi O , et al. (2013)
Social behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Communications NA Not reported NA NA Bozdagi O , et al. (2013)
Developmental profile NA Not reported NA NA Bozdagi O , et al. (2013)
Emotion NA Not reported NA NA Bozdagi O , et al. (2013)
Immune response NA Not reported NA NA Bozdagi O , et al. (2013)
Learning & memory NA Not reported NA NA Bozdagi O , et al. (2013)
Maternal behavior NA Not reported NA NA Bozdagi O , et al. (2013)
Molecular profile NA Not reported NA NA Bozdagi O , et al. (2013)

M_SHANK3_20_CKO_HM_NAC

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Conditional deletion of exons 4-22 of the Shank3 gene in 10-12 week old female mice by injecting AAV10- Cre virus, bilaterally, in the nucleus accumbens (NAc) to selectively knockdown Shank3 in the Nac

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Wang X , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Wang X , et al. (2016)
Motor phenotype NA Not reported NA NA Wang X , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Wang X , et al. (2016)
Neurophysiology Action potential property: firing rate Decreased In vivo local field potential (lfp) recordings 3-4 months Wang X , et al. (2016)
Repetitive behavior NA Not reported NA NA Wang X , et al. (2016)
Seizure NA Not reported NA NA Wang X , et al. (2016)
Sensory NA Not reported NA NA Wang X , et al. (2016)
Social behavior NA Not reported NA NA Wang X , et al. (2016)
Communications NA Not reported NA NA Wang X , et al. (2016)
Developmental profile NA Not reported NA NA Wang X , et al. (2016)
Emotion NA Not reported NA NA Wang X , et al. (2016)
Immune response NA Not reported NA NA Wang X , et al. (2016)
Learning & memory NA Not reported NA NA Wang X , et al. (2016)
Maternal behavior NA Not reported NA NA Wang X , et al. (2016)
Molecular profile NA Not reported NA NA Wang X , et al. (2016)

M_SHANK3_21_KI_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Targeted knockin inversion of exons 13 to 16, that include the coding region of the PDZ domain, generated using the FLEx genetic switch strategy. The inverted insert is flanked by FRT and loxP sites. The knockin floxed mouse is called a functional knockout by authors.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Physiological parameters NA Not reported NA NA Mei Y , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Mei Y , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity Decreased Open field test 2-3 months Guo B , et al. (2019)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test Unreported Mei Y , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity Decreased Open field test Unreported Mei Y , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors Decreased Western blot 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Decreased Western blot 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine morphology Decreased Immunofluorescence staining 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Immunofluorescence staining 2-3 months Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Mei Y , et al. (2016)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Increased Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Epsp-spike relationship Decreased Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Presynaptic function: presynaptic fiber volley No change Field potential recordings 3-7 months Mei Y , et al. (2016)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Neurophysiology Population spikes Decreased Field potential recordings 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments 2-3 months Guo B , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 2-3 months Guo B , et al. (2019)
Repetitive behavior Self grooming: perseveration Increased General observations Unreported Mei Y , et al. (2016)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Seizure NA Not reported NA NA Mei Y , et al. (2016)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Mei Y , et al. (2016)
Social behavior Social interaction Decreased Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Social behavior NA Not reported NA NA Mei Y , et al. (2016)
Communications NA Not reported NA NA Guo B , et al. (2019)
Communications NA Not reported NA NA Mei Y , et al. (2016)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Mei Y , et al. (2016)
Emotion Exploratory activity Decreased Novel object recognition test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Increased Open field test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Increased Zero maze test Unreported Mei Y , et al. (2016)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Immune response NA Not reported NA NA Mei Y , et al. (2016)
Learning & memory Object recognition memory Decreased Novel object recognition test 2-3 months Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Mei Y , et al. (2016)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Mei Y , et al. (2016)
Molecular profile Targeted expression Decreased Western blot 2-3 months Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)
Molecular profile Targeted expression Decreased Western blot Unreported Mei Y , et al. (2016)

M_SHANK3_21_KI_HM_REINSTATE-ADULT

Model Type: RESCUE-Genetic
Model Genotype: Homozygous

Mutation:

Conditional knock-in Shank3 mouse model in which exon 13 - 16 encoding the PDZ domain, were inverted with a Flex system were injected with AAV2/8-CaMKII-Cre-EGFP in the ACC to re-express most SHANK3 isoforms in the ACC but not the adjacent motor cortex.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity Refractory Open field test 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors Restored Western blot 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Ameliorated Western blot 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine morphology Restored Immunofluorescence staining 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Restored Immunofluorescence staining 2-3 months Guo B , et al. (2019)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Restored Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Presynaptic function: paired-pulse facilitation No adverse effect Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current frequency: excitatory Ameliorated Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current amplitude: excitatory Restored Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Neurophysiology Epsp-spike relationship Restored Whole-cell patch clamp 2-3 months Guo B , et al. (2019)
Repetitive behavior Self grooming: perseveration Refractory Grooming behavior assessments 2-3 months Guo B , et al. (2019)
Repetitive behavior Repetitive digging Refractory Marble-burying test 2-3 months Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Ameliorated Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion Exploratory activity Refractory Novel object recognition test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Refractory Elevated plus maze test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Refractory Open field test 2-3 months Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory Object recognition memory Refractory Novel object recognition test 2-3 months Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile Targeted expression Restored Western blot 2-3 months Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_22_KI_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Mice with inverted exons 13-16 and the transgene CAGGS-CreER used as controls or parent models. Genotype of these mice is: Shank3fx/fx;CreER+/-.Mice received corn oil treatment (vehicle) at 2-4.5 months.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Mei Y , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Mei Y , et al. (2016)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test Adult, p20-21 Mei Y , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity Decreased Open field test Adult, p20-21 Mei Y , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Immunohistochemistry Adult, p20-21 Mei Y , et al. (2016)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Neurophysiology Presynaptic function: presynaptic fiber volley No change Field potential recordings 3-7 months Mei Y , et al. (2016)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Neurophysiology Population spikes Decreased Field potential recordings 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments Adult, p20-21 Mei Y , et al. (2016)
Seizure NA Not reported NA NA Mei Y , et al. (2016)
Sensory NA Not reported NA NA Mei Y , et al. (2016)
Social behavior Social approach Decreased Three-chamber social approach test Adult, p20-21 Mei Y , et al. (2016)
Social behavior Rearing behavior Decreased Open field test NA Mei Y , et al. (2016)
Communications NA Not reported NA NA Mei Y , et al. (2016)
Developmental profile Size/growth Decreased Body weight measurement P20-21 Mei Y , et al. (2016)
Emotion Anxiety Increased Elevated zero maze test Adult, p20-21 Mei Y , et al. (2016)
Immune response NA Not reported NA NA Mei Y , et al. (2016)
Learning & memory NA Not reported NA NA Mei Y , et al. (2016)
Maternal behavior NA Not reported NA NA Mei Y , et al. (2016)
Molecular profile Targeted expression Decreased Western blot Adult, p20-21 Mei Y , et al. (2016)
Molecular profile Protein expression level evidence No change Western blot Adult, p20-21 Mei Y , et al. (2016)
Molecular profile Protein expression level evidence Increased Western blot Adult, p20-21 Mei Y , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot Adult, p20-21 Mei Y , et al. (2016)

M_SHANK3_22_KI_HM_REINSTATE-ADULT

Model Type: RESCUE-Genetic
Model Genotype: Homozygous

Mutation:

Mice with the inverted exons 13-16 re-inverted using tamoxifen-inducible globally expressed CAGGS-CreER. The pCAGGS promoter includes the CMV early enhancer with chicken beta-actin promoter. Adult rescue was performed by tamoxifen treament by oral gavaging at 2-4.5 months while control group received corn oil. Genotype of mice: Shank3fx/fx;CreER+/-.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Mei Y , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Mei Y , et al. (2016)
Motor phenotype Motor coordination and balance Refractory Accelerating rotarod test Adult Mei Y , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity Refractory Open field test Adult Mei Y , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No adverse effect Whole-cell patch clamp 3-7 months, p20-21 Mei Y , et al. (2016)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Restored Immunohistochemistry Adult Mei Y , et al. (2016)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No adverse effect Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Presynaptic function: presynaptic fiber volley No adverse effect Field potential recordings 3-7 months Mei Y , et al. (2016)
Neurophysiology Presynaptic function: paired-pulse facilitation No adverse effect Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Population spikes Restored Field potential recordings 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current frequency: excitatory Restored Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Neurophysiology Miniature post synaptic current amplitude: excitatory No adverse effect Whole-cell patch clamp 3-7 months Mei Y , et al. (2016)
Repetitive behavior Self grooming: perseveration Restored Grooming behavior assessments Adult Mei Y , et al. (2016)
Seizure NA Not reported NA NA Mei Y , et al. (2016)
Sensory NA Not reported NA NA Mei Y , et al. (2016)
Social behavior Social approach Ameliorated Three-chamber social approach test Adult Mei Y , et al. (2016)
Social behavior Rearing behavior Refractory Open field test Adult Mei Y , et al. (2016)
Communications NA Not reported NA NA Mei Y , et al. (2016)
Developmental profile NA Not reported NA NA Mei Y , et al. (2016)
Emotion Anxiety Refractory Elevated zero maze test Adult Mei Y , et al. (2016)
Immune response NA Not reported NA NA Mei Y , et al. (2016)
Learning & memory NA Not reported NA NA Mei Y , et al. (2016)
Maternal behavior NA Not reported NA NA Mei Y , et al. (2016)
Molecular profile Targeted expression Ameliorated Immunohistochemistry Adult Mei Y , et al. (2016)
Molecular profile Protein expression level evidence Restored Western blot Adult Mei Y , et al. (2016)

M_SHANK3_22_KI_HM_REINSTATE-JUVENILE

Model Type: RESCUE-Genetic
Model Genotype: Homozygous

Mutation:

Mice with the inverted exons 13-16 re-inverted using tamoxifen-inducible globally expressed CAGGS-CreER. The pCAGGS promoter includes the CMV early enhancer with chicken beta-actin promoter. Juvenile rescue was performed by tamoxifen treament by oral gavaging at P20 to 21, while control group received corn oil. Genotype of mice: Shank3fx/fx;CreER+/-.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Mei Y , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Mei Y , et al. (2016)
Motor phenotype Motor coordination and balance Restored Accelerating rotarod test Adult Mei Y , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity Refractory Open field test Adult Mei Y , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Mei Y , et al. (2016)
Neurophysiology NA Not reported NA NA Mei Y , et al. (2016)
Repetitive behavior NA Not reported NA NA Mei Y , et al. (2016)
Seizure NA Not reported NA NA Mei Y , et al. (2016)
Sensory NA Not reported NA NA Mei Y , et al. (2016)
Social behavior Rearing behavior Refractory Open field test Adult Mei Y , et al. (2016)
Communications NA Not reported NA NA Mei Y , et al. (2016)
Developmental profile Size/growth Side effect Body weight measurement P20-21 Mei Y , et al. (2016)
Emotion Anxiety Restored Elevated zero maze test Adult Mei Y , et al. (2016)
Immune response NA Not reported NA NA Mei Y , et al. (2016)
Learning & memory NA Not reported NA NA Mei Y , et al. (2016)
Maternal behavior NA Not reported NA NA Mei Y , et al. (2016)
Molecular profile Targeted expression Ameliorated Western blot Adult Mei Y , et al. (2016)

M_SHANK3_23_KI_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

KI of neo-STOP cassette inserted in intron 12, between exon 12 and exon 13, resulting in loss of higher mol weight Shank3 isoforms, bred to homozygosity. All Shank3 protein expressed in these mutants lacks the PDX domain.

Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57BL/6J

Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Jaramillo TC , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Jaramillo TC , et al. (2016)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Motor phenotype Motor coordination and balance Decreased Accelerating rotarod test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Motor phenotype General locomotor activity No change Open field test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Jaramillo TC , et al. (2016)
Neurophysiology Synaptic transmission No change Field potential recordings 4-6 weeks Jaramillo TC , et al. (2016)
Neurophysiology Synaptic plasticity: hippocampal ltp Decreased Whole-cell patch clamp 4-6 weeks Jaramillo TC , et al. (2016)
Neurophysiology Synaptic plasticity: hippocampal ltd No change Whole-cell patch clamp 4-6 weeks Jaramillo TC , et al. (2016)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Decreased Whole-cell patch clamp 4-6 weeks Jaramillo TC , et al. (2016)
Neurophysiology Presynaptic function: paired-pulse depression (ppd) No change Paired-pulse ratio 4-6 weeks Jaramillo TC , et al. (2016)
Neurophysiology Epsp-spike relationship No change Field potential recordings 4-6 weeks Jaramillo TC , et al. (2016)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments 5.4- 9 weeks Jaramillo TC , et al. (2016)
Repetitive behavior Repetitive digging Decreased Marble-burying test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Seizure NA Not reported NA NA Jaramillo TC , et al. (2016)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Sensory Sensorimotor gating No change Prepulse inhibition 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social memory: long-term social memory Decreased Social recognition test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social memory Decreased Three-chamber social approach test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social interaction Decreased Partition test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social interaction Decreased Reciprocal social interaction test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social approach No change Three-chamber social approach test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Communications NA Not reported NA NA Jaramillo TC , et al. (2016)
Developmental profile NA Not reported NA NA Jaramillo TC , et al. (2016)
Emotion Thigmotaxis Increased Morris water maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Exploratory activity Decreased Home cage behavior 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Anxiety No change Open field test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Anxiety No change Elevated plus maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Anxiety No change Light-dark exploration test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Immune response NA Not reported NA NA Jaramillo TC , et al. (2016)
Learning & memory Spatial reference memory Decreased Morris water maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Learning & memory Spatial learning Decreased Morris water maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Maternal behavior NA Not reported NA NA Jaramillo TC , et al. (2016)
Molecular profile Targeted expression Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)

M_SHANK3_23_KI_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Neo-STOP cassette was inserted in intron 12, between exon 12 and exon 13, of the Shank3 gene and results in the loss of two higher molecular weight isoforms of Shank3, and results in a protein lacking the PDZ domain.

Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57BL/6J

Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Jaramillo TC , et al. (2016)
Circadian sleep/wake cycle NA Not reported NA NA Jaramillo TC , et al. (2016)
Motor phenotype Motor coordination and balance Increased Accelerating rotarod test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Motor phenotype General locomotor activity No change Open field test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Jaramillo TC , et al. (2016)
Neurophysiology Synaptic plasticity: hippocampal ltp Decreased Whole-cell patch clamp 4-6 weeks Jaramillo TC , et al. (2016)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Decreased Whole-cell patch clamp 4-6 weeks Jaramillo TC , et al. (2016)
Repetitive behavior Self grooming: perseveration Increased Grooming behavior assessments 5.4- 9 weeks Jaramillo TC , et al. (2016)
Repetitive behavior Repetitive digging No change Marble-burying test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Seizure NA Not reported NA NA Jaramillo TC , et al. (2016)
Sensory NA Not reported NA NA Jaramillo TC , et al. (2016)
Social behavior Social memory: long-term social memory Decreased Social recognition test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social memory Decreased Three-chamber social approach test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social interaction No change Partition test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social interaction No change Reciprocal social interaction test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Social behavior Social approach Decreased Three-chamber social approach test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Communications NA Not reported NA NA Jaramillo TC , et al. (2016)
Developmental profile NA Not reported NA NA Jaramillo TC , et al. (2016)
Emotion Thigmotaxis No change Morris water maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Exploratory activity No change Home cage behavior 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Exploratory activity Decreased Home cage behavior 5.4- 9 weeks Jaramillo TC , et al. (2016)
Emotion Anxiety No change Light-dark exploration test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Immune response NA Not reported NA NA Jaramillo TC , et al. (2016)
Learning & memory Spatial reference memory No change Morris water maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Learning & memory Spatial learning No change Morris water maze test 5.4- 9 weeks Jaramillo TC , et al. (2016)
Maternal behavior NA Not reported NA NA Jaramillo TC , et al. (2016)
Molecular profile Targeted expression Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)
Molecular profile Protein expression level evidence Decreased Western blot 4 weeks Jaramillo TC , et al. (2016)

M_SHANK3_24_KD

Model Type: Genetic
Model Genotype: Wildtype

Mutation:

Seven-week-old wildtype C57BL/6 male mice were injected with Shank3-siRNA (1424750, NM_021423.2), bilaterally in the dorsal striatum, using a stereotaxic apparatus, resulting in a reduction of Shank3 transcript levels to 0.4 fold compared with control siRNA injected mice.

Allele Type: Knockdown
Strain of Origin: C57BL/6
Genetic Background: C57BL/6

Es cell line: NA
Model Source: Daehan BioLink (Eumsung, Chungbuk, Republic of Korea)
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Lee Y , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Lee Y , et al. (2019)
Motor phenotype NA Not reported NA NA Lee Y , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Lee Y , et al. (2019)
Neurophysiology NA Not reported NA NA Lee Y , et al. (2019)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments Adult Lee Y , et al. (2019)
Repetitive behavior Repetitive digging No change Home cage behavior Adult Lee Y , et al. (2019)
Seizure NA Not reported NA NA Lee Y , et al. (2019)
Sensory NA Not reported NA NA Lee Y , et al. (2019)
Social behavior Social memory No change U-field assay Adult Lee Y , et al. (2019)
Communications NA Not reported NA NA Lee Y , et al. (2019)
Developmental profile NA Not reported NA NA Lee Y , et al. (2019)
Emotion NA Not reported NA NA Lee Y , et al. (2019)
Immune response NA Not reported NA NA Lee Y , et al. (2019)
Learning & memory Object recognition memory Decreased Object preference test Adult Lee Y , et al. (2019)
Maternal behavior NA Not reported NA NA Lee Y , et al. (2019)
Molecular profile Targeted expression Decreased Quantitative pcr (qrt-pcr) Adult Lee Y , et al. (2019)

M_SHANK3_24_KI_HM_S685I

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Mice with autism-linked human missense mutation S685I knocked in using CRISPR-CAS9. A FLAG-tagged Shank3 construct is used.

Allele Type: ASD LOF mutation
Strain of Origin: FVB/N
Genetic Background: FVB

Es cell line: FVB/N
Model Source: PMID 30610205
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters Reproductive function No change General observations Adult Wang L , et al. (2019)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test Adult Wang L , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test Adult Wang L , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Post-synaptic density size No change Western blot Adult Wang L , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density No change Immunostaining Adult Wang L , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Immunostaining Adult Wang L , et al. (2019)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp Adult Wang L , et al. (2019)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp Adult Wang L , et al. (2019)
Repetitive behavior Vertical jumping or back flipping No change Open field test Adult Wang L , et al. (2019)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments Adult Wang L , et al. (2019)
Repetitive behavior Repetitive digging No change Marble-burying test Adult Wang L , et al. (2019)
Repetitive behavior Allogrooming: perseveration Increased Resident-intruder test Adult Wang L , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test Adult Wang L , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition Adult Wang L , et al. (2019)
Social behavior Social interaction: opposite sex No change Resident-intruder test Adult Wang L , et al. (2019)
Social behavior Social dominance Increased Tube test of social dominance Adult Wang L , et al. (2019)
Social behavior Social approach No change Three-chamber social approach test Adult Wang L , et al. (2019)
Social behavior Aggression No change Resident-intruder test Adult Wang L , et al. (2019)
Communications Ultrasonic vocalization: isolation induced Abnormal Monitoring ultrasonic vocalizations P2-p16 Wang L , et al. (2019)
Communications Ultrasonic vocalization: interaction induced: opposite sex stimulus No change Monitoring ultrasonic vocalizations Adult Wang L , et al. (2019)
Developmental profile Size/growth No change Body weight measurement 0-12 weeks Wang L , et al. (2019)
Developmental profile Mortality/lethality No change General observations P0 Wang L , et al. (2019)
Developmental profile Developmental trajectory No change General observations P13-p14 Wang L , et al. (2019)
Emotion Anxiety No change Open field test Adult Wang L , et al. (2019)
Emotion Anxiety No change Elevated plus maze test Adult Wang L , et al. (2019)
Molecular profile Targeted expression No change Western blot Adult Wang L , et al. (2019)
Molecular profile Protein expression level evidence No change Western blot Adult Wang L , et al. (2019)
Molecular profile Protein expression level evidence Decreased Western blot Adult Wang L , et al. (2019)
Molecular profile Protein binding No change Immunoprecipitation Adult Wang L , et al. (2019)
Molecular profile Protein binding Decreased Immunoprecipitation Adult Wang L , et al. (2019)

M_SHANK3_25_KI_HT_S685I

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Heterozygous mice with autism-linked human missennse mutation S685I knocked in using CRISPR-CAS9. FLAG-tagged Shank3 construct is used.

Allele Type: ASD LOF mutation
Strain of Origin: FVB/N
Genetic Background: FVB

Es cell line: FVB/N
Model Source: PMID 30610205
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters Reproductive function No change General observations Adult Wang L , et al. (2019)
Motor phenotype Motor coordination and balance No change Accelerating rotarod test Adult Wang L , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test Adult Wang L , et al. (2019)
Repetitive behavior Vertical jumping or back flipping No change Open field test Adult Wang L , et al. (2019)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments Adult Wang L , et al. (2019)
Repetitive behavior Repetitive digging No change Marble-burying test Adult Wang L , et al. (2019)
Repetitive behavior Allogrooming: perseveration No change Resident-intruder test Adult Wang L , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test Adult Wang L , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition Adult Wang L , et al. (2019)
Social behavior Social dominance No change Tube test of social dominance Adult Wang L , et al. (2019)
Social behavior Social dominance Increased Tube test of social dominance Adult Wang L , et al. (2019)
Social behavior Social approach No change Three-chamber social approach test Adult Wang L , et al. (2019)
Communications Ultrasonic vocalization: isolation induced No change Monitoring ultrasonic vocalizations P2-p16 Wang L , et al. (2019)
Communications Ultrasonic vocalization: interaction induced: opposite sex stimulus No change Monitoring ultrasonic vocalizations Adult Wang L , et al. (2019)
Developmental profile Size/growth No change Body weight measurement 0-12 weeks Wang L , et al. (2019)
Developmental profile Mortality/lethality No change General observations P0 Wang L , et al. (2019)
Emotion Anxiety No change Open field test Adult Wang L , et al. (2019)
Emotion Anxiety No change Elevated plus maze test Adult Wang L , et al. (2019)
Molecular profile Protein binding Decreased Immunoprecipitation Adult Wang L , et al. (2019)

M_SHANK3_25_KO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Model with exons 13 to 16 (containing the PDZ domain) of the Shank3 gene replaced with a NEO cassette. This model is identical to the construct of M_SHANK3_4_KO_HM but on a different genetic background; M_SHANK3_4_KO_HM

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA ()
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA ()
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA ()
Motor phenotype General locomotor activity: ambulatory activity Decreased Open field test 2-3 months Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA ()
Neuroanatomy / Ultrastructure / Cytoarchitecture Post-synaptic density size Decreased Immunofluorescence staining 2-6 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot 2-6 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors No change Western blot 2-6 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Decreased Western blot 2-6 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine morphology Decreased Fluorescence microscopy 2-6 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: spine density Decreased Fluorescence microscopy 2-6 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Dendritic architecture: dendritic tree complexity Decreased Sholl analysis 2-6 months Guo B , et al. (2019)
Neurophysiology Neuronal activation Increased Immunohistochemistry Adult, 2-5 months, 9-12 months ()
Neurophysiology Synaptic transmission: excitatory No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Synaptic plasticity: anterior cingulate cortex ltp Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Increased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Neuronal activation: non-familiar social interaction Decreased Fiber photometry 2-6 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current frequency: excitatory No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current amplitude: excitatory No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Membrane potential No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Intrinsic membrane properties No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Epsp-spike relationship Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Action potential property: threshold Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Action potential property: firing rate No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Action potential property: amplitude No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Action potential properties: half-width No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Repetitive behavior NA Not reported NA NA ()
Repetitive behavior Repetitive digging Decreased Marble-burying test 2-3 months Guo B , et al. (2019)
Seizure NA Not reported NA NA ()
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA ()
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test Adult ()
Social behavior Social interaction: with juveniles Decreased Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA ()
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA ()
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion NA Not reported NA NA ()
Emotion Exploratory activity Decreased Novel object recognition test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Increased Open field test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 2-3 months Guo B , et al. (2019)
Immune response NA Not reported NA NA ()
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA ()
Learning & memory Object recognition memory Decreased Novel object recognition test 2-3 months Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA ()
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA ()
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_ACC-ACT-1

Model Type: RESCUE-Procedural
Model Genotype: Homozygous

Mutation:

Shank3 null mice with knockout of the PDZ domain were injected AAV2/8-CaMKIIa-hChR2-EYFP to deliver channelrhodopsin-2 (ChR2) to pyramidal neurons in the ACC to selectively enhance the activity of ACC pyramidal neurons transiently. Whole-cell recordings show that ACC pyramidal neurons exhibit activation in response to 10 Hz of optogenetic stimulation with a 473-nm laser. Control Shank3 null mice were injected EYFP.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Guo B , et al. (2019)
Neurophysiology Neuronal activation Restored Immunohistochemistry 2-3 months Guo B , et al. (2019)
Repetitive behavior NA Not reported NA NA Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Restored Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion Anxiety Restored Open field test 2-3 months Guo B , et al. (2019)
Emotion Anxiety Restored Elevated plus maze test 2-3 months Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_ACC-ACT-2

Model Type: RESCUE-Procedural
Model Genotype: Homozygous

Mutation:

Shank3 KO mice were injected with a mixture of AAV2/8-CaMKIIa-Cre-EGFP and AAV2/8-EF1a-DIO-hM3D(Gq)-mCherry into the ACC and expression of hM3Dq designer receptors was verified in ACC pyramidal neurons that are exclusively activated by designer drugs (DREADDs) to enhance ACC pyramidal cell activity over extended periods. 0.7 mg per kg clozapine N-oxide (CNO) was administered before the test to activate ACC pyramidal neurons.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Guo B , et al. (2019)
Neurophysiology NA Not reported NA NA Guo B , et al. (2019)
Repetitive behavior Self grooming: perseveration Refractory Grooming behavior assessments 2-3 months Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Restored Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion NA Not reported NA NA Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_CX546-1

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

Shank3 KO mice were subcutaneously injected with CX546 at 5mg per kg in 1% DMSO for 7 days.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Guo B , et al. (2019)
Neurophysiology NA Not reported NA NA Guo B , et al. (2019)
Repetitive behavior NA Not reported NA NA Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Refractory Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Refractory Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion NA Not reported NA NA Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_CX546-2

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

Shank3 KO mice were subcutaneously injected with CX546 at 10mg per kg in 1% DMSO for 7 days.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Guo B , et al. (2019)
Neurophysiology NA Not reported NA NA Guo B , et al. (2019)
Repetitive behavior NA Not reported NA NA Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Ameliorated Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion NA Not reported NA NA Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_CX546-3

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

Shank3 KO mice were subcutaneously injected with CX546 at 20 mg per kg in 1% DMSO for 7 days.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Guo B , et al. (2019)
Neurophysiology NA Not reported NA NA Guo B , et al. (2019)
Repetitive behavior NA Not reported NA NA Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Restored Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion NA Not reported NA NA Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_CX546-4

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous

Mutation:

400 micromolar CX546 was microinjected into the anterior cingulate cortex of the shank3 KO mice.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype NA Not reported NA NA Guo B , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Guo B , et al. (2019)
Neurophysiology NA Not reported NA NA Guo B , et al. (2019)
Repetitive behavior NA Not reported NA NA Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Ameliorated Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Restored Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion NA Not reported NA NA Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory NA Not reported NA NA Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_25_KO_HM_LPS

Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous; hemizygous

Mutation:

Shank3 mutant mice were treated with lipopolysaccharide (50microgram/kg, i.p., acute)

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: The Jackson Laboratory
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA ()
Circadian sleep/wake cycle NA Not reported NA NA ()
Motor phenotype NA Not reported NA NA ()
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA ()
Neurophysiology Neuronal activation Refractory Immunohistochemistry Adult ()
Repetitive behavior NA Not reported NA NA ()
Seizure NA Not reported NA NA ()
Sensory NA Not reported NA NA ()
Social behavior Social approach Refractory Three-chamber social approach test Adult ()
Communications NA Not reported NA NA ()
Developmental profile NA Not reported NA NA ()
Emotion NA Not reported NA NA ()
Immune response Cytokine levels: pro-inflammatory Side effect Elisa Adult, 2-5 months, 9-12 months ()
Learning & memory NA Not reported NA NA ()
Maternal behavior NA Not reported NA NA ()
Molecular profile NA Not reported NA NA ()

M_SHANK3_25_KO_HM_S1DZ-INACT

Model Type: RESCUE-Procedural
Model Genotype: Homozygous

Mutation:

Neural activity was optogenetically suppressed in the primary somatosensory cortex dysgranular zone in Shank3 mutant mice.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: The Jackson Laboratory
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA ()
Circadian sleep/wake cycle NA Not reported NA NA ()
Motor phenotype NA Not reported NA NA ()
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA ()
Neurophysiology NA Not reported NA NA ()
Repetitive behavior NA Not reported NA NA ()
Seizure NA Not reported NA NA ()
Sensory NA Not reported NA NA ()
Social behavior Social approach Refractory Three-chamber social approach test Adult ()
Communications NA Not reported NA NA ()
Developmental profile NA Not reported NA NA ()
Emotion NA Not reported NA NA ()
Immune response NA Not reported NA NA ()
Learning & memory NA Not reported NA NA ()
Maternal behavior NA Not reported NA NA ()
Molecular profile NA Not reported NA NA ()

M_SHANK3_26_CKO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Mice with selective knockout of Shank3 in the anterior cingulate cortex were generated CRISPR/Cas9 mediated genome editing by administering an AAV2/9 vector harboring Staphylococcus aureus Cas9 (SaCas9) and a single-guide RNA (sgRNA) to adult WT mice. This eliminated Shank3 alpha, beta and gamma isoforms in the anterior cingulate cortex.

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: -
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Guo B , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Guo B , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors No change Western blot 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: nmda receptors Increased Western blot 2-3 months Guo B , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: glutamate receptors: ampa receptors Decreased Western blot 2-3 months Guo B , et al. (2019)
Neurophysiology Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission Increased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Presynaptic function: paired-pulse facilitation No change Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current frequency: excitatory Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Miniature post synaptic current amplitude: excitatory Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Neurophysiology Epsp-spike relationship Decreased Whole-cell patch clamp 2-6 months Guo B , et al. (2019)
Repetitive behavior Self grooming: perseveration No change Grooming behavior assessments 2-3 months Guo B , et al. (2019)
Repetitive behavior Repetitive digging No change Marble-burying test 2-3 months Guo B , et al. (2019)
Seizure NA Not reported NA NA Guo B , et al. (2019)
Sensory NA Not reported NA NA Guo B , et al. (2019)
Social behavior Social interaction Decreased Reciprocal social interaction test 2-3 months Guo B , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 2-3 months Guo B , et al. (2019)
Communications NA Not reported NA NA Guo B , et al. (2019)
Developmental profile NA Not reported NA NA Guo B , et al. (2019)
Emotion Exploratory activity No change Novel object recognition test 2-3 months Guo B , et al. (2019)
Emotion Anxiety No change Elevated plus maze test 2-3 months Guo B , et al. (2019)
Emotion Anxiety No change Open field test 2-3 months Guo B , et al. (2019)
Immune response NA Not reported NA NA Guo B , et al. (2019)
Learning & memory Object recognition memory No change Novel object recognition test 2-3 months Guo B , et al. (2019)
Maternal behavior NA Not reported NA NA Guo B , et al. (2019)
Molecular profile Targeted expression Decreased Western blot 2-3 months Guo B , et al. (2019)
Molecular profile NA Not reported NA NA Guo B , et al. (2019)

M_SHANK3_27_CKO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Mice with selective ablation of Shank3 below cervical level 2 were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 with mice expressing Cre recombinase below cervical level 2 (Cdx2-Cre; Shank3f/+)

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: PMID 31398341; Akyol et al., 2008; Eric Fearon; RRID:IMSR_JAX:009350
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No change Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration No change Open field test 6-8 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Decreased Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Decreased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_28_CKO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Mice with selective ablation of Shank3 in all DRG, trigeminal, and sympathetic ganglia somatosensory neurons were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 with mice expressing Advillin-Cre recombinase (Advillin-Cre; Shank3f/+).

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: PMID 31398341; Hasegawa et al, 2007; Fan Wang; RRID:IMSR_JAX:032536
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No change Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Decreased Immunohistochemistry Adult Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the spinal cord: sensory and motor tracts Decreased Immunohistochemistry Adult Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event frequency: inhibitory currents Increased Whole-cell patch clamp Adult Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event frequency: excitatory currents No change Whole-cell patch clamp Adult Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event amplitude: inhibitory currents Decreased Whole-cell patch clamp Adult Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event amplitude: excitatory currents Decreased Whole-cell patch clamp Adult Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Decreased Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Decreased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_29_CKO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Mice with selective ablation of Shank3 in all DRG, trigeminal, and sympathetic ganglia somatosensory neurons were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 with mice expressing Advillin-Cre recombinase (Advillin-Cre; Shank3f/f).

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: PMID 31398341; Hasegawa et al, 2007; Fan Wang; RRID:IMSR_JAX:032536
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity Decreased Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No change Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the spinal cord: sensory and motor tracts Decreased Immunohistochemistry Adult Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration No change Open field test 6-8 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Decreased Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Decreased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile Targeted expression Decreased Immunohistochemistry 6-8 weeks Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_30_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain, the positive selection marker neo cassette is retained; M_SHANK3_4_KO_HT

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity Decreased Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No change Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the spinal cord: sensory and motor tracts Decreased Immunohistochemistry Adult Orefice LL , et al. (2019)
Neurophysiology Synaptic transmission Decreased Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Neurophysiology Intrinsic membrane properties Increased Whole-cell current clamp Adult Orefice LL , et al. (2019)
Neurophysiology Action potential property: firing rate Decreased Whole-cell voltage clamp Adult Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Decreased Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensory-evoked response: excitation: tactile stimulus No change Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile Size/growth Decreased Body weight measurement 3 weeks Orefice LL , et al. (2019)
Developmental profile Mortality/lethality No change Survival analysis 6 weeks Orefice LL , et al. (2019)
Developmental profile General characteristics Decreased Macroscopic analysis 3 weeks Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Decreased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity Decreased Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall Decreased Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_30_KO_HT_ISOGUVACINE-1

Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous

Mutation:

Mice with targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain are treated acutely with Isoguvacine, 2 mg/kg, i.p..

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype NA Not reported NA NA Orefice LL , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior NA Not reported NA NA Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No adverse effect Acoustic startle reflex test 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Response to air puff 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Social behavior NA Not reported NA NA Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion NA Not reported NA NA Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory NA Not reported NA NA Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_30_KO_HT_ISOGUVACINE-2

Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous

Mutation:

Mice with targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain are treated acutely with Isoguvacine, 10micromolar (=2mg/kg) s.c.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype NA Not reported NA NA Orefice LL , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior NA Not reported NA NA Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Sensory-evoked response: excitation: tactile stimulus Side effect Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Sensory NA Not reported NA NA Orefice LL , et al. (2019)
Social behavior NA Not reported NA NA Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion NA Not reported NA NA Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory NA Not reported NA NA Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_30_KO_HT_ISOGUVACINE-3

Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous

Mutation:

Mice with targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain are treated with chronic isoguvacine at 2mg/kg, i.p. from P1 to P42.

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity Restored Open field test 6 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No adverse effect Elevated plus maze test 6 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Restored Immunohistochemistry 6 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Refractory Immunohistochemistry 6 weeks Orefice LL , et al. (2019)
Neurophysiology Synaptic transmission Refractory Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Neurophysiology Synaptic transmission Restored Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event frequency: inhibitory currents Restored Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event frequency: excitatory currents Restored Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event amplitude: inhibitory currents Restored Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Neurophysiology Spontaneous post synaptic event amplitude: excitatory currents Restored Whole-cell patch clamp 6 weeks Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration Refractory Grooming behavior assessments 6 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Restored Marble-burying test 6 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Refractory Textured novel object recognition test (t-nort) 6 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No adverse effect Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Response to air puff 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating Refractory Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Social behavior Social memory Restored Three-chamber social approach test 6 weeks Orefice LL , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 6 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile Size/growth Restored Body weight measurement 3 weeks Orefice LL , et al. (2019)
Developmental profile Mortality/lethality No adverse effect Survival analysis 6 weeks Orefice LL , et al. (2019)
Developmental profile General characteristics Restored Macroscopic analysis 3 weeks Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Refractory Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No adverse effect Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Emotion Anxiety Restored Open field test 6 weeks Orefice LL , et al. (2019)
Emotion Anxiety Restored Elevated plus maze test 6 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall Refractory Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_31_KI_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase; M_SHANK3_21_KI_HM.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No change Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Decreased Immunohistochemistry Adult Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Decreased Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Decreased Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No change Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall Decreased Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_31_KI_HT-REINSTATEMENT-ADVILLIN

Model Type: RESCUE-Genetic
Model Genotype: Heterozygous

Mutation:

Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase. In the presence of Cre, the Shank3 PDZ domain is aligned into the proper orientation resulting in restored expression of most major SHANK3 isoforms. Parents mice were crossed with Advillin-Cre to restore Shank3 expression in all DRG, trigeminal, and sympathetic ganglia neurons.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No adverse effect Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No adverse effect Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Restored Immunohistochemistry Adult Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Refractory Immunohistochemistry Adult Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration Refractory Open field test 6-8 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Ameliorated Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Restored Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No adverse effect Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No adverse effect Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Ameliorated Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Ameliorated Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No adverse effect Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Restored Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Restored Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall Refractory Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_31_KI_HT-REINSTATEMENT-CDX2

Model Type: RESCUE-Genetic
Model Genotype: Heterozygous

Mutation:

Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase. In the presence of Cre, the Shank3 PDZ domain is aligned into the proper orientation resulting in restored expression of most major SHANK3 isoforms. Parents mice were crossed with Cdx2-Cre to restore Shank3 expression in cells below cervical level 2.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No adverse effect Open field test 6-8 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No adverse effect Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Neuroanatomy / ultrastructure / cytoarchitecture NA Not reported NA NA Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Self grooming: perseveration Refractory Open field test 6-8 weeks Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Ameliorated Marble-burying test 6-8 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Restored Textured novel object recognition test (t-nort) 6-8 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No adverse effect Acoustic startle reflex test 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Response to air puff 6-8 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No adverse effect Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social memory Ameliorated Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 6-8 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Ameliorated Prepulse inhibition 6-8 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No adverse effect Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Restored Elevated plus maze test 6-8 weeks Orefice LL , et al. (2019)
Emotion Anxiety Restored Open field test 6-8 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall Refractory Novel object recognition test 6-8 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_31_KI_HT_REINSTATEMENT-ADVILLIN-ICRE-1

Model Type: RESCUE-Genetic
Model Genotype: Heterozygous

Mutation:

Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^FX/+) in the postnatal period (P28-P33) were generated by crossing mice possessing cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase, and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No adverse effect Open field test 6 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No adverse effect Elevated plus maze test 6 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Refractory Marble-burying test 6 weeks Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Refractory Marble-burying test 6 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Refractory Textured novel object recognition test (t-nort) 6 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No adverse effect Acoustic startle reflex test 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Restored Response to air puff 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No adverse effect Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Social behavior Social memory Refractory Three-chamber social approach test 6 weeks Orefice LL , et al. (2019)
Social behavior Social approach Restored Three-chamber social approach test 6 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Refractory Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No adverse effect Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Emotion Anxiety Refractory Elevated plus maze test 6 weeks Orefice LL , et al. (2019)
Emotion Anxiety Refractory Open field test 6 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall Refractory Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_32_KI_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase, Shank3^FX/FX; M_SHANK3_21_KI_HM.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype NA Not reported NA NA Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the spinal cord: sensory and motor tracts Decreased Immunohistochemistry 6-8 weeks Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior NA Not reported NA NA Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory NA Not reported NA NA Orefice LL , et al. (2019)
Social behavior NA Not reported NA NA Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion NA Not reported NA NA Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory NA Not reported NA NA Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile Targeted expression Decreased Immunohistochemistry 6-8 weeks Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_32_KI_HM-REINSTATEMENT-ADVILLIN

Model Type: RESCUE-Genetic
Model Genotype: Homozygous

Mutation:

Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase. In the presence of Cre, the Shank3 PDZ domain is aligned into the proper orientation resulting in restored expression of most major SHANK3 isoforms. Parents mice were crossed with Advillin-Cre to restore Shank3 expression in all DRG, trigeminal, and sympathetic ganglia neurons; Advillin-Cre; Shank3^FX/FX.

Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype NA Not reported NA NA Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the spinal cord: sensory and motor tracts Restored Immunohistochemistry 6-8 weeks Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior NA Not reported NA NA Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory NA Not reported NA NA Orefice LL , et al. (2019)
Social behavior NA Not reported NA NA Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion NA Not reported NA NA Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory NA Not reported NA NA Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile Targeted expression Restored Immunohistochemistry 6-8 weeks Orefice LL , et al. (2019)
Molecular profile Targeted expression Restored Immunohistochemistry NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_33_KO_HM

Model Type: Genetic
Model Genotype: Homozygous

Mutation:

Targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain, the positive selection marker neo cassette is retained; M_SHANK3_4_KO_HT

Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J

Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype NA Not reported NA NA Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuroreceptor levels: gaba-r: gabaa No change Immunohistochemistry Adult Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Morphology of the spinal cord: sensory and motor tracts Decreased Immunohistochemistry Adult Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior NA Not reported NA NA Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory NA Not reported NA NA Orefice LL , et al. (2019)
Social behavior NA Not reported NA NA Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion NA Not reported NA NA Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory NA Not reported NA NA Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile Targeted expression Decreased Western blot 6-8 weeks Orefice LL , et al. (2019)
Molecular profile Targeted expression Decreased Immunohistochemistry 6-8 weeks Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_34_CKO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^f/+) in the early postnatal period (P5) were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratory; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
Category Entity Value Change Experimental Paradigm Age at Testing Author, Year
Physiological parameters NA Not reported NA NA Orefice LL , et al. (2019)
Circadian sleep/wake cycle NA Not reported NA NA Orefice LL , et al. (2019)
Motor phenotype General locomotor activity: ambulatory activity No change Open field test 6 weeks Orefice LL , et al. (2019)
Motor phenotype General locomotor activity No change Elevated plus maze test 6 weeks Orefice LL , et al. (2019)
Neuroanatomy / Ultrastructure / Cytoarchitecture Neuronal number: interneurons Abnormal Immunohistochemistry 6 weeks Orefice LL , et al. (2019)
Neurophysiology NA Not reported NA NA Orefice LL , et al. (2019)
Repetitive behavior Repetitive digging Decreased Marble-burying test 6 weeks Orefice LL , et al. (2019)
Seizure NA Not reported NA NA Orefice LL , et al. (2019)
Sensory Tactile memory Decreased Textured novel object recognition test (t-nort) 6 weeks Orefice LL , et al. (2019)
Sensory Startle response: acoustic stimulus No change Acoustic startle reflex test 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Response to air puff 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating: tactile cue Increased Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Sensory Sensorimotor gating No change Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Social behavior Social memory Decreased Three-chamber social approach test 6 weeks Orefice LL , et al. (2019)
Social behavior Social approach Decreased Three-chamber social approach test 6 weeks Orefice LL , et al. (2019)
Communications NA Not reported NA NA Orefice LL , et al. (2019)
Developmental profile NA Not reported NA NA Orefice LL , et al. (2019)
Emotion Habituation to aversive stimuli Decreased Prepulse inhibition 6 weeks Orefice LL , et al. (2019)
Emotion Exploratory activity No change Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Elevated plus maze test 6 weeks Orefice LL , et al. (2019)
Emotion Anxiety Increased Open field test 6 weeks Orefice LL , et al. (2019)
Immune response NA Not reported NA NA Orefice LL , et al. (2019)
Learning & memory Object recognition memory: long-term recall No change Novel object recognition test 6 weeks Orefice LL , et al. (2019)
Maternal behavior NA Not reported NA NA Orefice LL , et al. (2019)
Molecular profile NA Not reported NA NA Orefice LL , et al. (2019)

M_SHANK3_35_CKO_HT

Model Type: Genetic
Model Genotype: Heterozygous

Mutation:

Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^f/+) in the early postnatal period (P10) were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.

Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1

Es cell line: -
Model Source: Jackson Laboratory; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
Category