SHANK3 SH3 and multiple ankyrin repeat domains 3
Model Species
Mus musculusGene Aliases
OTTMUSP00000028933; SH3 and multiple ankyrin repeat domains protein 3; SPANK-2; proSAP2; proline-rich synapse-associated protein 2Models / Rescue Models
107 / 46Rescue Model
-Model Summary
Specific deficits in synaptic function and plasticity, along with reduced reciprocal social interactions in Shank3 heterozygous mice.
External Links
Reports related to SHANK3 (40 Reports)
| # | Type | Title | Author, Year |
|---|---|---|---|
| 1 | Primary | Haploinsufficiency of the autism-associated Shank3 gene leads to deficits in synaptic function, social interaction, and social communication. | Bozdagi O , et al. (2010) |
| 2 | Additional | Shank3 mutant mice display autistic-like behaviours and striatal dysfunction. | Pea J , et al. (2011) |
| 3 | Additional | Synaptic dysfunction and abnormal behaviors in mice lacking major isoforms of Shank3. | Wang X , et al. (2011) |
| 4 | Additional | Enhanced polyubiquitination of Shank3 and NMDA receptor in a mouse model of autism. | Bangash MA , et al. (2011) |
| 5 | Additional | Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2. | Schmeisser MJ , et al. (2012) |
| 6 | Additional | Insulin-like growth factor-1 rescues synaptic and motor deficits in a mouse model of autism and developmental delay. | Bozdagi O , et al. (2013) |
| 7 | Additional | Loss of predominant Shank3 isoforms results in hippocampus-dependent impairments in behavior and synaptic transmission. | Kouser M , et al. (2013) |
| 8 | Additional | Autism-like Deficits in Shank3-Deficient Mice Are Rescued by Targeting Actin Regulators. | Duffney LJ , et al. (2015) |
| 9 | Additional | Autism-Associated Insertion Mutation (InsG) of Shank3 Exon 21 Causes Impaired Synaptic Transmission and Behavioral Deficits. | Speed HE , et al. (2015) |
| 10 | Additional | Cerebellar associative sensory learning defects in five mouse autism models. | Kloth AD , et al. (2015) |
| 11 | Additional | Altered Striatal Synaptic Function and Abnormal Behaviour in Shank3 Exon4-9 Deletion Mouse Model of Autism. | Jaramillo TC , et al. (2015) |
| 12 | Additional | Mice with Shank3 Mutations Associated with ASD and Schizophrenia Display Both Shared and Distinct Defects. | Zhou Y , et al. (2015) |
| 13 | Additional | CLK2 inhibition ameliorates autistic features associated with SHANK3 deficiency. | Bidinosti M , et al. (2016) |
| 14 | Additional | Adult restoration of Shank3 expression rescues selective autistic-like phenotypes. | Mei Y , et al. (2016) |
| 15 | Additional | Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. | Wang X , et al. (2016) |
| 16 | Additional | Peripheral Mechanosensory Neuron Dysfunction Underlies Tactile and Behavioral Deficits in Mouse Models of ASDs. | Orefice LL , et al. (2016) |
| 17 | Additional | Novel Shank3 mutant exhibits behaviors with face validity for autism and altered striatal and hippocampal function. | Jaramillo TC , et al. (2016) |
| 18 | Additional | Comprehensive analysis of two Shank3 and the Cacna1c mouse models of autism spectrum disorder. | Kabitzke PA , et al. (2017) |
| 19 | Additional | Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition. | Qin L , et al. (2018) |
| 20 | Additional | Dysbiosis of microbiome and probiotic treatment in a genetic model of autism spectrum disorders. | Tabouy L , et al. (2018) |
| 21 | Additional | Heterogeneity of Cell Surface Glutamate and GABA Receptor Expression in Shank and CNTN4 Autism Mouse Models. | Heise C , et al. (2018) |
| 22 | Additional | Dietary Zinc Supplementation Prevents Autism Related Behaviors and Striatal Synaptic Dysfunction in Shank3 Exon 13-16 Mutant Mice. | Fourie C , et al. (2018) |
| 23 | Additional | Mechanisms Underlying Microbial-Mediated Changes in Social Behavior in Mouse Models of Autism Spectrum Disorder. | Sgritta M , et al. (2018) |
| 24 | Additional | An autism-linked missense mutation in SHANK3 reveals the modularity of Shank3 function. | Wang L , et al. (2019) |
| 25 | Additional | Striatal Inhibition of MeCP2 or TSC1 Produces Sociability Deficits and Repetitive Behaviors. | Lee Y , et al. (2019) |
| 26 | Additional | Hypothesis-driven investigations of diverse pharmacological targets in two mouse models of autism. | Rhine MA , et al. (2019) |
| 27 | Additional | Shank3 modulates sleep and expression of circadian transcription factors. | Ingiosi AM , et al. (2019) |
| 28 | Additional | Anterior cingulate cortex dysfunction underlies social deficits in Shank3 mutant mice. | Guo B , et al. (2019) |
| 29 | Additional | Targeting Peripheral Somatosensory Neurons to Improve Tactile-Related Phenotypes in ASD Models. | Orefice LL , et al. (2019) |
| 30 | Additional | IL-17a Promotes Sociability in Mouse Models of Neurodevelopmental Disorders </title> | () |
| 31 | Additional | Tau Reduction Prevents Key Features of Autism in Mouse Models | Tai C et al. (2020) |
| 32 | Additional | Autism-Associated Shank3 Is Essential for Homeostatic Compensation in Rodent V1 | Tatavarty V et al. (2020) |
| 33 | Additional | Autism-associated SHANK3 mutations impair maturation of neuromuscular junctions and striated muscles | Lutz AK et al. (2020) |
| 34 | Additional | A chimeric mouse model to study human iPSC-derived neurons: the case of a truncating SHANK3 mutation | Vitrac A et al. (2020) |
| 35 | Additional | Major motor and gait deficits with sexual dimorphism in a Shank3 mutant mouse model | Matas E et al. (2021) |
| 36 | Additional | Inhibition of Trpv4 rescues circuit and social deficits unmasked by acute inflammatory response in a Shank3 mouse model of Autism | Tzanoulinou S et al. (2022) |
| 37 | Additional | Disrupted social memory ensembles in the ventral hippocampus underlie social amnesia in autism-associated Shank3 mutant mice | Tao K et al. (2022) |
| 38 | Additional | Absence of familiarity triggers hallmarks of autism in mouse model through aberrant tail-of-striatum and prelimbic cortex signaling | Krüttner S et al. (2022) |
| 39 | Additional | SHANK3 deficiency leads to myelin defects in the central and peripheral nervous system | Malara M et al. (2022) |
| 40 | Additional | - | Tripathi MK et al. (2023) |
Show all Construct Details
Show all Phenotypic Profiles
M_SHANK3_10_KI_HM_INSG
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Autism-associated guanine nucleotide insertion mutation (Shank3G) present in exon 21 was inserted into the Shank 3 gene, with the construct containing the mutation within exon21 sequence knocked into intron 20 .The additional G results in a frameshift mutation and a premature stop codon downstream of exon 21. Mice containing the Shank3G mutation were crossed for 4 generations with C57BL6J. Het and homozygous mice were generated as littermates.
Allele Type: ASD LOF mutation
Strain of Origin: 129Sv/Evs6
Genetic Background: C57BL/6J*129Sv/Evs6
Es cell line: SM-1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | 2-6 months | Speed HE , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic neuroreceptors | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic transmission | Decreased | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic plasticity: post-tetanic potentiation (ptp) | Decreased | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | No change | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | Decreased | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Neurophysiology | Decay kinetics of evoked post synaptic currents | No change | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 2-6 months | Speed HE , et al. (2015) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 2-6 months | Speed HE , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Sensory | Vision | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Social behavior | Social memory | No change | Three-chamber social approach test | 2-6 months | Speed HE , et al. (2015) |
| Social behavior | Social approach | No change | Three-chamber social approach test | 2-6 months | Speed HE , et al. (2015) |
| Social behavior | Nest building behavior | Decreased | Nest building assay | 2-6 months | Speed HE , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Developmental profile | Size/growth | No change | Body weight measurement | NA | Speed HE , et al. (2015) |
| Emotion | Thigmotaxis | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Emotion | Response to novelty | Decreased | Open field test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Response to novelty | Decreased | Light-dark exploration test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Response to novelty | Decreased | Elevated plus maze test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Anxiety | No change | Open field test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Anxiety | No change | Light-dark exploration test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Anxiety | No change | Elevated plus maze test | 5-6 months | Speed HE , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Learning & memory | Spatial learning | Decreased | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Molecular profile | Protein phosphorylation | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Molecular profile | Protein phosphorylation | Decreased | Western blot | 5-6 months | Speed HE , et al. (2015) |
M_SHANK3_10_KI_HM_INSG_REINSTATE-SHANK3
Model Type: RESCUE-Genetic
Model Genotype: Homozygous
Mutation:
Genetic reinstatement of wild type Shank3 as the Shank3G cassette containing knockin mice generated in the previous steps were crossed with CreESRT transgenic mice (B6.Cg-Tg(CAG-cre/Esr1)5Amc/J, activated by Tamoxifen administration to the mice, to excise the insertion reversing the mutation.
Allele Type: ASD LOF mutation
Strain of Origin: 129Sv/Evs6
Genetic Background: C57BL/6J*129Sv/Evs6*B6
Es cell line: SM-1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Motor phenotype | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Neurophysiology | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Repetitive behavior | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Social behavior | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Emotion | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Molecular profile | Targeted expression | Restored | Western blot | 3 months | Speed HE , et al. (2015) |
M_SHANK3_10_KI_HT_INSG
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Autism-associated guanine nucleotide insertion mutation (Shank3G) present in exon 21 was inserted into the Shank 3 gene, with the construct containing the mutation within exon21 sequence knocked into intron 20 .The additional G results in a frameshift mutation and a premature stop codon downstream of exon 21. Mice containing the Shank3G mutation were crossed for 4 generations with C57BL6J. Het and homozygous mice were generated as littermates.
Allele Type: ASD LOF mutation
Strain of Origin: 129Sv/Evs6
Genetic Background: C57BL/6J*129Sv/Evs6
Es cell line: SM-1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 2-6 months | Speed HE , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic neuroreceptors | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic transmission | Decreased | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | No change | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | Decreased | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Neurophysiology | Presynaptic function: presynaptic fiber volley | No change | Field potential recordings | NA | Speed HE , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Neurophysiology | Decay kinetics of evoked post synaptic currents | No change | Whole-cell patch clamp | NA | Speed HE , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 2-6 months | Speed HE , et al. (2015) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | 2-6 months | Speed HE , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Sensory | Vision | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Social behavior | Social memory | No change | Three-chamber social approach test | 2-6 months | Speed HE , et al. (2015) |
| Social behavior | Social approach | No change | Three-chamber social approach test | 2-6 months | Speed HE , et al. (2015) |
| Social behavior | Nest building behavior | Decreased | Nest building assay | 2-6 months | Speed HE , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Developmental profile | Size/growth | No change | Body weight measurement | NA | Speed HE , et al. (2015) |
| Emotion | Thigmotaxis | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Emotion | Response to novelty | No change | Open field test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Response to novelty | No change | Light-dark exploration test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Response to novelty | No change | Elevated plus maze test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Anxiety | No change | Open field test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Anxiety | No change | Light-dark exploration test | 5-6 months | Speed HE , et al. (2015) |
| Emotion | Anxiety | No change | Elevated plus maze test | 5-6 months | Speed HE , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Learning & memory | Spatial learning | No change | Morris water maze test | 2-6 months | Speed HE , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Speed HE , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 5-6 months | Speed HE , et al. (2015) |
| Molecular profile | Protein phosphorylation | No change | Western blot | 5-6 months | Speed HE , et al. (2015) |
M_SHANK3_13_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted Cre-LoxP mediated excision of exons 4-9 of Shank3 gene containing the ankyrin repeats. After germline transmission in C57BL6J, knock in mice were mated with mice expressing Flp1 recombinase to excise the Neo cassette used for positive selection. The resulting conditional Shank3 mice were crossed with mice expressing ZP3-Cre to excise exon4-9 in oocytes of female pups, then further crossed with C57BL6J, to homozygosity (Authors do not note that the construct used or the final mice are the same as that reported used in Bozdagi et al Mol Autism (2010) or Yang et al J Neurosci (2012), even if exon 4-9 are targeted in all these studies, therefore they are presumed to be different here).
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | NA | Jaramillo TC , et al. (2015) |
| Motor phenotype | General locomotor activity | No change | Open field test | NA | Jaramillo TC , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neurophysiology | Synaptic transmission: excitatory | No change | Field potential recordings | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Decreased | Field potential recordings | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | No change | Field potential recordings | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Paired-pulse ratio | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 3-5 months | Jaramillo TC , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Sensory | Tactile memory | No change | Textured novel object recognition test (t-nort) | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | No change | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | No change | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Vision | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 3-5 months | Jaramillo TC , et al. (2015) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Social behavior | Social memory | No change | Reciprocal social interaction test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social interaction: with juveniles | No change | Reciprocal social interaction test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social interaction | No change | Partition test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Nest building behavior | No change | Nest building assay | 3-5 months | Jaramillo TC , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | Ultrasonic vocalization: isolation induced | Increased | Monitoring ultrasonic vocalizations | P4, p6 | Jaramillo TC , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Emotion | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Thigmotaxis | Increased | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Open field test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Marble-burying test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Light-dark exploration test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Elevated plus maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Learning & memory | Object recognition memory: long-term recall | Decreased | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Spatial reference memory | Decreased | Object-place recognition test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Spatial learning | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Object recognition memory | Decreased | Object-place recognition test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Cued or contextual fear conditioning | No change | Fear conditioning test | NA | Jaramillo TC , et al. (2015) |
| Learning & memory | Cognitive flexibility | No change | Morris water maze test | NA | Jaramillo TC , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Western blot | 3-6 months | Jaramillo TC , et al. (2015) |
| Molecular profile | Protein localization: synapse | No change | Western blot | 3-6 months | Jaramillo TC , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | 3-6 months | Jaramillo TC , et al. (2015) |
M_SHANK3_13_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Targeted Cre-LoxP mediated excision of exons 4-9 of Shank3 gene containing the ankyrin repeats. After germline transmission in C57BL6J, knock in mice were mated with mice expressing Flp1 recombinase to excise the Neo cassette used for positive selection. The resulting conditional Shank3 mice were crossed with mice expressing ZP3-Cre to excise exon4-9 in oocytes of female pups, then further crossed with C57BL6J, to homozygosity (Authors do not note that the construct used or the final mice are the same as that reported used in Bozdagi et al Mol Autism (2010) or Yang et al J Neurosci (2012), even if exon 4-9 are targeted in all these studies, therefore they are presumed to be different here).
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 3-5 months | Jaramillo TC , et al. (2015) |
| Motor phenotype | General locomotor activity | No change | Open field test | 3-5 months | Jaramillo TC , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | No change | Field potential recordings | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | No change | Field potential recordings | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | No change | Field potential recordings | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Paired-pulse ratio | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P14-p17 | Jaramillo TC , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 3-4 weeks | Jaramillo TC , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 3-5 months | Jaramillo TC , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Sensory | Vision | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 3-5 months | Jaramillo TC , et al. (2015) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social memory | No change | Reciprocal social interaction test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social interaction: with juveniles | No change | Reciprocal social interaction test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social interaction | No change | Reciprocal social interaction test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Social interaction | No change | Partition test | 3-5 months | Jaramillo TC , et al. (2015) |
| Social behavior | Nest building behavior | No change | Nest building assay | 3-5 months | Jaramillo TC , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Communications | Ultrasonic vocalization: isolation induced | Increased | Monitoring ultrasonic vocalizations | P4, p12 | Jaramillo TC , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Emotion | Thigmotaxis | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Open field test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Marble-burying test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Light-dark exploration test | 3-5 months | Jaramillo TC , et al. (2015) |
| Emotion | Anxiety | No change | Elevated plus maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Spatial reference memory | Decreased | Object-place recognition test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Spatial learning | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Object recognition memory | Decreased | Object-place recognition test | 3-5 months | Jaramillo TC , et al. (2015) |
| Learning & memory | Cued or contextual fear conditioning | No change | Fear conditioning test | NA | Jaramillo TC , et al. (2015) |
| Learning & memory | Cognitive flexibility | No change | Morris water maze test | 3-5 months | Jaramillo TC , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Jaramillo TC , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 3-6 months | Jaramillo TC , et al. (2015) |
| Molecular profile | Protein localization: synapse | No change | Western blot | 3-6 months | Jaramillo TC , et al. (2015) |
M_SHANK3_15_KI_HM_INSG
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Autism-associated guanine nucleotide insertion mutation (Shank3InsG) was placed at position 3680 in exon 21, this insertion causes a frameshift and a stop codon immediately after the G insertion. The mice were maintained in C57B6/S129Sv background. Mice were bred to homozygosity normally.
Allele Type: ASD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv
Es cell line: R1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | Adult | Zhou Y , et al. (2015) |
| Motor phenotype | General locomotor activity | Decreased | Open field test | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Increased | Western blot | P14 | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-cox staining | 2 months | Zhou Y , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Presynaptic function | No change | Field potential recordings | P14, adult | Zhou Y , et al. (2015) |
| Neurophysiology | Population spikes | Decreased | Field potential recordings | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Population spikes | Decreased | Field potential recordings | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic currents: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Increased | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | Adult | Zhou Y , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | Decreased | Acoustic startle reflex test | Adult | Zhou Y , et al. (2015) |
| Sensory | Sensorimotor gating | Decreased | Prepulse inhibition | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social dominance | Increased | Tube test of social dominance | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | Adult | Zhou Y , et al. (2015) |
| Social behavior | Juvenile play | Decreased | Reciprocal social interaction test | 3 weeks | Zhou Y , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | P2-p12 | Zhou Y , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity: habituation | No change | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity | Decreased | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Anxiety | Increased | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Anxiety | Increased | Elevated zero maze test | Adult | Zhou Y , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Learning & memory | Spatial working memory | No change | T-maze test | Adult | Zhou Y , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Quantitative pcr (qrt-pcr) | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | P14 | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Increased | Western blot | P14 | Zhou Y , et al. (2015) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Gene expression | Increased | Quantitative pcr (qrt-pcr) | 2 months | Zhou Y , et al. (2015) |
M_SHANK3_15_KI_HT_INSG
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Autism-associated guanine nucleotide insertion mutation (Shank3InsG) was placed at position 3680 in exon 21, this insertion causes a frameshift and a stop codon immediately after the G insertion. The mice were maintained in C57B6/S129Sv background. Mice were bred to homozygosity.
Allele Type: ASD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv
Es cell line: R1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | Adult | Zhou Y , et al. (2015) |
| Motor phenotype | General locomotor activity | Decreased | Open field test | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | No change | Golgi-cox staining | 2 months | Zhou Y , et al. (2015) |
| Neurophysiology | Presynaptic function | No change | Field potential recordings | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Population spikes | No change | Field potential recordings | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 5 months | Zhou Y , et al. (2015) |
| Repetitive behavior | Allogrooming: perseveration | No change | Grooming behavior assessments | Adult | Zhou Y , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | Adult | Zhou Y , et al. (2015) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social dominance | No change | Tube test of social dominance | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social approach | No change | Three-chamber social approach test | Adult | Zhou Y , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity: habituation | No change | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity | Decreased | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Anxiety | Increased | Elevated zero maze test | Adult | Zhou Y , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
M_SHANK3_17_KI_HM_R1117X
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Schizophrenia associated non-sense mutation changing arginine to a stop codon (R1117X) was introduced by changing the codon for R 'CGG' to stop codon TGA in position 1117. Mice were maintained in C57Bl6*129Sv background and bred to homozygosity.
Allele Type: NDD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv
Es cell line: R1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | Adult | Zhou Y , et al. (2015) |
| Motor phenotype | General locomotor activity | Decreased | Open field test | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-cox staining | 2 months | Zhou Y , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Presynaptic function | No change | Field potential recordings | P14, adult | Zhou Y , et al. (2015) |
| Neurophysiology | Population spikes | Decreased | Field potential recordings | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Population spikes | No change | Field potential recordings | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic currents: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Increased | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P14 | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 5 months | Zhou Y , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | Decreased | Acoustic startle reflex test | Adult | Zhou Y , et al. (2015) |
| Sensory | Sensorimotor gating | Decreased | Prepulse inhibition | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social dominance | Increased | Tube test of social dominance | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | Adult | Zhou Y , et al. (2015) |
| Social behavior | Juvenile play | No change | Reciprocal social interaction test | 3 weeks | Zhou Y , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | P2-p12 | Zhou Y , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity: habituation | No change | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity | Decreased | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Anxiety | Increased | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Anxiety | Increased | Elevated zero maze test | Adult | Zhou Y , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Learning & memory | Spatial working memory | No change | T-maze test | Adult | Zhou Y , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Western blot | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Quantitative pcr (qrt-pcr) | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | P14 | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | No change | Western blot | P14 | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | P14 | Zhou Y , et al. (2015) |
| Molecular profile | Protein expression level evidence | No change | Western blot | 2 months | Zhou Y , et al. (2015) |
| Molecular profile | Gene expression | No change | Quantitative pcr (qrt-pcr) | 2 months | Zhou Y , et al. (2015) |
M_SHANK3_17_KI_HT_R1117X
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Schizophrenia associated non-sense mutation changing arginine to a stop codon (R1117X) was introduced by changing the codon for R 'CGG' to stop codon TGA in position 1117. Mice were maintained in C57Bl6*129Sv background.
Allele Type: NDD LOF mutation
Strain of Origin: -
Genetic Background: C57BL/6J*S129Sv
Es cell line: R1
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | Adult | Zhou Y , et al. (2015) |
| Motor phenotype | General locomotor activity | No change | Open field test | Adult | Zhou Y , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-cox staining | 2 months | Zhou Y , et al. (2015) |
| Neurophysiology | Presynaptic function | No change | Field potential recordings | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Population spikes | No change | Field potential recordings | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | Adult | Zhou Y , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | Adult | Zhou Y , et al. (2015) |
| Repetitive behavior | Allogrooming: perseveration | Increased | Grooming behavior assessments | Adult | Zhou Y , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | Adult | Zhou Y , et al. (2015) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social dominance | Increased | Tube test of social dominance | Adult | Zhou Y , et al. (2015) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | Adult | Zhou Y , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity: habituation | No change | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Exploratory activity | No change | Open field test | Adult | Zhou Y , et al. (2015) |
| Emotion | Anxiety | Increased | Elevated zero maze test | Adult | Zhou Y , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Zhou Y , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | Adult | Zhou Y , et al. (2015) |
M_SHANK3_19_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Disruption of all murine Shank3 isoforms by cre/loxP mediated excision of exons 4-22 of the Shank3 gene, spanning 58 kb to include the coding sequences for all known Shank3 isoforms. The floxed mice were bred with CMV-cre mice to generate mice with deletions and bred till homozygosity. Authors report loss of all known Shank3 mRNA and protein.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Category | Signaling: nitric oxide pathway | Increased | Griess reagent assay | unreported | Tripathi MK et al. (2023) |
| Category | Signaling: nitric oxide pathway | Increased | Cyclic nucleotide activity assay | unreported | Tripathi MK et al. (2023) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | unreported | Tripathi MK et al. (2023) |
| Motor phenotype | Swimming ability | No change | Morris water maze test | Adult | Wang X , et al. (2016) |
| Motor phenotype | Self grooming: artificial stress evoked | Increased | Grooming behavior assessments | 4-7 months | Wang X , et al. (2016) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | >2 months | Wang X , et al. (2016) |
| Motor phenotype | Grip strength | No change | Grip strength test | NA | Wang X , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Home cage behavior | Adult | Wang X , et al. (2016) |
| Motor phenotype | General locomotor activity | Decreased | Open field test | Adult | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synapse density | Decreased | Western blot | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: inhibitory neurons | Decreased | Immunofluorescence staining | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: excitatory neurons | Decreased | Immunofluorescence staining | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-Cox staining | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Thalamic morphology | Increased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size | Decreased | Electron microscopy | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size | No change | Electron microscopy | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Olfactory bulb morphology | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: mglur | Increased | Western blot | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: mglur | Increased | Immunostaining | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the basal ganglia: striatum: caudoputamen | Increased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the basal ganglia: lentiform nucleus: globus pallidus | Increased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the amygdala | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the optic tract | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of stria terminalis | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of fornix | No change | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of fimbria | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of anterior commissure | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Hippocampal morphology | Decreased | Voxel based morphometry | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dti: fractional anisotropy or relative anisotropy in brain regions | Decreased | Diffusion tensor imaging (dti) | 2-2.5 months | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Golgi-cox staining | 8 weeks | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | No change | Golgi-cox staining | 8 weeks | Wang X , et al. (2016) |
| Neurophysiology | Oxidative stress in neurons | Increased | Western blot | unreported | Tripathi MK et al. (2023) |
| Neurophysiology | Synaptic plasticity: striatal ltd | Decreased | Whole-cell patch clamp | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Spontaneous post synaptic event frequency: excitatory currents | Decreased | Whole-cell patch clamp | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Spontaneous post synaptic event amplitude: excitatory currents | No change | Whole-cell patch clamp | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Presynaptic function | Decreased | High-frequency stimulation (hfs) | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Intrinsic membrane properties | No change | Whole-cell patch clamp | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Event related oscillations (eros) in electroencephalography (eeg): coherence | Decreased | In vivo local field potential (lfp) recordings | 3-4 months | Wang X , et al. (2016) |
| Neurophysiology | Event related oscillations (eros) in electroencephalography (eeg) | Decreased | In vivo local field potential (lfp) recordings | 3-4 months | Wang X , et al. (2016) |
| Neurophysiology | Action potential property: firing rate | Increased | Whole-cell patch clamp | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Action potential property: firing rate | Decreased | In vivo local field potential (lfp) recordings | 3-4 months | Wang X , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 4-7 months | Wang X , et al. (2016) |
| Repetitive behavior | Repetitive nose pokes | No change | Hole-board test | 4-7 months | Wang X , et al. (2016) |
| Seizure | Seizures | No change | Observation of seizures | Adult | Wang X , et al. (2016) |
| Sensory | Vision | No change | Morris water maze test | Adult | Wang X , et al. (2016) |
| Sensory | Startle response: acoustic stimulus | Decreased | Acoustic startle reflex test | Adult | Wang X , et al. (2016) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Wang X , et al. (2016) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | unreported | Tripathi MK et al. (2023) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | unreported | Tripathi MK et al. (2023) |
| Social behavior | Social scent marking or recognition | Decreased | Homing test | P15 | Wang X , et al. (2016) |
| Social behavior | Social interaction | No change | Reciprocal social interaction test | >2 months | Wang X , et al. (2016) |
| Social behavior | Social dominance | Decreased | Dominance hierarchy formation | >2 months | Wang X , et al. (2016) |
| Social behavior | Social approach | No change | Three-chamber social approach test | >2 months | Wang X , et al. (2016) |
| Social behavior | Self grooming: social context | Increased | Grooming behavior assessments | >2 months | Wang X , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Communications | Ultrasonic vocalization: isolation induced | Decreased | Monitoring ultrasonic vocalizations | P4 | Wang X , et al. (2016) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Decreased | Monitoring ultrasonic vocalizations | Adult | Wang X , et al. (2016) |
| Developmental profile | Size/growth | No change | Body weight measurement | P21, 3-6 months | Wang X , et al. (2016) |
| Developmental profile | Mortality/lethality | No change | General observations | Adult | Wang X , et al. (2016) |
| Developmental profile | Developmental trajectory | Decreased | General observations | P4 | Wang X , et al. (2016) |
| Emotion | Anxiety | Increased | Elevated plus maze test | unreported | Tripathi MK et al. (2023) |
| Emotion | Exploratory activity | Decreased | Novel cage test | Adult | Wang X , et al. (2016) |
| Emotion | Exploratory activity | Decreased | Home cage behavior | Adult | Wang X , et al. (2016) |
| Emotion | Anxiety | Increased | Light-dark exploration test | Adult | Wang X , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Learning & memory | Object recognition memory | Decreased | Novel object recognition test | unreported | Tripathi MK et al. (2023) |
| Learning & memory | Object recognition memory | No change | Novel object recognition test | unreported | Tripathi MK et al. (2023) |
| Learning & memory | Spatial reference memory | Decreased | Morris water maze test | Adult | Wang X , et al. (2016) |
| Learning & memory | Spatial learning | No change | Morris water maze test | Adult | Wang X , et al. (2016) |
| Learning & memory | Reward reinforced choice behavior | Decreased | Operant conditioning paradigm | Adult | Wang X , et al. (2016) |
| Learning & memory | Olfactory learning and memory | No change | Olfactory habituation-dishabituation test | 2 months | Wang X , et al. (2016) |
| Learning & memory | Cued or contextual fear conditioning: memory of cue | No change | Fear conditioning test | Adult | Wang X , et al. (2016) |
| Learning & memory | Cued or contextual fear conditioning: memory of context | No change | Fear conditioning test | Adult | Wang X , et al. (2016) |
| Learning & memory | Cognitive flexibility | Decreased | Morris water maze test | Adult | Wang X , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Molecular profile | Targeted expression | Decreased | Western blot | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Signaling: PKA pathway | No change | Cyclic nucleotide activity assay | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Signaling: nitric oxide pathway | Increased | Griess reagent assay | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Signaling: nitric oxide pathway | Increased | Cyclic nucleotide activity assay | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Protein phosphorylation | Increased | Western blot | 8 weeks | Wang X , et al. (2016) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | 8 weeks | Wang X , et al. (2016) |
| Molecular profile | Gene expression | No change | Quantitative pcr (qrt-pcr) | 8 weeks | Wang X , et al. (2016) |
M_SHANK3_19_KO_HM_7-NITROINDAZOLE
Model Type: RESCUE-Pharmacological
Model Genotype: Homozygous
Mutation:
Shank3 knockout (MGI:6162240) mice were treated with neuronal nitric oxide synthase (nNOS) inhibitor, 7-nitroindazole (7-NI), for 10 consecutive days. The pharmacological interventions were performed by daily intraperitoneal (IP) injection at a dose of 80 mg/kg.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: 37212048
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Category | Signaling: nitric oxide pathway | Restored | Griess reagent assay | unreported | Tripathi MK et al. (2023) |
| Category | Signaling: nitric oxide pathway | Restored | Cyclic nucleotide activity assay | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synapse density | Ameliorated | Western blot | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: inhibitory neurons | Ameliorated | Immunofluorescence staining | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: excitatory neurons | Ameliorated | Immunofluorescence staining | unreported | Tripathi MK et al. (2023) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Restored | Golgi-Cox staining | unreported | Tripathi MK et al. (2023) |
| Neurophysiology | Oxidative stress in neurons | Ameliorated | Western blot | unreported | Tripathi MK et al. (2023) |
| Social behavior | Social memory | Restored | Three-chamber social approach test | unreported | Tripathi MK et al. (2023) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | unreported | Tripathi MK et al. (2023) |
| Emotion | Anxiety | Restored | Elevated plus maze test | unreported | Tripathi MK et al. (2023) |
| Learning & memory | Object recognition memory | Restored | Novel object recognition test | unreported | Tripathi MK et al. (2023) |
| Learning & memory | Object recognition memory | No adverse effect | Novel object recognition test | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Signaling: PKA pathway | No adverse effect | Cyclic nucleotide activity assay | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Signaling: nitric oxide pathway | Restored | Griess reagent assay | unreported | Tripathi MK et al. (2023) |
| Molecular profile | Signaling: nitric oxide pathway | Restored | Cyclic nucleotide activity assay | unreported | Tripathi MK et al. (2023) |
M_SHANK3_19_KO_HM_CDPPB-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
CDPPB ( SML0235, Sigma-Aldrich), dissolved in 0.5% methylcellulose, was administered at a dose of 10 mg/kg (i.p.) 30 min before behavioral testing in Shank3 exon 4-22 deleted homozygous mice (2 months age).
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neurophysiology | Synaptic plasticity: striatal ltd | Restored | Whole-cell patch clamp | 2-5 months | Wang X , et al. (2016) |
| Neurophysiology | Spontaneous post synaptic event frequency: excitatory currents | Refractory | Whole-cell patch clamp | NA | Wang X , et al. (2016) |
| Neurophysiology | Spontaneous post synaptic event amplitude: excitatory currents | No adverse effect | Whole-cell patch clamp | NA | Wang X , et al. (2016) |
| Neurophysiology | Presynaptic function | Refractory | High-frequency stimulation (hfs) | NA | Wang X , et al. (2016) |
| Neurophysiology | Action potential property: firing rate | Refractory | Whole-cell patch clamp | NA | Wang X , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Side effect | Grooming behavior assessments | 4-7 months | Wang X , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Social behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Molecular profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
M_SHANK3_19_KO_HM_CDPPB-2
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
CDPPB ( SML0235, Sigma-Aldrich), dissolved in 0.5% methylcellulose, was administered at a dose of 10 mg/kg (i.p.) for 7 days 30 min before behavioral testing,where mice were trained in a task every day, in Shank3 exon 4-22 deleted homozygous mice (2 months age).
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Social behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Learning & memory | Reward reinforced choice behavior | Ameliorated | Operant conditioning paradigm | Adult | Wang X , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Molecular profile | Protein localization: synapse | Ameliorated | Western blot | Adult | Wang X , et al. (2016) |
M_SHANK3_19_KO_HM_MPEP
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
MPEP (M5343, Sigma-Aldrich), dissolved in 0.9% normal saline, was administered at a dose of 20mg/kg (i.p.) 30 min before behavioral testing in Shank3 exon 4-22 deleted homozygous mice (2 months of age). MPEP is a known mGlur5 antagonist.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Motor phenotype | General locomotor activity | Restored | Open field test | Adult | Wang X , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Ameliorated | Grooming behavior assessments | 4-7 months | Wang X , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Social behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Molecular profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
M_SHANK3_19_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Shank3 exon 4-22 floxed mice were bred to CMV-cre mice to generate heterozygous Shank3 knockouts.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Motor phenotype | Grip strength | No change | Grip strength test | Adult | Wang X , et al. (2016) |
| Motor phenotype | General locomotor activity | No change | Open field test | Adult | Wang X , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Epsp-spike relationship | increased | Whole-cell patch clamp | P61 | Tzanoulinou S et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 8 weeks | Wang X , et al. (2016) |
| Neurophysiology | Neuronal activation | increased | Fluorescence microscopy | P60 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Action potential property: firing rate | increased | Whole-cell patch clamp | P61 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Action potential property: firing rate | no change | Whole-cell patch clamp | P67 | Tzanoulinou S et al. (2022) |
| Neurophysiology | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Repetitive behavior | Repetitive nose pokes | No change | Hole-board test | Adult | Wang X , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Sensory | Vision | No change | Morris water maze test | Adult | Wang X , et al. (2016) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Wang X , et al. (2016) |
| Social behavior | Social approach | no change | Three-chamber social approach test | P67 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | no change | Fiber photometry | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | decreased | Three-chamber social approach test | P61 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | decreased | Fiber photometry | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social scent marking or recognition | No change | Homing test | Adult | Wang X , et al. (2016) |
| Social behavior | Social memory | No change | Three-chamber social approach test | Adult | Wang X , et al. (2016) |
| Social behavior | Social interaction | No change | Reciprocal social interaction test | Adult | Wang X , et al. (2016) |
| Social behavior | Social approach | No change | Three-chamber social approach test | Adult | Wang X , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | P4 | Wang X , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Emotion | Anxiety | No change | Light-dark exploration test | Adult | Wang X , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 8 weeks | Wang X , et al. (2016) |
| Learning & memory | Spatial learning | No change | Morris water maze test | Adult | Wang X , et al. (2016) |
| Learning & memory | Olfactory learning and memory | No change | Olfactory habituation-dishabituation test | Adult | Wang X , et al. (2016) |
| Learning & memory | Cognitive flexibility | No change | Morris water maze test | Adult | Wang X , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Molecular profile | Gene expression | no change | Quantitative PCR (qRT-PCR) | P67 | Tzanoulinou S et al. (2022) |
| Molecular profile | Gene expression | increased | Quantitative PCR (qRT-PCR) | P61 | Tzanoulinou S et al. (2022) |
| Molecular profile | Protein localization: synapse | No change | Western blot | Adult | Wang X , et al. (2016) |
M_SHANK3_19_KO_TRPV4INHIB
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Pharmaceutical rescue of Shank3 het mice injected with LPS with ex-vivo bath application or injection of HC-067047 (Trpv4 inhibitor).
Allele Type: knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Motor phenotype | General locomotor activity | no adverse effect | Three-chamber social approach test | P61 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Action potential property: firing rate | restored | Whole-cell patch clamp | P61 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | ameliorated | Three-chamber social approach test | P61 | Tzanoulinou S et al. (2022) |
M_SHANK3_1_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified
Es cell line: Bruce4 C57BL/6
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Motor phenotype | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain morphology | No change | Histology | Unreported | Bozdagi O , et al. (2010) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain morphology | No change | Gross necroscopy | Unreported | Bozdagi O , et al. (2010) |
| Neurophysiology | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Repetitive behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Seizure | Seizures | No change | General observations | Unreported | Bozdagi O , et al. (2010) |
| Sensory | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Social behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Physiological parameters | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Communications | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Developmental profile | General characteristics | No change | General observations | Unreported | Bozdagi O , et al. (2010) |
| Developmental profile | Developmental trajectory | No change | General observations | P2-p14 | Bozdagi O , et al. (2010) |
| Emotion | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Immune response | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Learning & memory | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Maternal behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | Unreported | Bozdagi O , et al. (2010) |
| Molecular profile | Gene expression | Decreased | Quantitative pcr (qrt-pcr) | Unreported | Bozdagi O , et al. (2010) |
M_SHANK3_1_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified
Es cell line: Bruce4 C57BL/6
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Motor phenotype | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Motor phenotype | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Structural dendritic plasticity | Abnormal | Whole-cell patch clamp | Unreported | Bozdagi O , et al. (2010) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain morphology | No change | Histology | Unreported | Bozdagi O , et al. (2010) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain morphology | No change | Gross necroscopy | Unreported | Bozdagi O , et al. (2010) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Decreased | Whole-cell patch clamp | Unreported | Bozdagi O , et al. (2013) |
| Neurophysiology | Neuroreceptor activity | Decreased | Whole-cell patch clamp | Unreported | Bozdagi O , et al. (2013) |
| Neurophysiology | Synaptic transmission | Decreased | Field potential recordings | 3-4 weeks | Bozdagi O , et al. (2010) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Decreased | Field potential recordings | Unreported | Bozdagi O , et al. (2010) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | No change | Field potential recordings | Unreported | Bozdagi O , et al. (2010) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | Decreased | Whole-cell patch clamp | 3 months | Bozdagi O , et al. (2010) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Increased | Whole-cell patch clamp | NA | Bozdagi O , et al. (2010) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 3 months | Bozdagi O , et al. (2010) |
| Repetitive behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Repetitive behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Seizure | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Seizure | Seizures | No change | General observations | Unreported | Bozdagi O , et al. (2010) |
| Sensory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Sensory | Olfaction | No change | Olfactory habituation-dishabituation test | 2.5-4 months | Bozdagi O , et al. (2010) |
| Social behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Social behavior | Social interaction: opposite sex | Decreased | Reciprocal social interaction test | 2.5-4 months | Bozdagi O , et al. (2010) |
| Physiological parameters | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Physiological parameters | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Communications | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Decreased | Monitoring ultrasonic vocalizations | 2.5-4 months | Bozdagi O , et al. (2010) |
| Developmental profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Developmental profile | General characteristics | No change | General observations | Unreported | Bozdagi O , et al. (2010) |
| Developmental profile | Developmental trajectory | No change | General observations | P2-p14 | Bozdagi O , et al. (2010) |
| Emotion | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Emotion | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Immune response | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Immune response | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Learning & memory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Learning & memory | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Maternal behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Maternal behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2010) |
| Molecular profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | Unreported | Bozdagi O , et al. (2010) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 3 months | Bozdagi O , et al. (2010) |
| Molecular profile | Gene expression | Decreased | Quantitative pcr (qrt-pcr) | Unreported | Bozdagi O , et al. (2010) |
M_SHANK3_1_KO_HT_IGF1-H
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene followed by intraperitoneal injection at 240 microg/kg/day starting at PND 13-15 for 2 weeks of full-length IGF-1.
Allele Type: Partial knockout
Strain of Origin: Not specified
Genetic Background: Not Specified
Es cell line: Not specified
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Motor phenotype | Motor coordination and balance | Restored | Accelerating rotarod test | 4 weeks | Bozdagi O , et al. (2013) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Restored | Whole-cell patch clamp | 4 weeks | Bozdagi O , et al. (2013) |
| Neurophysiology | Neuroreceptor activity | Restored | Whole-cell patch clamp | 4 weeks | Bozdagi O , et al. (2013) |
| Repetitive behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Seizure | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Sensory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Social behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Physiological parameters | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Communications | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Developmental profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Emotion | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Immune response | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Learning & memory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Maternal behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Molecular profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
M_SHANK3_1_KO_HT_IGF1-L
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene followed by intraperitoneal injection at 120 microg/kg/day starting at PND 13-15 for 2 weeks of full-length IGF-1.
Allele Type: Partial knockout
Strain of Origin: Not specified
Genetic Background: Not Specified
Es cell line: Not specified
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Motor phenotype | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Ameliorated | Whole-cell patch clamp | 4 weeks | Bozdagi O , et al. (2013) |
| Repetitive behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Seizure | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Seizure | Absence seizures | Ameliorated | 16s rrna gene sequence analysis for identification of bacteria | Dfsd | Bozdagi O , et al. (2013) |
| Sensory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Social behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Physiological parameters | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Communications | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Developmental profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Emotion | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Immune response | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Learning & memory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Maternal behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Molecular profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
M_SHANK3_1_KO_HT_PIGF1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Targeted Cre/loxP mediated deletion of exon 4 to exon 9 of Shank3 gene followed by intraperitoneal injections at 10 microg/g/day for 2 weeks of active peptide derivative of IGF-1.
Allele Type: Partial knockout
Strain of Origin: Not specified
Genetic Background: Not Specified
Es cell line: Not specified
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Motor phenotype | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Restored | Whole-cell patch clamp | 4 weeks | Bozdagi O , et al. (2013) |
| Neurophysiology | Neuroreceptor activity | Restored | Whole-cell patch clamp | 4 weeks | Bozdagi O , et al. (2013) |
| Repetitive behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Seizure | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Sensory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Social behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Physiological parameters | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Communications | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Developmental profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Emotion | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Immune response | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Learning & memory | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Maternal behavior | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
| Molecular profile | NA | Not reported | NA | NA | Bozdagi O , et al. (2013) |
M_SHANK3_20_CKO_HM_NAC
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Conditional deletion of exons 4-22 of the Shank3 gene in 10-12 week old female mice by injecting AAV10- Cre virus, bilaterally, in the nucleus accumbens (NAc) to selectively knockdown Shank3 in the Nac
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Neurophysiology | Action potential property: firing rate | Decreased | In vivo local field potential (lfp) recordings | 3-4 months | Wang X , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Social behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2016) |
| Molecular profile | NA | Not reported | NA | NA | Wang X , et al. (2016) |
M_SHANK3_21_KI_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted knockin inversion of exons 13 to 16, that include the coding region of the PDZ domain, generated using the FLEx genetic switch strategy. The inverted insert is flanked by FRT and loxP sites. The knockin floxed mouse is called a functional knockout by authors.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | 2-3 months | Guo B , et al. (2019) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | Unreported | Mei Y , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | Unreported | Mei Y , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine morphology | Decreased | Immunofluorescence staining | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Immunofluorescence staining | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Increased | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Epsp-spike relationship | Decreased | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Presynaptic function: presynaptic fiber volley | No change | Field potential recordings | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Neurophysiology | Population spikes | Decreased | Field potential recordings | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 2-3 months | Guo B , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 2-3 months | Guo B , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Increased | General observations | Unreported | Mei Y , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Emotion | Exploratory activity | Decreased | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Increased | Zero maze test | Unreported | Mei Y , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Learning & memory | Object recognition memory | Decreased | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Molecular profile | Targeted expression | Decreased | Western blot | 2-3 months | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Western blot | Unreported | Mei Y , et al. (2016) |
M_SHANK3_21_KI_HM_REINSTATE-ADULT
Model Type: RESCUE-Genetic
Model Genotype: Homozygous
Mutation:
Conditional knock-in Shank3 mouse model in which exon 13 - 16 encoding the PDZ domain, were inverted with a Flex system were injected with AAV2/8-CaMKII-Cre-EGFP in the ACC to re-express most SHANK3 isoforms in the ACC but not the adjacent motor cortex.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Restored | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Ameliorated | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine morphology | Restored | Immunofluorescence staining | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Restored | Immunofluorescence staining | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Restored | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No adverse effect | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Ameliorated | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Restored | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Epsp-spike relationship | Restored | Whole-cell patch clamp | 2-3 months | Guo B , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Grooming behavior assessments | 2-3 months | Guo B , et al. (2019) |
| Repetitive behavior | Repetitive digging | Refractory | Marble-burying test | 2-3 months | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Ameliorated | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | Exploratory activity | Refractory | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Refractory | Elevated plus maze test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Refractory | Open field test | 2-3 months | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | Object recognition memory | Refractory | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | Targeted expression | Restored | Western blot | 2-3 months | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_22_KI_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with inverted exons 13-16 and the transgene CAGGS-CreER used as controls or parent models. Genotype of these mice is: Shank3fx/fx;CreER+/-.Mice received corn oil treatment (vehicle) at 2-4.5 months.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | Adult, p20-21 | Mei Y , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | Adult, p20-21 | Mei Y , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Immunohistochemistry | Adult, p20-21 | Mei Y , et al. (2016) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Neurophysiology | Presynaptic function: presynaptic fiber volley | No change | Field potential recordings | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Neurophysiology | Population spikes | Decreased | Field potential recordings | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | Adult, p20-21 | Mei Y , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | Adult, p20-21 | Mei Y , et al. (2016) |
| Social behavior | Rearing behavior | Decreased | Open field test | NA | Mei Y , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Developmental profile | Size/growth | Decreased | Body weight measurement | P20-21 | Mei Y , et al. (2016) |
| Emotion | Anxiety | Increased | Elevated zero maze test | Adult, p20-21 | Mei Y , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Molecular profile | Targeted expression | Decreased | Western blot | Adult, p20-21 | Mei Y , et al. (2016) |
| Molecular profile | Protein expression level evidence | No change | Western blot | Adult, p20-21 | Mei Y , et al. (2016) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | Adult, p20-21 | Mei Y , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | Adult, p20-21 | Mei Y , et al. (2016) |
M_SHANK3_22_KI_HM_REINSTATE-ADULT
Model Type: RESCUE-Genetic
Model Genotype: Homozygous
Mutation:
Mice with the inverted exons 13-16 re-inverted using tamoxifen-inducible globally expressed CAGGS-CreER. The pCAGGS promoter includes the CMV early enhancer with chicken beta-actin promoter. Adult rescue was performed by tamoxifen treament by oral gavaging at 2-4.5 months while control group received corn oil. Genotype of mice: Shank3fx/fx;CreER+/-.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Motor phenotype | Motor coordination and balance | Refractory | Accelerating rotarod test | Adult | Mei Y , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | Adult | Mei Y , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No adverse effect | Whole-cell patch clamp | 3-7 months, p20-21 | Mei Y , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Restored | Immunohistochemistry | Adult | Mei Y , et al. (2016) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No adverse effect | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Presynaptic function: presynaptic fiber volley | No adverse effect | Field potential recordings | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No adverse effect | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Population spikes | Restored | Field potential recordings | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Restored | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No adverse effect | Whole-cell patch clamp | 3-7 months | Mei Y , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Restored | Grooming behavior assessments | Adult | Mei Y , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Social behavior | Social approach | Ameliorated | Three-chamber social approach test | Adult | Mei Y , et al. (2016) |
| Social behavior | Rearing behavior | Refractory | Open field test | Adult | Mei Y , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Emotion | Anxiety | Refractory | Elevated zero maze test | Adult | Mei Y , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Molecular profile | Targeted expression | Ameliorated | Immunohistochemistry | Adult | Mei Y , et al. (2016) |
| Molecular profile | Protein expression level evidence | Restored | Western blot | Adult | Mei Y , et al. (2016) |
M_SHANK3_22_KI_HM_REINSTATE-JUVENILE
Model Type: RESCUE-Genetic
Model Genotype: Homozygous
Mutation:
Mice with the inverted exons 13-16 re-inverted using tamoxifen-inducible globally expressed CAGGS-CreER. The pCAGGS promoter includes the CMV early enhancer with chicken beta-actin promoter. Juvenile rescue was performed by tamoxifen treament by oral gavaging at P20 to 21, while control group received corn oil. Genotype of mice: Shank3fx/fx;CreER+/-.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Motor phenotype | Motor coordination and balance | Restored | Accelerating rotarod test | Adult | Mei Y , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | Adult | Mei Y , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Social behavior | Rearing behavior | Refractory | Open field test | Adult | Mei Y , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Developmental profile | Size/growth | Side effect | Body weight measurement | P20-21 | Mei Y , et al. (2016) |
| Emotion | Anxiety | Restored | Elevated zero maze test | Adult | Mei Y , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Mei Y , et al. (2016) |
| Molecular profile | Targeted expression | Ameliorated | Western blot | Adult | Mei Y , et al. (2016) |
M_SHANK3_23_KI_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
KI of neo-STOP cassette inserted in intron 12, between exon 12 and exon 13, resulting in loss of higher mol weight Shank3 isoforms, bred to homozygosity. All Shank3 protein expressed in these mutants lacks the PDX domain.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57BL/6J
Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Motor phenotype | General locomotor activity | No change | Open field test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Neurophysiology | Synaptic transmission | No change | Field potential recordings | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Decreased | Whole-cell patch clamp | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | No change | Whole-cell patch clamp | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Neurophysiology | Presynaptic function: paired-pulse depression (ppd) | No change | Paired-pulse ratio | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Neurophysiology | Epsp-spike relationship | No change | Field potential recordings | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social memory: long-term social memory | Decreased | Social recognition test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social interaction | Decreased | Partition test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social approach | No change | Three-chamber social approach test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Emotion | Thigmotaxis | Increased | Morris water maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Exploratory activity | Decreased | Home cage behavior | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Anxiety | No change | Open field test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Anxiety | No change | Elevated plus maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Anxiety | No change | Light-dark exploration test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Learning & memory | Spatial reference memory | Decreased | Morris water maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Learning & memory | Spatial learning | Decreased | Morris water maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Molecular profile | Targeted expression | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
M_SHANK3_23_KI_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Neo-STOP cassette was inserted in intron 12, between exon 12 and exon 13, of the Shank3 gene and results in the loss of two higher molecular weight isoforms of Shank3, and results in a protein lacking the PDZ domain.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57BL/6J
Es cell line: 129s6SvEvTac
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Motor phenotype | Motor coordination and balance | Increased | Accelerating rotarod test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Motor phenotype | General locomotor activity | No change | Open field test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Decreased | Whole-cell patch clamp | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | 4-6 weeks | Jaramillo TC , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Social behavior | Social memory: long-term social memory | Decreased | Social recognition test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social interaction | No change | Partition test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social interaction | No change | Reciprocal social interaction test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Emotion | Thigmotaxis | No change | Morris water maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Exploratory activity | No change | Home cage behavior | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Exploratory activity | Decreased | Home cage behavior | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Emotion | Anxiety | No change | Light-dark exploration test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Learning & memory | Spatial learning | No change | Morris water maze test | 5.4- 9 weeks | Jaramillo TC , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Jaramillo TC , et al. (2016) |
| Molecular profile | Targeted expression | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 4 weeks | Jaramillo TC , et al. (2016) |
M_SHANK3_24_KD
Model Type: Genetic
Model Genotype: Wildtype
Mutation:
Seven-week-old wildtype C57BL/6 male mice were injected with Shank3-siRNA (1424750, NM_021423.2), bilaterally in the dorsal striatum, using a stereotaxic apparatus, resulting in a reduction of Shank3 transcript levels to 0.4 fold compared with control siRNA injected mice.
Allele Type: Knockdown
Strain of Origin: C57BL/6
Genetic Background: C57BL/6
Es cell line: NA
Model Source: Daehan BioLink (Eumsung, Chungbuk, Republic of Korea)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | Adult | Lee Y , et al. (2019) |
| Repetitive behavior | Repetitive digging | No change | Home cage behavior | Adult | Lee Y , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Social behavior | Social memory | No change | U-field assay | Adult | Lee Y , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Learning & memory | Object recognition memory | Decreased | Object preference test | Adult | Lee Y , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Lee Y , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Quantitative pcr (qrt-pcr) | Adult | Lee Y , et al. (2019) |
M_SHANK3_24_KI_HM_S685I
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with autism-linked human missense mutation S685I knocked in using CRISPR-CAS9. A FLAG-tagged Shank3 construct is used.
Allele Type: ASD LOF mutation
Strain of Origin: FVB/N
Genetic Background: FVB
Es cell line: FVB/N
Model Source: PMID 30610205
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | Adult | Wang L , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | Adult | Wang L , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size | No change | Western blot | Adult | Wang L , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | No change | Immunostaining | Adult | Wang L , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Immunostaining | Adult | Wang L , et al. (2019) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | Adult | Wang L , et al. (2019) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Vertical jumping or back flipping | No change | Open field test | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Allogrooming: perseveration | Increased | Resident-intruder test | Adult | Wang L , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | Adult | Wang L , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Wang L , et al. (2019) |
| Social behavior | Social interaction: opposite sex | No change | Resident-intruder test | Adult | Wang L , et al. (2019) |
| Social behavior | Social dominance | Increased | Tube test of social dominance | Adult | Wang L , et al. (2019) |
| Social behavior | Social approach | No change | Three-chamber social approach test | Adult | Wang L , et al. (2019) |
| Social behavior | Aggression | No change | Resident-intruder test | Adult | Wang L , et al. (2019) |
| Physiological parameters | Reproductive function | No change | General observations | Adult | Wang L , et al. (2019) |
| Communications | Ultrasonic vocalization: isolation induced | Abnormal | Monitoring ultrasonic vocalizations | P2-p16 | Wang L , et al. (2019) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | No change | Monitoring ultrasonic vocalizations | Adult | Wang L , et al. (2019) |
| Developmental profile | Size/growth | No change | Body weight measurement | 0-12 weeks | Wang L , et al. (2019) |
| Developmental profile | Mortality/lethality | No change | General observations | P0 | Wang L , et al. (2019) |
| Developmental profile | Developmental trajectory | No change | General observations | P13-p14 | Wang L , et al. (2019) |
| Emotion | Anxiety | No change | Open field test | Adult | Wang L , et al. (2019) |
| Emotion | Anxiety | No change | Elevated plus maze test | Adult | Wang L , et al. (2019) |
| Molecular profile | Targeted expression | No change | Western blot | Adult | Wang L , et al. (2019) |
| Molecular profile | Protein expression level evidence | No change | Western blot | Adult | Wang L , et al. (2019) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | Adult | Wang L , et al. (2019) |
| Molecular profile | Protein binding | No change | Immunoprecipitation | Adult | Wang L , et al. (2019) |
| Molecular profile | Protein binding | Decreased | Immunoprecipitation | Adult | Wang L , et al. (2019) |
M_SHANK3_25_KI_HT_S685I
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Heterozygous mice with autism-linked human missennse mutation S685I knocked in using CRISPR-CAS9. FLAG-tagged Shank3 construct is used.
Allele Type: ASD LOF mutation
Strain of Origin: FVB/N
Genetic Background: FVB
Es cell line: FVB/N
Model Source: PMID 30610205
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | Adult | Wang L , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Vertical jumping or back flipping | No change | Open field test | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | Adult | Wang L , et al. (2019) |
| Repetitive behavior | Allogrooming: perseveration | No change | Resident-intruder test | Adult | Wang L , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | Adult | Wang L , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Wang L , et al. (2019) |
| Social behavior | Social dominance | No change | Tube test of social dominance | Adult | Wang L , et al. (2019) |
| Social behavior | Social dominance | Increased | Tube test of social dominance | Adult | Wang L , et al. (2019) |
| Social behavior | Social approach | No change | Three-chamber social approach test | Adult | Wang L , et al. (2019) |
| Physiological parameters | Reproductive function | No change | General observations | Adult | Wang L , et al. (2019) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | P2-p16 | Wang L , et al. (2019) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | No change | Monitoring ultrasonic vocalizations | Adult | Wang L , et al. (2019) |
| Developmental profile | Size/growth | No change | Body weight measurement | 0-12 weeks | Wang L , et al. (2019) |
| Developmental profile | Mortality/lethality | No change | General observations | P0 | Wang L , et al. (2019) |
| Emotion | Anxiety | No change | Open field test | Adult | Wang L , et al. (2019) |
| Emotion | Anxiety | No change | Elevated plus maze test | Adult | Wang L , et al. (2019) |
| Molecular profile | Protein binding | Decreased | Immunoprecipitation | Adult | Wang L , et al. (2019) |
M_SHANK3_25_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Model with exons 13 to 16 (containing the PDZ domain) of the Shank3 gene replaced with a NEO cassette. This model is identical to the construct of M_SHANK3_4_KO_HM but on a different genetic background; M_SHANK3_4_KO_HM
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | () |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | () |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size | Decreased | Immunofluorescence staining | 2-6 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 2-6 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 2-6 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | 2-6 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine morphology | Decreased | Fluorescence microscopy | 2-6 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Fluorescence microscopy | 2-6 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | Decreased | Sholl analysis | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Neuronal activation | Increased | Immunohistochemistry | Adult, 2-5 months, 9-12 months | () |
| Neurophysiology | Synaptic transmission: excitatory | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Synaptic plasticity: anterior cingulate cortex ltp | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Increased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Neuronal activation: non-familiar social interaction | Decreased | Fiber photometry | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Membrane potential | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Intrinsic membrane properties | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Epsp-spike relationship | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Action potential property: threshold | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Action potential property: firing rate | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Action potential property: amplitude | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Action potential properties: half-width | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | () |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 2-3 months | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | () |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | () |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | Adult | () |
| Social behavior | Social interaction: with juveniles | Decreased | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | () |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | () |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | () |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | () |
| Emotion | Exploratory activity | Decreased | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 2-3 months | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | () |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | () |
| Learning & memory | Object recognition memory | Decreased | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | () |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | () |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_ACC-ACT-1
Model Type: RESCUE-Procedural
Model Genotype: Homozygous
Mutation:
Shank3 null mice with knockout of the PDZ domain were injected AAV2/8-CaMKIIa-hChR2-EYFP to deliver channelrhodopsin-2 (ChR2) to pyramidal neurons in the ACC to selectively enhance the activity of ACC pyramidal neurons transiently. Whole-cell recordings show that ACC pyramidal neurons exhibit activation in response to 10 Hz of optogenetic stimulation with a 473-nm laser. Control Shank3 null mice were injected EYFP.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neurophysiology | Neuronal activation | Restored | Immunohistochemistry | 2-3 months | Guo B , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Restored | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | Anxiety | Restored | Open field test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | Restored | Elevated plus maze test | 2-3 months | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_ACC-ACT-2
Model Type: RESCUE-Procedural
Model Genotype: Homozygous
Mutation:
Shank3 KO mice were injected with a mixture of AAV2/8-CaMKIIa-Cre-EGFP and AAV2/8-EF1a-DIO-hM3D(Gq)-mCherry into the ACC and expression of hM3Dq designer receptors was verified in ACC pyramidal neurons that are exclusively activated by designer drugs (DREADDs) to enhance ACC pyramidal cell activity over extended periods. 0.7 mg per kg clozapine N-oxide (CNO) was administered before the test to activate ACC pyramidal neurons.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Grooming behavior assessments | 2-3 months | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Restored | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_CX546-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Shank3 KO mice were subcutaneously injected with CX546 at 5mg per kg in 1% DMSO for 7 days.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Refractory | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_CX546-2
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Shank3 KO mice were subcutaneously injected with CX546 at 10mg per kg in 1% DMSO for 7 days.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Ameliorated | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_CX546-3
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Shank3 KO mice were subcutaneously injected with CX546 at 20 mg per kg in 1% DMSO for 7 days.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Restored | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_CX546-4
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
400 micromolar CX546 was microinjected into the anterior cingulate cortex of the shank3 KO mice.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Ameliorated | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Restored | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_25_KO_HM_LPS
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous; hemizygous
Mutation:
Shank3 mutant mice were treated with lipopolysaccharide (50microgram/kg, i.p., acute)
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: The Jackson Laboratory
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
| Motor phenotype | NA | Not reported | NA | NA | () |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | () |
| Neurophysiology | Neuronal activation | Refractory | Immunohistochemistry | Adult | () |
| Repetitive behavior | NA | Not reported | NA | NA | () |
| Seizure | NA | Not reported | NA | NA | () |
| Sensory | NA | Not reported | NA | NA | () |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | Adult | () |
| Physiological parameters | NA | Not reported | NA | NA | () |
| Communications | NA | Not reported | NA | NA | () |
| Developmental profile | NA | Not reported | NA | NA | () |
| Emotion | NA | Not reported | NA | NA | () |
| Immune response | Cytokine levels: pro-inflammatory | Side effect | Elisa | Adult, 2-5 months, 9-12 months | () |
| Learning & memory | NA | Not reported | NA | NA | () |
| Maternal behavior | NA | Not reported | NA | NA | () |
| Molecular profile | NA | Not reported | NA | NA | () |
M_SHANK3_25_KO_HM_S1DZ-INACT
Model Type: RESCUE-Procedural
Model Genotype: Homozygous
Mutation:
Neural activity was optogenetically suppressed in the primary somatosensory cortex dysgranular zone in Shank3 mutant mice.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: The Jackson Laboratory
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | () |
| Motor phenotype | NA | Not reported | NA | NA | () |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | () |
| Neurophysiology | NA | Not reported | NA | NA | () |
| Repetitive behavior | NA | Not reported | NA | NA | () |
| Seizure | NA | Not reported | NA | NA | () |
| Sensory | NA | Not reported | NA | NA | () |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | Adult | () |
| Physiological parameters | NA | Not reported | NA | NA | () |
| Communications | NA | Not reported | NA | NA | () |
| Developmental profile | NA | Not reported | NA | NA | () |
| Emotion | NA | Not reported | NA | NA | () |
| Immune response | NA | Not reported | NA | NA | () |
| Learning & memory | NA | Not reported | NA | NA | () |
| Maternal behavior | NA | Not reported | NA | NA | () |
| Molecular profile | NA | Not reported | NA | NA | () |
M_SHANK3_26_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with selective knockout of Shank3 in the anterior cingulate cortex were generated CRISPR/Cas9 mediated genome editing by administering an AAV2/9 vector harboring Staphylococcus aureus Cas9 (SaCas9) and a single-guide RNA (sgRNA) to adult WT mice. This eliminated Shank3 alpha, beta and gamma isoforms in the anterior cingulate cortex.
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Increased | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | 2-3 months | Guo B , et al. (2019) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Increased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Neurophysiology | Epsp-spike relationship | Decreased | Whole-cell patch clamp | 2-6 months | Guo B , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 2-3 months | Guo B , et al. (2019) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | 2-3 months | Guo B , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 2-3 months | Guo B , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 2-3 months | Guo B , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | No change | Elevated plus maze test | 2-3 months | Guo B , et al. (2019) |
| Emotion | Anxiety | No change | Open field test | 2-3 months | Guo B , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Learning & memory | Object recognition memory | No change | Novel object recognition test | 2-3 months | Guo B , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Guo B , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Western blot | 2-3 months | Guo B , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Guo B , et al. (2019) |
M_SHANK3_27_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mice with selective ablation of Shank3 below cervical level 2 were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 with mice expressing Cre recombinase below cervical level 2 (Cdx2-Cre; Shank3f/+)
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: PMID 31398341; Akyol et al., 2008; Eric Fearon; RRID:IMSR_JAX:009350
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | No change | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_28_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mice with selective ablation of Shank3 in all DRG, trigeminal, and sympathetic ganglia somatosensory neurons were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 with mice expressing Advillin-Cre recombinase (Advillin-Cre; Shank3f/+).
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: PMID 31398341; Hasegawa et al, 2007; Fan Wang; RRID:IMSR_JAX:032536
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Decreased | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the spinal cord: sensory and motor tracts | Decreased | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event frequency: inhibitory currents | Increased | Whole-cell patch clamp | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event frequency: excitatory currents | No change | Whole-cell patch clamp | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event amplitude: inhibitory currents | Decreased | Whole-cell patch clamp | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event amplitude: excitatory currents | Decreased | Whole-cell patch clamp | Adult | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_29_CKO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with selective ablation of Shank3 in all DRG, trigeminal, and sympathetic ganglia somatosensory neurons were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 with mice expressing Advillin-Cre recombinase (Advillin-Cre; Shank3f/f).
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: PMID 31398341; Hasegawa et al, 2007; Fan Wang; RRID:IMSR_JAX:032536
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the spinal cord: sensory and motor tracts | Decreased | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | No change | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Immunohistochemistry | 6-8 weeks | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_30_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain, the positive selection marker neo cassette is retained; M_SHANK3_4_KO_HT
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the spinal cord: sensory and motor tracts | Decreased | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | Synaptic transmission | Decreased | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Intrinsic membrane properties | Increased | Whole-cell current clamp | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | Action potential property: firing rate | Decreased | Whole-cell voltage clamp | Adult | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensory-evoked response: excitation: tactile stimulus | No change | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | Size/growth | Decreased | Body weight measurement | 3 weeks | Orefice LL , et al. (2019) |
| Developmental profile | Mortality/lethality | No change | Survival analysis | 6 weeks | Orefice LL , et al. (2019) |
| Developmental profile | General characteristics | Decreased | Macroscopic analysis | 3 weeks | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | Decreased | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | Decreased | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_30_KO_HT_ISOGUVACINE-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Mice with targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain are treated acutely with Isoguvacine, 2 mg/kg, i.p..
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No adverse effect | Acoustic startle reflex test | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_30_KO_HT_ISOGUVACINE-2
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Mice with targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain are treated acutely with Isoguvacine, 10micromolar (=2mg/kg) s.c.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Sensory-evoked response: excitation: tactile stimulus | Side effect | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_30_KO_HT_ISOGUVACINE-3
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Mice with targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain are treated with chronic isoguvacine at 2mg/kg, i.p. from P1 to P42.
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Restored | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No adverse effect | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Restored | Immunohistochemistry | 6 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Refractory | Immunohistochemistry | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Synaptic transmission | Refractory | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Synaptic transmission | Restored | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event frequency: inhibitory currents | Restored | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event frequency: excitatory currents | Restored | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event amplitude: inhibitory currents | Restored | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | Spontaneous post synaptic event amplitude: excitatory currents | Restored | Whole-cell patch clamp | 6 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Grooming behavior assessments | 6 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Restored | Marble-burying test | 6 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Refractory | Textured novel object recognition test (t-nort) | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No adverse effect | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | Refractory | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Restored | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | Size/growth | Restored | Body weight measurement | 3 weeks | Orefice LL , et al. (2019) |
| Developmental profile | Mortality/lethality | No adverse effect | Survival analysis | 6 weeks | Orefice LL , et al. (2019) |
| Developmental profile | General characteristics | Restored | Macroscopic analysis | 3 weeks | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Refractory | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No adverse effect | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Restored | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Restored | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | Refractory | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_31_KI_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase; M_SHANK3_21_KI_HM.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Decreased | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | Decreased | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_31_KI_HT-REINSTATEMENT-ADVILLIN
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase. In the presence of Cre, the Shank3 PDZ domain is aligned into the proper orientation resulting in restored expression of most major SHANK3 isoforms. Parents mice were crossed with Advillin-Cre to restore Shank3 expression in all DRG, trigeminal, and sympathetic ganglia neurons.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No adverse effect | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No adverse effect | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Restored | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Refractory | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Ameliorated | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Restored | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No adverse effect | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No adverse effect | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Ameliorated | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Ameliorated | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No adverse effect | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Restored | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Restored | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | Refractory | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_31_KI_HT-REINSTATEMENT-CDX2
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase. In the presence of Cre, the Shank3 PDZ domain is aligned into the proper orientation resulting in restored expression of most major SHANK3 isoforms. Parents mice were crossed with Cdx2-Cre to restore Shank3 expression in cells below cervical level 2.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No adverse effect | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No adverse effect | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Ameliorated | Marble-burying test | 6-8 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Restored | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No adverse effect | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Response to air puff | 6-8 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No adverse effect | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Ameliorated | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 6-8 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Ameliorated | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No adverse effect | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Restored | Elevated plus maze test | 6-8 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Restored | Open field test | 6-8 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | Refractory | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_31_KI_HT_REINSTATEMENT-ADVILLIN-ICRE-1
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^FX/+) in the postnatal period (P28-P33) were generated by crossing mice possessing cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase, and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No adverse effect | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No adverse effect | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Refractory | Marble-burying test | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Refractory | Marble-burying test | 6 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Refractory | Textured novel object recognition test (t-nort) | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No adverse effect | Acoustic startle reflex test | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Restored | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No adverse effect | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Refractory | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Refractory | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No adverse effect | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Refractory | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Refractory | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | Refractory | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_32_KI_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase, Shank3^FX/FX; M_SHANK3_21_KI_HM.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the spinal cord: sensory and motor tracts | Decreased | Immunohistochemistry | 6-8 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Immunohistochemistry | 6-8 weeks | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_32_KI_HM-REINSTATEMENT-ADVILLIN
Model Type: RESCUE-Genetic
Model Genotype: Homozygous
Mutation:
Mice with cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) that functions as a knockout allele in the absence of Cre recombinase. In the presence of Cre, the Shank3 PDZ domain is aligned into the proper orientation resulting in restored expression of most major SHANK3 isoforms. Parents mice were crossed with Advillin-Cre to restore Shank3 expression in all DRG, trigeminal, and sympathetic ganglia neurons; Advillin-Cre; Shank3^FX/FX.
Allele Type: LOF knockin
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratories; Guoping Feng; RRID:IMSR_JAX:028800; Mei et al., 2016
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the spinal cord: sensory and motor tracts | Restored | Immunohistochemistry | 6-8 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Restored | Immunohistochemistry | 6-8 weeks | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Restored | Immunohistochemistry | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_33_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain, the positive selection marker neo cassette is retained; M_SHANK3_4_KO_HT
Allele Type: Knockout
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: Jackson Laboratories; RRID:IMSR_JAX:017688
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: gaba-r: gabaa | No change | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the spinal cord: sensory and motor tracts | Decreased | Immunohistochemistry | Adult | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Western blot | 6-8 weeks | Orefice LL , et al. (2019) |
| Molecular profile | Targeted expression | Decreased | Immunohistochemistry | 6-8 weeks | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_34_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^f/+) in the early postnatal period (P5) were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratory; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Abnormal | Immunohistochemistry | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 6 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Increased | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_35_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^f/+) in the early postnatal period (P10) were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratory; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Increased | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Abnormal | Immunohistochemistry | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | Increased | Marble-burying test | 6 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Decreased | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | Decreased | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_36_CKO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Mutant mice with sensory neuron specific inducible deletion of Shank3 (Advillin-CreERT2; Shank3^f/+) in the early postnatal period (P28) were generated by crossing Shank3f mice with loxp sites flanking exons 13 and 16 and transgenic Advillin-CreERT2 driver mice that expresse a tamoxifen-inducible iCre recombinase directed by endogenous Avil (advillin) promoter elements in the dorsal root ganglia and sensory neurons.
Allele Type: Conditional loss-of-function
Strain of Origin: -
Genetic Background: C57BL/6J*129/SvEv*CD1
Es cell line: -
Model Source: Jackson Laboratory; John Wood; RRID:IMSR_JAX:032027 (Lau et al, 2011)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Motor phenotype | General locomotor activity | No change | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: interneurons | Abnormal | Immunohistochemistry | 6 weeks | Orefice LL , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | 6 weeks | Orefice LL , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Response to air puff | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating: tactile cue | Increased | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6 weeks | Orefice LL , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Emotion | Habituation to aversive stimuli | Decreased | Prepulse inhibition | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Exploratory activity | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | No change | Elevated plus maze test | 6 weeks | Orefice LL , et al. (2019) |
| Emotion | Anxiety | No change | Open field test | 6 weeks | Orefice LL , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Learning & memory | Object recognition memory: long-term recall | No change | Novel object recognition test | 6 weeks | Orefice LL , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2019) |
M_SHANK3_37_CKO_HU-NPC-CX
Model Type: Genetic LOF
Model Genotype: Chimeric
Mutation:
Human neuronal-chimeric mouse generated by transplanting gfp labelled control cells and human npc labelled with mcherry from an asd patient with a heterozygous truncated shank3 mutation into the motorcortex or the lateral ventricles of immune-deficient newborn mice at p1-2. transplanted mice were tested at at 30 days post-transplantation. the asd patient carried a de novo frameshift mutation which leads to a premature stop codon.
Allele Type: Wildtype
Strain of Origin: NA
Genetic Background: NOD/SCID
Es cell line: NA
Model Source: Charles Rivers
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal size | Decreased | Immunohistochemistry | Adult | Vitrac A et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal morphology | No change | Immunohistochemistry | Adult | Vitrac A et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine morphology | No change | Immunohistochemistry | Adult | Vitrac A et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Anatomical projections and connectivity | Decreased | Immunohistochemistry | Adult | Vitrac A et al. (2020) |
| Neurophysiology | Neuronal apoptosis | No change | Immunohistochemistry | Adult | Vitrac A et al. (2020) |
M_SHANK3_37_KD
Model Type: Genetic
Model Genotype: Wildtype
Mutation:
AAV-shShank3-luczsGreen were injected into nucleus accumbens of WT mice, D1R:Cre (MMRRC Stock No: 37156-JAX), and Drd1a-tdTomato reporter mice during early postnatal development (P6).
Allele Type: knockdown
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Neurophysiology | Membrane potential | increased | Whole-cell patch clamp | P60 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Intrinsic membrane properties | no change | Whole-cell patch clamp | P60 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Action potential property: firing rate | abnormal | Whole-cell patch clamp | P60 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Action potential property: firing rate | increased | Whole-cell patch clamp | P60 | Tzanoulinou S et al. (2022) |
| Neurophysiology | Action potential property: after hyperpolarization | no change | Whole-cell patch clamp | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social memory | no change | Three-chamber social approach test | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social interaction: with juveniles | decreased | Three-chamber social approach test | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | decreased | Three-chamber social approach test | P60 | Tzanoulinou S et al. (2022) |
| Emotion | Anxiety | no change | Elevated zero maze test | P60 | Tzanoulinou S et al. (2022) |
| Molecular profile | Targeted expression | no change | Western blot | P60 | Tzanoulinou S et al. (2022) |
| Molecular profile | Targeted expression | decreased | Western blot | P60 | Tzanoulinou S et al. (2022) |
| Molecular profile | Gene expression | increased | Quantitative PCR (qRT-PCR) | P30 | Tzanoulinou S et al. (2022) |
| Molecular profile | Gene expression | no change | Quantitative PCR (qRT-PCR) | P30 | Tzanoulinou S et al. (2022) |
| Molecular profile | Differential gene expression | abnormal | RNA sequencing | P30 | Tzanoulinou S et al. (2022) |
M_SHANK3_37_KD_CNO
Model Type: RESCUE-Genetic
Model Genotype: Wildtype
Mutation:
Chemogenetic rescue of D1R:Cre (MMRRC Stock No: 37156-JAX) reporter mice injected with AAV-shShank3-luczsGreen during early postnatal development (P6). AAV-DIO-hM4DimCherry (inhibitroy DREADD) injected after P30 then behavioral assay was done 30 mins after CNO injection.
Allele Type: knockdown
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Motor phenotype | General locomotor activity | side effect | Three-chamber social approach test | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | ameliorated | Three-chamber social approach test | P60 | Tzanoulinou S et al. (2022) |
M_SHANK3_37_KD_TRPV4INHIB
Model Type: RESCUE-Pharmaceutical
Model Genotype: Wildtype
Mutation:
Pharmaceutical rescue of D1R:Cre (MMRRC Stock No: 37156-JAX) reporter mice injected with AAV-shShank3-luczsGreen during early postnatal development (P6) with ex-vivo bath application or injection of HC-067047 (Trpv4 inhibitor).
Allele Type: knockdown
Strain of Origin: -
Genetic Background: C57BL/6J
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Neurophysiology | Action potential property: firing rate | restored | Whole-cell patch clamp | P60 | Tzanoulinou S et al. (2022) |
| Social behavior | Social approach | restored | Three-chamber social approach test | P57-74 | Tzanoulinou S et al. (2022) |
M_SHANK3_3_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Deletion of exon 21 of Shank3 resulting in lack of entire C-termain portion of Shank3 (the article reporting this model was retracted, however phenotypic data for the model has been retained following the retraction notification and MGI).
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: Not Specified
Es cell line: Not Specified
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Motor phenotype | Motor coordination and balance | No change | Balance beam test | P50-60 | Matas E et al. (2021) |
| Motor phenotype | Gait | No change | Gait | P50-60 | Matas E et al. (2021) |
| Motor phenotype | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: AMPA receptors | No change | Quantitative PCR (qRT-PCR) | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: AMPA receptors | No change | Western blot | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: AMPA receptors | Decreased | Western blot | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: Purkinje cells | No change | Immunostaining | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 2 months | Bangash MA , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | 2 months | Bangash MA , et al. (2011) |
| Neurophysiology | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission | Decreased | Whole-cell patch clamp | Unreported | Bangash MA , et al. (2011) |
| Repetitive behavior | Self grooming | No change | Grooming behavior assessments | P50-60 | Matas E et al. (2021) |
| Repetitive behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Repetitive behavior | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | P50-60 | Matas E et al. (2021) |
| Social behavior | Social approach | No change | Three-chamber social approach test | P50-60 | Matas E et al. (2021) |
| Social behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Social behavior | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | Size/growth | No change | General observations | Unreported | Bangash MA , et al. (2011) |
| Developmental profile | General characteristics | No change | General observations | Unreported | Bangash MA , et al. (2011) |
| Emotion | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Bangash MA , et al. (2011) |
| Molecular profile | Signaling | Decreased | Two-dimensional flourescence difference gel electrophoresis (2-dige) | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Protein ubiquitination | Increased | Western blot | Unreported | Bangash MA , et al. (2011) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 8 weeks | Bangash MA , et al. (2011) |
M_SHANK3_4_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain, the positive selection marker neo cassette is retained.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: R1
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Motor phenotype | Swimming ability | Decreased | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | 2-3 months, 6-7 months | Rhine MA , et al. (2019) |
| Motor phenotype | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Motor phenotype | General locomotor activity | Decreased | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Home cage behavior | 9-10 weeks | Fourie C , et al. (2018) |
| Motor phenotype | General locomotor activity | Decreased | Home cage behavior | 9-10 weeks | Fourie C , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Motor phenotype | Righting response | No change | Righting reflex test | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Paw preference | Decreased | General observations | P30, p60 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Negative geotaxis | No change | Negative geotaxis test | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Motor phenotype | General locomotor activity | Decreased | Marble-burying test | Adult | Kabitzke PA , et al. (2017) |
| Motor phenotype | General locomotor activity | Decreased | General observations | NA | Kabitzke PA , et al. (2017) |
| Motor phenotype | Gait | No change | General observations | P30, p60 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 5-6 weeks | Pea J , et al. (2011) |
| Motor phenotype | General locomotor activity | No change | Open field test | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Event related oscillations (eros) in electroencephalography (eeg) | no change | In vivo local field potential (LFP) recordings | 3-4 months | Tao K et al. (2022) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: oxytocin expressing | Decreased | Immunofluorescence staining | 7-10 weeks | Sgritta M , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | No change | Immunofluorescence staining | 7-10 weeks | Sgritta M , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: gaba-r: gabaa | Decreased | Quantitative pcr (qrt-pcr) | NA | Tabouy L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Post-synaptic density size | Decreased | Electron microscopy | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Increased | Sholl analysis | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Histology | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | No change | Magnetic resonance imaging (mri) | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Increased | Magnetic resonance imaging (mri) | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain cytoarchitecture | No change | Histology | 5-6 weeks | Pea J , et al. (2011) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain anatomy | No change | Histology | 5-6 weeks | Pea J , et al. (2011) |
| Neurophysiology | Neuronal activation: non-familiar social interaction | increased | Social recognition test | 3-4 months | Tao K et al. (2022) |
| Neurophysiology | Neuronal activation: familiar social interaction | decreased | Social recognition test | 3-4 months | Tao K et al. (2022) |
| Neurophysiology | Action potential property: threshold | increased | In vivo local field potential (LFP) recordings | 3-4 months | Tao K et al. (2022) |
| Neurophysiology | Action potential property: firing rate | no change | Social recognition test | 3-4 months | Tao K et al. (2022) |
| Neurophysiology | Action potential property: firing pattern | no change | In vivo local field potential (LFP) recordings | 3-4 months | Tao K et al. (2022) |
| Neurophysiology | Action potential property: amplitude | decreased | In vivo local field potential (LFP) recordings | 3-4 months | Tao K et al. (2022) |
| Neurophysiology | Ocular dominance plasticity | Decreased | Measurement of ocular dominance index | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | No change | Whole-cell patch clamp | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | Membrane potential | No change | Whole-cell patch clamp | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | Intrinsic membrane properties | No change | Whole-cell patch clamp | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | Adaptation of action potential firing | Decreased | Whole-cell patch clamp | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | Action potential property: firing rate | No change | Whole-cell patch clamp | P25-p32 | Tatavarty V et al. (2020) |
| Neurophysiology | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Neurophysiology | Synaptic plasticity: ventral tegmental area ltp | Decreased | Whole-cell patch clamp | 7-10 weeks | Sgritta M , et al. (2018) |
| Neurophysiology | Synaptic plasticity: post-tetanic potentiation (ptp) | No change | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Increased | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Decreased | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | Decay kinetics of miniature post synaptic currents | Decreased | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Neurophysiology | Synaptic transmission: excitatory | No change | Whole-cell patch clamp | 6-7 weeks | Pea J , et al. (2011) |
| Neurophysiology | Synaptic transmission: excitatory | Abnormal | Whole-cell patch clamp | 6-7 weeks | Pea J , et al. (2011) |
| Neurophysiology | Synaptic transmission | No change | Field potential recordings | 6-7 weeks | Pea J , et al. (2011) |
| Neurophysiology | Synaptic transmission | Decreased | Field potential recordings | 6-7 weeks | Pea J , et al. (2011) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Field potential recordings | 6-7 weeks | Pea J , et al. (2011) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 6-8 weeks | Tatavarty V et al. (2020) |
| Repetitive behavior | Self grooming: perseveration | Increased | Novel cage test | 2-3 months, 6-7 months | Rhine MA , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Repetitive behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 9-10 weeks | Fourie C , et al. (2018) |
| Repetitive behavior | Repetitive digging | Increased | Marble-burying test | NA | Tabouy L , et al. (2018) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 4.5 months, 6 months | Kabitzke PA , et al. (2017) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | NA | Kabitzke PA , et al. (2017) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | Adult | Kabitzke PA , et al. (2017) |
| Repetitive behavior | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Seizure | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Seizure | Seizures | No change | General observations | 5-6 weeks | Pea J , et al. (2011) |
| Sensory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Sensory | Olfaction | No change | Buried food test | 7-10 weeks | Sgritta M , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Sensory | Startle response: acoustic stimulus | Decreased | Acoustic startle reflex test | Adult | Kabitzke PA , et al. (2017) |
| Sensory | Sensorimotor gating | Decreased | Prepulse inhibition | Adult | Kabitzke PA , et al. (2017) |
| Sensory | Olfaction | No change | General observations | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Sensory | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Social behavior | Social memory | decreased | Social recognition test | 3-4 months | Tao K et al. (2022) |
| Social behavior | Social interaction: opposite sex | Decreased | Reciprocal social interaction test | 6-16 weeks | Rhine MA , et al. (2019) |
| Social behavior | Self grooming: social context | Increased | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Social behavior | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Social behavior | Social memory | No change | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 9-10 weeks | Fourie C , et al. (2018) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 9-10 weeks | Fourie C , et al. (2018) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | NA | Tabouy L , et al. (2018) |
| Social behavior | Social scent marking or recognition | No change | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Social behavior | Social memory | No change | Three-chamber social approach test | ~p45 | Kabitzke PA , et al. (2017) |
| Social behavior | Social interaction | Increased | Reciprocal social interaction test | Adult | Kabitzke PA , et al. (2017) |
| Social behavior | Social approach | No change | Three-chamber social approach test | ~p45 | Kabitzke PA , et al. (2017) |
| Social behavior | Social memory | Abnormal | Three-chamber social approach test | 5-6 weeks | Pea J , et al. (2011) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 5-6 weeks | Pea J , et al. (2011) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 5-6 weeks | Pea J , et al. (2011) |
| Physiological parameters | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Decreased | 16s rrna gene sequence analysis for identification of bacteria | 7-10 weeks | Sgritta M , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Decreased | Quantitative pcr (qrt-pcr) | 7-10 weeks | Sgritta M , et al. (2018) |
| Physiological parameters | Digestive system function: intestinal barrier: intestinal permeability | No change | Intestinal permeability test | 7-10 weeks | Sgritta M , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Decreased | Quantitative pcr (qrt-pcr) | 2 months | Tabouy L , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Decreased | 16s rrna gene sequence analysis for identification of bacteria | 2 months | Tabouy L , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Increased | Quantitative pcr (qrt-pcr) | 2 months | Tabouy L , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Decreased | Quantitative pcr (qrt-pcr) | 2 months | Tabouy L , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Decreased | 16s rrna gene sequence analysis for identification of bacteria | 2 months | Tabouy L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Physiological parameters | Hypothermia | No change | Body temperature measurement | P4, p15 | Kabitzke PA , et al. (2017) |
| Physiological parameters | Hypothermia | Decreased | Body temperature measurement | P7 | Kabitzke PA , et al. (2017) |
| Physiological parameters | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Decreased | Monitoring ultrasonic vocalizations | 6-16 weeks | Rhine MA , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | P4,p7, p15 | Kabitzke PA , et al. (2017) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Decreased | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Communications | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Developmental profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Developmental profile | Size/growth | No change | Body weight measurement | P4-p30 | Kabitzke PA , et al. (2017) |
| Developmental profile | Size/growth | Increased | Body weight measurement | P60 | Kabitzke PA , et al. (2017) |
| Developmental profile | Developmental trajectory | No change | Developmental milestone measurements | P4, p7, p13 | Kabitzke PA , et al. (2017) |
| Developmental profile | Size/growth | No change | General observations | Unreported | Pea J , et al. (2011) |
| Developmental profile | Developmental trajectory | Abnormal | General observations | 4-5 months | Pea J , et al. (2011) |
| Emotion | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Emotion | Anxiety | No change | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Emotion | Anxiety | Increased | Light-dark exploration test | 9-10 weeks | Fourie C , et al. (2018) |
| Emotion | Anxiety | Increased | Elevated plus maze test | NA | Tabouy L , et al. (2018) |
| Emotion | Anxiety | Increased | Open field test | NA | Tabouy L , et al. (2018) |
| Emotion | Exploratory activity | Decreased | General observations | NA | Kabitzke PA , et al. (2017) |
| Emotion | Exploratory activity | No change | Three-chamber social approach test | ~p45 | Kabitzke PA , et al. (2017) |
| Emotion | Anxiety | Increased | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Emotion | Anxiety | Increased | Open field test | 5-6 weeks | Pea J , et al. (2011) |
| Emotion | Anxiety | Increased | Light-dark exploration test | 5-6 weeks | Pea J , et al. (2011) |
| Emotion | Anxiety | Increased | Elevated zero maze test | 5-6 weeks | Pea J , et al. (2011) |
| Immune response | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Immune response | Cytokine levels: pro-inflammatory | Increased | Elisa | NA | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: pro-inflammatory | Decreased | Elisa | NA | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: pro-inflammatory | Decreased | Elisa | NA | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: pro-inflammatory | Increased | Elisa | NA | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: anti-inflammatory | Increased | Elisa | NA | Tabouy L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Immune response | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Learning & memory | Swim distance | No change | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Learning & memory | Spatial reference memory | Decreased | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Learning & memory | Spatial learning | Decreased | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Learning & memory | Procedural learning | No change | Procedural water t-maze | Adult | Kabitzke PA , et al. (2017) |
| Learning & memory | Cognitive flexibility | No change | Procedural water t-maze | Adult | Kabitzke PA , et al. (2017) |
| Learning & memory | Spatial reference memory | No change | Morris water maze test | 5-6 weeks | Pea J , et al. (2011) |
| Learning & memory | Object recognition memory | No change | Grooming behavior assessments | 3-6 months | Pea J , et al. (2011) |
| Maternal behavior | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Wang L , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Maternal behavior | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Molecular profile | Signaling: pi3k/akt pathway | No change | Western blot | 0.7, 1.5, 4-5 months | Tai C et al. (2020) |
| Molecular profile | Signaling: mtor pathway | No change | Western blot | 0.7, 1.5, 4-5 months | Tai C et al. (2020) |
| Molecular profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Molecular profile | Protein expression level evidence | No change | Western blot | Adult | Wang L , et al. (2019) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | Adult | Wang L , et al. (2019) |
| Molecular profile | Gene expression | No change | Quantitative pcr (qrt-pcr) | 7-10 weeks | Sgritta M , et al. (2018) |
| Molecular profile | Protein expression: in situ protein expression | No change | Immunohistochemistry | 9-10 weeks | Fourie C , et al. (2018) |
| Molecular profile | Gene expression | Decreased | Quantitative pcr (qrt-pcr) | NA | Tabouy L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 5-6 weeks | Pea J , et al. (2011) |
M_SHANK3_4_KO_HM_COCAINE
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Shank3B null mice were treated with cocaine. Cocaine hydrochloride was dissolved in 0.9% saline and injected I.P. at a volume of 5 ml/kg 24 hr prior to electrophysiological recordings.
Allele Type: Knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: R1
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Neurophysiology | Synaptic plasticity: ventral tegmental area ltp | Ameliorated | Whole-cell patch clamp | 7-10 weeks | Sgritta M , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Social behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
M_SHANK3_4_KO_HM_CX546
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
CX-546 prepared in 25% beta-cyclodextrin in distilled water was injected intraperitoneally 30 min before the start of the behavioral assay on each testing day at a dose of 15mg/kg in Shank3B mutants.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: Not Specified
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | 2-3 months, 6-7 months | Rhine MA , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Novel cage test | 2-3 months, 6-7 months | Rhine MA , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Social behavior | Social interaction: opposite sex | Refractory | Reciprocal social interaction test | 2-3 months | Rhine MA , et al. (2019) |
| Social behavior | Self grooming: social context | Restored | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Refractory | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
M_SHANK3_4_KO_HM_D-CYCLOSERINE-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
320 mg/kg D-cycloserine was administered intraperitoneally, 30 min before the start of the behavioral assay on each testing day in Shank3b mutant mice.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: Not Specified
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Motor phenotype | Hyperactivity | Side effect | Open field test | 2-3 months | Rhine MA , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Restored | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Social behavior | Social interaction: opposite sex | Side effect | Reciprocal social interaction test | 6-16 weeks | Rhine MA , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Side effect | Monitoring ultrasonic vocalizations | 6-16 weeks | Rhine MA , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
M_SHANK3_4_KO_HM_D-CYCLOSERINE-2
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
32 mg/kg D-cycloserine was administered intraperitoneally, 30 min before the start of the behavioral assay on each testing day in Shank3b mutant mice.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: Not Specified
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | 2-3 months | Rhine MA , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Social behavior | Social interaction: opposite sex | Ameliorated | Reciprocal social interaction test | 6-16 weeks | Rhine MA , et al. (2019) |
| Social behavior | Self grooming: social context | Refractory | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Communications | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Learning & memory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
M_SHANK3_4_KO_HM_DHF
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Freshly prepared 7,8-dihydroxyflavon hydrate diluted in DMSO and PBS was administered intraperitoneally subchronically, daily for either 5 days before the start of behavioral testing, and 60 min before the start of the behavioral assay on each testing day, at a dose of 5mg/kg in Shank3B mutant mice.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: Not Specified
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Motor phenotype | Swimming ability | Ameliorated | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | 2-3 months | Rhine MA , et al. (2019) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Repetitive behavior | Self grooming: perseveration | Restored | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Seizure | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Sensory | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Social behavior | Social interaction: opposite sex | Refractory | Reciprocal social interaction test | 2-3 months | Rhine MA , et al. (2019) |
| Social behavior | Self grooming: social context | Refractory | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Physiological parameters | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Refractory | Grooming behavior assessments | 2-3 months | Rhine MA , et al. (2019) |
| Developmental profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Emotion | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Immune response | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Learning & memory | Swim distance | No adverse effect | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Learning & memory | Spatial reference memory | Refractory | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Learning & memory | Spatial learning | Ameliorated | Morris water maze test | 2-3 months | Rhine MA , et al. (2019) |
| Maternal behavior | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
| Molecular profile | NA | Not reported | NA | NA | Rhine MA , et al. (2019) |
M_SHANK3_4_KO_HM_LITHIUM
Model Type: RESCUE-Dietary
Model Genotype: Homozygous
Mutation:
Shank3 mutant mice were put on Li chow diet for 1 week.
Allele Type: Partial knockout
Strain of Origin: NA
Genetic Background: C57Bl6*129svJ
Es cell line: R1
Model Source: Jackson Laboratories
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Repetitive behavior | Self grooming: perseveration | Ameliorated | Grooming behavior assessments | 6-8 weeks | Tatavarty V et al. (2020) |
M_SHANK3_4_KO_HM_LREUTERI
Model Type: RESCUE-Dietary
Model Genotype: Homozygous
Mutation:
Shank3B null mice were treated with either vehicle or L. reuteri, added daily in drinking water for 4 weeks.
Allele Type: Knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: R1
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Motor phenotype | General locomotor activity | Refractory | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: oxytocin expressing | Restored | Immunofluorescence staining | 7-10 weeks | Sgritta M , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | No adverse effect | Immunofluorescence staining | 7-10 weeks | Sgritta M , et al. (2018) |
| Neurophysiology | Synaptic plasticity: ventral tegmental area ltp | Ameliorated | Whole-cell patch clamp | NA | Sgritta M , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Social behavior | Social memory | No adverse effect | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social interaction | Restored | Reciprocal social interaction test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Emotion | Anxiety | No adverse effect | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
M_SHANK3_4_KO_HM_LREUTERI-2
Model Type: RESCUE-Dietary
Model Genotype: Homozygous
Mutation:
Adult shank3 knockout mice were treated for three weeks between ages 8-11 weeks, with L. reuteri. Mice were gavaged with 10^9 bacteria of L. reuteri reconstituted in PBS, twice a week for three weeks. Behavioral experimentation was carried out between ages 11-13 weeks, and then mice were immediately sacrificed for downstream molecular and biochemical analysis. L. reuteri used for this study was obtained from ATCC (Lactobacillus reuteri MM4-1A; ATCC-PTA-6475).
Allele Type: Knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified
Es cell line: -
Model Source: PMID 21423165
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: gaba-r: gabaa | Ameliorated | Western blot | 3 months | Tabouy L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: gaba-r: gabaa | Ameliorated | Quantitative pcr (qrt-pcr) | 3 months | Tabouy L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Repetitive behavior | Repetitive digging | Ameliorated | Marble-burying test | 3 months | Tabouy L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Social behavior | Social approach | Ameliorated | Three-chamber social approach test | 3 months | Tabouy L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 3 months | Tabouy L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Physiological parameters | Intestinal microbiota composition | Restored | Quantitative pcr (qrt-pcr) | 3 months | Tabouy L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Emotion | Anxiety | Refractory | Elevated plus maze test | 3 months | Tabouy L , et al. (2018) |
| Emotion | Anxiety | Refractory | Open field test | 3 months | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: pro-inflammatory | Restored | Elisa | 3 months | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: pro-inflammatory | Refractory | Elisa | 3 months | Tabouy L , et al. (2018) |
| Immune response | Cytokine levels: anti-inflammatory | Restored | Elisa | 3 months | Tabouy L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Tabouy L , et al. (2018) |
| Molecular profile | Gene expression | Ameliorated | Quantitative pcr (qrt-pcr) | 3 months | Tabouy L , et al. (2018) |
| Molecular profile | Gene expression | Refractory | Quantitative pcr (qrt-pcr) | 3 months | Tabouy L , et al. (2018) |
M_SHANK3_4_KO_HM_OXYTOCIN
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Shank3B null mice were treated with oxytocin, intranasally at a dose of 200mg/kg dissolved in 10% DMSO in PBS.
Allele Type: Knockout
Strain of Origin: Not Specified
Genetic Background: C57Bl6*129svJ
Es cell line: R1
Model Source: JAX
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Motor phenotype | General locomotor activity: ambulatory activity | Refractory | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Motor phenotype | General locomotor activity | Refractory | Open field test | 7-10 weeks | Sgritta M , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Neurophysiology | Synaptic plasticity: ventral tegmental area ltp | Ameliorated | Whole-cell patch clamp | 7-10 weeks | Sgritta M , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Social behavior | Social memory | No adverse effect | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social interaction | Restored | Reciprocal social interaction test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
M_SHANK3_4_KO_HM_ZINC
Model Type: RESCUE-Dietary
Model Genotype: Homozygous
Mutation:
M_SHANK3_4_KO_HM mice wereassigned to a zinc supplementation diet (150 ppm) for 6 weeks from weaning (P21), compared to a normal zinc diet (30 ppm)
Allele Type: Knockout
Strain of Origin: Not reported
Genetic Background: C57Bl6*129svJ
Es cell line: Not reported
Model Source: Jackson Laboratories, Bar Harbor, ME, USA
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Motor phenotype | General locomotor activity: ambulatory activity | Restored | Home cage behavior | 9-10 weeks | Fourie C , et al. (2018) |
| Motor phenotype | General locomotor activity | Restored | Home cage behavior | 9-10 weeks | Fourie C , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Neurophysiology | Synaptic plasticity: post-tetanic potentiation (ptp) | Side effect | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Side effect | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | Refractory | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Neurophysiology | Decay kinetics of miniature post synaptic currents | Restored | Whole-cell patch clamp | 9-10 weeks | Fourie C , et al. (2018) |
| Repetitive behavior | Self grooming: perseveration | Restored | Grooming behavior assessments | 9-10 weeks | Fourie C , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Social behavior | Social memory | Restored | Three-chamber social approach test | 9-10 weeks | Fourie C , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 9-10 weeks | Fourie C , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Emotion | Anxiety | Restored | Light-dark exploration test | 9-10 weeks | Fourie C , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Fourie C , et al. (2018) |
| Molecular profile | Protein expression: in situ protein expression | Side effect | Immunohistochemistry | 9-10 weeks | Fourie C , et al. (2018) |
M_SHANK3_4_KO_HT
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exon 13 to 16 of Shank3 containing the PDZ domain, the positive selection marker neo cassette is retained.
Allele Type: Partial knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J*129Sv/Ev
Es cell line: R1
Model Source: PMID 21423165
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Sensory | Touch | Increased | Response to air puff | 6-8 weeks | Orefice LL , et al. (2016) |
| Sensory | Tactile memory | Decreased | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2016) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 6-8 weeks | Orefice LL , et al. (2016) |
| Sensory | Sensorimotor gating: tactile cue | Decreased | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2016) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 6-8 weeks | Orefice LL , et al. (2016) |
| Social behavior | Social memory | No change | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 7-10 weeks | Sgritta M , et al. (2018) |
| Social behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Emotion | Exploratory activity | No change | Textured novel object recognition test (t-nort) | 6-8 weeks | Orefice LL , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Learning & memory | Object recognition memory: long-term recall | Decreased | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2016) |
| Learning & memory | Object recognition memory | No change | Novel object recognition test | 6-8 weeks | Orefice LL , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
| Molecular profile | NA | Not reported | NA | NA | Sgritta M , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Orefice LL , et al. (2016) |
M_SHANK3_5_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exons 4 - 7 containing the ankyrin repeat domains of Shank3 gene, the positive selection marker neo cassette is retained.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified
Es cell line: R1
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Motor phenotype | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Neurophysiology | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Repetitive behavior | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Seizure | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Sensory | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Social behavior | Social memory | Abnormal | Three-chamber social approach test | Unreported | Pea J , et al. (2011) |
| Social behavior | Social approach | No change | Three-chamber social approach test | Unreported | Pea J , et al. (2011) |
| Physiological parameters | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Communications | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Developmental profile | Size/growth | No change | General observations | Unreported | Pea J , et al. (2011) |
| Developmental profile | Developmental trajectory | No change | General observations | 3-6 months | Pea J , et al. (2011) |
| Emotion | Anxiety | No change | Open field test | Unreported | Pea J , et al. (2011) |
| Emotion | Anxiety | No change | Light-dark exploration test | Unreported | Pea J , et al. (2011) |
| Emotion | Anxiety | No change | Elevated zero maze test | Unreported | Pea J , et al. (2011) |
| Immune response | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Learning & memory | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Maternal behavior | NA | Not reported | NA | NA | Pea J , et al. (2011) |
| Molecular profile | NA | Not reported | NA | NA | Pea J , et al. (2011) |
M_SHANK3_6_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exons 4-9 of Shank3 gene which includes the ankyrin repeat domains.
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified
Es cell line: 129SvEv
Model Source: Not Specified
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | Righting response | No change | Righting reflex test | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Paw preference | No change | General observations | P30 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Paw preference | Decreased | Paw preference test | P60 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Negative geotaxis | No change | Negative geotaxis test | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Motor phenotype | General locomotor activity: ambulatory activity | Decreased | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Motor phenotype | General locomotor activity | No change | Marble-burying test | Adult | Kabitzke PA , et al. (2017) |
| Motor phenotype | Gait | No change | General observations | P30, p60 | Kabitzke PA , et al. (2017) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | Unreported | Wang X , et al. (2011) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | Unreported | Wang X , et al. (2011) |
| Motor phenotype | Motor coordination and balance | Decreased | Footslip test | Unreported | Wang X , et al. (2011) |
| Motor phenotype | Grip strength | Decreased | Vertical pole test | Unreported | Wang X , et al. (2011) |
| Motor phenotype | General locomotor activity | Decreased | Open field test | Unreported | Wang X , et al. (2011) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Neurophysiology | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 4.5 months, 6 months | Kabitzke PA , et al. (2017) |
| Repetitive behavior | Repetitive digging | No change | Marble-burying test | Adult | Kabitzke PA , et al. (2017) |
| Repetitive behavior | Head bobbing | Increased | Hole-board test | Unreported | Wang X , et al. (2011) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Seizure | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | Adult | Kabitzke PA , et al. (2017) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | Adult | Kabitzke PA , et al. (2017) |
| Sensory | Olfaction | No change | General observations | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Sensory | Olfaction | No change | Urine preference test | Unreported | Wang X , et al. (2011) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Social behavior | Social scent marking or recognition | No change | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Social behavior | Social memory | No change | Three-chamber social approach test | ~p45 | Kabitzke PA , et al. (2017) |
| Social behavior | Social interaction | No change | Reciprocal social interaction test | ~p45 | Kabitzke PA , et al. (2017) |
| Social behavior | Social approach | No change | Three-chamber social approach test | ~p45 | Kabitzke PA , et al. (2017) |
| Social behavior | Social interaction | Decreased | Reciprocal social interaction test | 3-6 months | Wang X , et al. (2011) |
| Social behavior | Social approach | No change | Three-chamber social approach test | 3-6 months | Wang X , et al. (2011) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Physiological parameters | Hypothermia | No change | Body temperature measurement | P4, p7, p15 | Kabitzke PA , et al. (2017) |
| Physiological parameters | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | Ultrasonic vocalization: isolation induced | No change | Monitoring ultrasonic vocalizations | P4,p7, p15 | Kabitzke PA , et al. (2017) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | Decreased | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Communications | Ultrasonic vocalization | Abnormal | Monitoring ultrasonic vocalizations | 3-4 months | Wang X , et al. (2011) |
| Communications | Ultrasonic vocalization | Abnormal | Monitoring ultrasonic vocalizations | 3-4 months | Wang X , et al. (2011) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | Size/growth | No change | Body weight measurement | P4-p90 | Kabitzke PA , et al. (2017) |
| Developmental profile | Developmental trajectory | No change | Developmental milestone measurements | P4, p7, p13 | Kabitzke PA , et al. (2017) |
| Developmental profile | Size/growth | No change | General observations | 3-4 months | Wang X , et al. (2011) |
| Developmental profile | Size/growth | Increased | General observations | 8-12 months | Wang X , et al. (2011) |
| Developmental profile | General characteristics | No change | General observations | Unreported | Wang X , et al. (2011) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | Exploratory activity | Decreased | Three-chamber social approach test | ~p45 | Kabitzke PA , et al. (2017) |
| Emotion | Anxiety | Increased | Open field test in presence of female urine | Adult | Kabitzke PA , et al. (2017) |
| Emotion | Response to novelty | No change | Open field test | Unreported | Wang X , et al. (2011) |
| Emotion | Response to novelty | No change | Zero maze test | Unreported | Wang X , et al. (2011) |
| Emotion | Anxiety | No change | Light-dark exploration test | Unreported | Wang X , et al. (2011) |
| Emotion | Anxiety | No change | Elevated zero maze test | Unreported | Wang X , et al. (2011) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Immune response | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | Procedural learning | No change | Procedural water t-maze | Adult | Kabitzke PA , et al. (2017) |
| Learning & memory | Cognitive flexibility | No change | Procedural water t-maze | Adult | Kabitzke PA , et al. (2017) |
| Learning & memory | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Maternal behavior | NA | Not reported | NA | NA | Wang X , et al. (2011) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein localization: nucleus | Increased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Increased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Kabitzke PA , et al. (2017) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | Unreported | Wang X , et al. (2011) |
| Molecular profile | Gene expression | Abnormal | Western blot | Unreported | Wang X , et al. (2011) |
M_SHANK3_6_KO_HM_ROMIDEPSIN-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Systemic administration of low-dose romidepsin (0.25mg/kg, intraperitoneally (i.p.), once daily for 3 d), a highly potent and brain-permeable class I-specific HDAC inhibitor, to mice bearing homozygous deletion of exons 4-9. .
Allele Type: Partial knockout
Strain of Origin: Not Specified
Genetic Background: Not Specified
Es cell line: 129SvEv
Model Source: PMID 21558424
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 5-6 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_7_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exon 11 within the SH3 domain of Shank3 gene. A loxP site was inserted upstream of exon 11 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 11 via homologous recombination. Recombinase-mediated recombination deleted exon 11 and the neo cassette. Deletion allele also known as Shank3^alphabeta- or Shank3delta11-.
Allele Type: Knockout
Strain of Origin: Not specified
Genetic Background: C57BL/6
Es cell line: Not specified
Model Source: Tobias Boeckers lab, Genoway
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Motor phenotype | Grip strength | Decreased | Wire hang test | 4 weeks | Lutz AK et al. (2020) |
| Motor phenotype | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Number of oligodendrocytes | Decreased | Immunohistochemistry | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal morphology: axonal structure | No change | Transmission electron microscopy (TEM) | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination: PNS | Increased | G-ratio measurement (axon diameters/fiber diameters of myelinated axons) | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination: PNS | Increased | Fluorescence microscopy | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination: PNS | Decreased | Immunohistochemistry | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination: PNS | Increased | Western blot | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination: PNS | Increased | Immunohistochemistry | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination: Node of Ranvier morphology | Abnormal | Immunohistochemistry | 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | Decreased | G-ratio measurement (axon diameters/fiber diameters of myelinated axons) | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | No change | G-ratio measurement (axon diameters/fiber diameters of myelinated axons) | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | Increased | Western blot | 1 week | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | Increased | Immunohistochemistry | 1 week | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | Decreased | Western blot | 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | Decreased | Immunohistochemistry | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Myelination | Decreased | Fluorescence microscopy | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of the basal ganglia: ventral striatum or nucleus accumbens | Decreased | Fluorescence microscopy | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Magnetic resonance imaging (MRI) | 4 weeks, 9 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Immunohistochemistry | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology and size of the corpus callosum | Decreased | Fluorescence microscopy | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Cortical thickness | No change | Immunohistochemistry | 20 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | Decreased | Magnetic resonance imaging (MRI) | 4 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Brain size | No change | Magnetic resonance imaging (MRI) | 9 weeks | Malara M et al. (2022) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology | Decreased | Immunofluorescence staining | 1.9 months | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synapse density: Excitatory | Decreased | Immunofluorescence staining | 1.9 months | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: acetylcholine | Decreased | Immunofluorescence staining | P0-1, 1.9 months | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Motor neuron morphology | Decreased | Immunofluorescence staining | 1.9 months | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of Z-disc | Decreased | Electron microscopy | P0-1, 5 weeks, 1.9 months | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of neuromuscular junction | Abnormal | Electron microscopy | 1.9 months, Adult | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Morphology of A band | Increased | Electron microscopy | P0-1, 5 weeks, 1.9 months | Lutz AK et al. (2020) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | 3-6 months | Heise C , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 3-6 months | Heise C , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | Decreased | Western blot | 3-6 months | Heise C , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 3-6 months | Heise C , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: gaba-r: gabaa | No change | Western blot | 3-6 months | Heise C , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology | No change | Electron microscopy | Unreported | Schmeisser MJ , et al. (2012) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | No change | Golgi-cox staining | Unreported | Schmeisser MJ , et al. (2012) |
| Neurophysiology | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Repetitive behavior | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Seizure | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Sensory | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Social behavior | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Social behavior | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Physiological parameters | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Communications | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Developmental profile | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Emotion | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Immune response | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Learning & memory | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Maternal behavior | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Schmeisser MJ , et al. (2012) |
| Molecular profile | Targeted expression | Decreased | Immunohistochemistry | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Molecular profile | Targeted expression | Increased | Western blot | 1 week | Malara M et al. (2022) |
| Molecular profile | Targeted expression | Decreased | Western blot | 1 week, 3 weeks, 20 weeks | Malara M et al. (2022) |
| Molecular profile | Targeted expression | Decreased | Immunofluorescence staining | 1.9 months, P0, P1, Adult | Lutz AK et al. (2020) |
| Molecular profile | Targeted expression | Decreased | Co-immunoprecipitation | 1.9 months, P0, P1, Adult | Lutz AK et al. (2020) |
| Molecular profile | NA | Not reported | NA | NA | Heise C , et al. (2018) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | Unreported | Schmeisser MJ , et al. (2012) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | Unreported | Schmeisser MJ , et al. (2012) |
| Molecular profile | Gene expression | No change | Semi-quantitative pcr (qrt-pcr) | Unreported | Schmeisser MJ , et al. (2012) |
M_SHANK3_7_KO_HM_TIRASEMTIV
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Mice with targeted deletion of exon 11 within the sh3 domain of shank3 were treated with tirasemtiv. 30 days old wild type and shank3del11(-/-) mice received intraperitoneal injection of 100 microliter tirasemtiv or ck2017357 (10
Allele Type: -
Strain of Origin: -
Genetic Background: -
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Motor phenotype | Grip strength | Restored | Wire hang test | 4 weeks | Lutz AK et al. (2020) |
M_SHANK3_8_KO_HM
Model Type: Genetic
Model Genotype: Homozygous
Mutation:
Targeted deletion of exon 21 of the Shank3 gene using cre/loxP and frt flanked neo cassette. After removal of the neo cassette, mice containing the floxed gene were mated to actin-Cre mice for deleting the exon. Wild type Shank3 protein is not detected in these mice. Lower molecular weight isoforms of Shank3 are however detectable.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | Sleep pattern: total nighttime sleep | Decreased | Electroencephalogram/electromyogram (eeg/emg) | 12 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Sleep pattern: total daytime sleep | No change | Electroencephalogram/electromyogram (eeg/emg) | 12 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Sleep pattern: sleep recovery | Decreased | Electroencephalogram/electromyogram (eeg/emg) | 9, 11 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Sleep pattern: sleep latency | Increased | Electroencephalogram/electromyogram (eeg/emg) | 9, 11 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Sleep pattern: number of sleep bouts | Decreased | Electroencephalogram/electromyogram (eeg/emg) | 12 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Rapid eye movement sleep (rems) | Decreased | Electroencephalogram/electromyogram (eeg/emg) | 12 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Non rapid eye movement sleep (nrems) | Decreased | Electroencephalogram/electromyogram (eeg/emg) | 12 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | Circadian rhythms: timing/phases of locomotor activity | Decreased | Wheel running monitoring | 9, 11 weeks | Ingiosi AM , et al. (2019) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Circadian sleep/wake cycle | Sleep pattern: sleep bout length | Decreased | Electroencephalogram/electromyogram (eeg/emg) | 12 weeks | Kouser M , et al. (2013) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
| Motor phenotype | Rearing behavior | No change | Novel cage test | 2-3 months | Krüttner S et al. (2022) |
| Motor phenotype | Rearing behavior | Decreased | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Novel cage test | 2-3 months | Krüttner S et al. (2022) |
| Motor phenotype | General locomotor activity: ambulatory activity | No change | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Motor phenotype | Motor coordination and balance | No change | Balance beam test | P50-60 | Matas E et al. (2021) |
| Motor phenotype | Motor coordination and balance | Decreased | Balance beam test | P50-60 | Matas E et al. (2021) |
| Motor phenotype | Gait | Abnormal | Gait | P50-60 | Matas E et al. (2021) |
| Motor phenotype | General locomotor activity | No change | Open field test | Adult | Bidinosti M , et al. (2016) |
| Motor phenotype | General locomotor activity | No change | Accelerating rotarod test | Adult | Bidinosti M , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | Motor coordination and balance | Decreased | Accelerating rotarod test | 2-6 months | Kouser M , et al. (2013) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: AMPA receptors | No change | Quantitative PCR (qRT-PCR) | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: AMPA receptors | No change | Western blot | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: AMPA receptors | Decreased | Western blot | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: Purkinje cells | Decreased | Immunostaining | P50-60 | Matas E et al. (2021) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic neuroreceptors | Increased | Western blot | 5-6 months | Kouser M , et al. (2013) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 5-6 months | Kouser M , et al. (2013) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 5-6 months | Kouser M , et al. (2013) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | No change | Sholl analysis | P13-17, 6-8 months | Kouser M , et al. (2013) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | No change | Sholl analysis | P13-17, 6-8 months | Kouser M , et al. (2013) |
| Neurophysiology | Neurotransmitter release: catecholamines: dopamine | Increased | Fiber photometry | 2-3 months | Krüttner S et al. (2022) |
| Neurophysiology | Event related oscillations (eros) in electroencephalography (eeg) | Abnormal | Electroencephalogram (eeg) | 9, 11 weeks | Ingiosi AM , et al. (2019) |
| Neurophysiology | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission | Decreased | Field potential recordings | P13-17 | Kouser M , et al. (2013) |
| Neurophysiology | Synaptic plasticity: hippocampal ltp | Decreased | Field potential recordings | 3-4 months | Kouser M , et al. (2013) |
| Neurophysiology | Synaptic plasticity: hippocampal ltd | No change | Field potential recordings | 6-8 weeks, 3-4 weeks | Kouser M , et al. (2013) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | 3-4 months | Kouser M , et al. (2013) |
| Neurophysiology | Presynaptic function: paired-pulse facilitation | No change | Whole-cell patch clamp | P13-17 | Kouser M , et al. (2013) |
| Neurophysiology | Miniature post synaptic current frequency: excitatory | Decreased | Whole-cell patch clamp | P13-17 | Kouser M , et al. (2013) |
| Neurophysiology | Miniature post synaptic current amplitude: excitatory | No change | Whole-cell patch clamp | P13-17 | Kouser M , et al. (2013) |
| Repetitive behavior | Stereotypy | No change | Novel cage test | 2-3 months | Krüttner S et al. (2022) |
| Repetitive behavior | Stereotypy | Increased | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Repetitive behavior | Self injurious behavior | Increased | General observations | 3 months | Krüttner S et al. (2022) |
| Repetitive behavior | Self grooming | No change | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Repetitive behavior | Self grooming | No change | Novel cage test | 2-3 months | Krüttner S et al. (2022) |
| Repetitive behavior | Self grooming | Increased | Grooming behavior assessments | P50-60 | Matas E et al. (2021) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | Adult | Bidinosti M , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 2-4.5 months | Kouser M , et al. (2013) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 10-13 months | Kouser M , et al. (2013) |
| Repetitive behavior | Repetitive digging | Decreased | Marble-burying test | 2-6 months | Kouser M , et al. (2013) |
| Seizure | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
| Sensory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | Startle response: acoustic stimulus | No change | Acoustic startle reflex test | 2-6 months | Kouser M , et al. (2013) |
| Sensory | Sensorimotor gating | No change | Prepulse inhibition | 2-6 months | Kouser M , et al. (2013) |
| Sensory | Pain or nociception | Increased | Hot plate test | 2-6 months | Kouser M , et al. (2013) |
| Social behavior | Social memory: long-term social memory | Decreased | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social memory | No change | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social approach | Decreased | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social approach | No change | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | P50-60 | Matas E et al. (2021) |
| Social behavior | Social approach | No change | Three-chamber social approach test | P50-60 | Matas E et al. (2021) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | Adult | Bidinosti M , et al. (2016) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Social behavior | Social memory | Decreased | Three-chamber social approach test | 2-6 months | Kouser M , et al. (2013) |
| Social behavior | Social interaction | No change | Reciprocal social interaction test | 2-6 months | Kouser M , et al. (2013) |
| Social behavior | Social approach | No change | Three-chamber social approach test | 2-6 months | Kouser M , et al. (2013) |
| Social behavior | Nest building behavior | Decreased | Nest building assay | 2-6 months | Kouser M , et al. (2013) |
| Social behavior | Inanimate object preference | Decreased | Three-chamber social approach test | 2-6 months | Kouser M , et al. (2013) |
| Physiological parameters | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
| Communications | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | Ultrasonic vocalization: interaction induced: opposite sex stimulus | No change | Monitoring ultrasonic vocalizations | 10-13 months | Kouser M , et al. (2013) |
| Developmental profile | Mortality/lethality | No change | General observations | unreported | Krüttner S et al. (2022) |
| Developmental profile | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
| Emotion | Exploratory activity | Decreased | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Emotion | Anxiety | Abnormal | Elevated pluz maze test | 2-3 months | Krüttner S et al. (2022) |
| Emotion | Exploratory activity | Decreased | Marble-burying test | Adult | Bidinosti M , et al. (2016) |
| Emotion | Anxiety | No change | Elevated zero maze test | Adult | Bidinosti M , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | Response to novelty | Decreased | Open field test | 2-6 months | Kouser M , et al. (2013) |
| Emotion | Anxiety | Increased | Light-dark exploration test | 2-6 months | Kouser M , et al. (2013) |
| Emotion | Anxiety | No change | Open field test | 2-6 months | Kouser M , et al. (2013) |
| Emotion | Anxiety | No change | Elevated plus maze test | 2-6 months | Kouser M , et al. (2013) |
| Immune response | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
| Learning & memory | Object recognition memory: long-term recall | Decreased | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | Object recognition memory | No change | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | Spatial reference memory | Decreased | Morris water maze test | 2-6 months | Kouser M , et al. (2013) |
| Learning & memory | Spatial learning | Decreased | Morris water maze test | 2-6 months | Kouser M , et al. (2013) |
| Learning & memory | Cognitive flexibility | No change | Morris water maze test | 2-6 months | Kouser M , et al. (2013) |
| Maternal behavior | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
| Molecular profile | Targeted expression | Decreased | General observations | unreported | Krüttner S et al. (2022) |
| Molecular profile | Gene expression | Increased | Rna sequencing | 8-10 weeks | Ingiosi AM , et al. (2019) |
| Molecular profile | Gene expression | Decreased | Rna sequencing | 8-10 weeks | Ingiosi AM , et al. (2019) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | E16 | Bidinosti M , et al. (2016) |
| Molecular profile | Targeted expression | Decreased | Western blot | NA | Duffney LJ , et al. (2015) |
| Molecular profile | NA | Not reported | NA | NA | Kouser M , et al. (2013) |
M_SHANK3_8_KO_HM_6-OHDA
Model Type: RESCUE-Procedural
Model Genotype: Homozygous
Mutation:
Ablation of dopaminergic axon terminals by local injection of 6-hydroxydopamine (6-OHDA) in Shank3 mutant mice and wildtype littermates. Ablation of dopaminergic axon terminals in tail of striatum (TS) is confirmed by loss of tyrosine hydroxylase (TH) labeling following local injection of 6-hydroxydopamine.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Social behavior | Social approach | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | Object recognition memory: long-term recall | Restored | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
M_SHANK3_8_KO_HM_EE
Model Type: RESCUE-Behavioral
Model Genotype: Homozygous
Mutation:
Wildtype and Shank3 mutant mice were subjected to Environmental Enrichement (EE) for 3 weeks. Male mice were housed in groups of four to five littermates in large (rat) cages equipped with three running wheels per cage, toys, and hiding spaces. Toys and running wheels were frequently (every 4 to 7 days) replaced by novel and different toys or running wheels. Control mice were male littermates (four to five), which were housed individually in small standard cages without special equipment. Unless stated otherwise, EE was started at 1 month of age and lasted 21 days.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Repetitive behavior | Stereotypy | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social approach | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Emotion | Exploratory activity | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | Object recognition memory: long-term recall | Restored | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
M_SHANK3_8_KO_HM_FAM
Model Type: RESCUE-Behavioral
Model Genotype: Homozygous
Mutation:
Wildtype and Shank3 mutant mice were subjected to a familiar feature during initial 10 minutes context exposure, either home cage bedding or familiar objects that had been in the home cage for 10 days
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Neurophysiology | Neurotransmitter release: catecholamines: dopamine | Ameliorated | Fiber photometry | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social memory: long-term social memory | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Social behavior | Social approach | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | Object recognition memory: long-term recall | Restored | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
M_SHANK3_8_KO_HM_PREL-TS-ACT
Model Type: RESCUE-Procedural
Model Genotype: Homozygous
Mutation:
Floxed PSAM-carrying AAV9 (excitation, pAAV(9)-pCAG-flox-PSAM(Leu41Phe,Tyr116Phe)5HT3-WPRE (Vector Biosystems Inc) was delivered bilaterally to the prelimbic cortex (PreL) and a retro AAV delivering the Cre recombinase (rgAAV-Ef1a-mCherry-IRES-Cre, Addgene 55632-AAVrg) was delivered bilaterally to TS. PSEM308 channel agonist (ligand, Apex Scientific) was injected intraperitoneal at 5 mg per kg of animal weight. Agonist injections were carried out after initial context exposure, 20 min before the start of the behavioral procedure (re-exposure).
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Social behavior | Social approach | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
M_SHANK3_8_KO_HM_SCH-23390-PREL
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Mice were locally treated with D1/5R antagonist [SCH-23390 (1.5mM in saline per side, Sigma; D1/5 dopamine receptor antagonist)] in the prelimbic cortex (PreL) 12 h after initial context exposure to prevent long-term memory consolidation at indicated brain areas bilaterally
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Social behavior | Social approach | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | Object recognition memory: long-term recall | Restored | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
M_SHANK3_8_KO_HM_SCH-23390-TS
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
Mice were locally treated with D1/5R antagonist [SCH-23390 (1.5mM in saline per side, Sigma; D1/5 dopamine receptor antagonist)] in tail of striatum (TS) 12 h after initial context exposure to prevent long-term memory consolidation at indicated brain areas bilaterally
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: Paul F. Worley Lab (PMID 21565394)
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Social behavior | Social approach | Restored | Interaction with caged adult | 2-3 months | Krüttner S et al. (2022) |
| Learning & memory | Object recognition memory: long-term recall | Restored | Novel object recognition test | 2-3 months | Krüttner S et al. (2022) |
M_SHANK3_8_KO_HM_TG003
Model Type: RESCUE-Pharmaceutical
Model Genotype: Homozygous
Mutation:
SHANK3: ablation of exon 21 (previously described in PMID 24259569); Deletion of exon 21 removes the majority of the Shank3 C-terminus; major isoforms absent but fast migrating truncated fragments present; mice were treated by IP injection with 30mg/kg TG003, CLK2 inhibitor.
Allele Type: Knockout
Strain of Origin: C57Bl/6
Genetic Background: C57BL/6J
Es cell line: C57BL/6J ES cells
Model Source: Generated by the authors of PMID26847545
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Repetitive behavior | Self grooming: perseveration | Ameliorated | Grooming behavior assessments | Adult | Bidinosti M , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Social behavior | Social approach | Sustained effect | Three-chamber social approach test | Adult | Bidinosti M , et al. (2016) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | Adult | Bidinosti M , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Emotion | Exploratory activity | Refractory | Marble-burying test | Adult | Bidinosti M , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Molecular profile | Protein phosphorylation | Restored | Western blot | E16 | Bidinosti M , et al. (2016) |
M_SHANK3_8_KO_HT
Model Type: Genetic
Model Genotype: Heterozygous
Mutation:
Targeted deletion of exon 21 (previously described in PMID 24259569); Deletion of exon 21 removes the majority of the Shank3 C-terminus; major isoforms absent but fast migrating truncated fragments present.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 5-6 weeks | Qin L , et al. (2018) |
| Motor phenotype | General locomotor activity | No change | Novel cage test | 5-6 weeks | Qin L , et al. (2018) |
| Motor phenotype | General locomotor activity | No change | Open field test | Adult | Bidinosti M , et al. (2016) |
| Motor phenotype | General locomotor activity | No change | Accelerating rotarod test | Adult | Bidinosti M , et al. (2016) |
| Motor phenotype | Gait | No change | Running stride analysis | 2-4 months | Kloth AD , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No change | Accelerating rotarod test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Motor phenotype | General locomotor activity | No change | Open field test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology | No change | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | No change | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal morphology | No change | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number: purkinje cells | No change | Histology | 2-4 months | Kloth AD , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | Decreased | Sholl analysis | 2-4 months | Kloth AD , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: dendritic tree complexity | Increased | Sholl analysis | 2-4 months | Kloth AD , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Cerebellar morphology: molecular layer thickness | No change | Histology | 2-4 months | Kloth AD , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Cerebellar morphology: granule cell layer thickness | No change | Histology | 2-4 months | Kloth AD , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synapse density | No change | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No change | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Decreased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No change | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Dendritic architecture: spine density | No change | Sholl analysis | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Neurophysiology | Epsp-spike relationship | Decreased | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Neurophysiology | Epsp-spike relationship | No change | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | No change | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Decreased | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | No change | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Decreased | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | No change | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | No change | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Decreased | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 5-6 weeks | Qin L , et al. (2018) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | Adult | Bidinosti M , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | Increased | Grooming behavior assessments | 6-8 weeks | Duffney LJ , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | No change | Grooming behavior assessments | 6-8 weeks | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 5-6 weeks | Qin L , et al. (2018) |
| Social behavior | Social approach | No change | Three-chamber social approach test | Adult | Bidinosti M , et al. (2016) |
| Social behavior | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Social behavior | Social memory | No change | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Decreased | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | No change | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | Anxiety | No change | Open field test | 5-6 weeks | Qin L , et al. (2018) |
| Emotion | Exploratory activity | Decreased | Marble-burying test | Adult | Bidinosti M , et al. (2016) |
| Emotion | Anxiety | No change | Elevated zero maze test | Adult | Bidinosti M , et al. (2016) |
| Emotion | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Emotion | Anxiety | No change | Open field test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Learning & memory | Spatial learning | No change | Water y-maze test | 2-4 months | Kloth AD , et al. (2015) |
| Learning & memory | Eye blink conditioning: extinction and reactivation | No change | Eyeblink conditioning | 2-4 months | Kloth AD , et al. (2015) |
| Learning & memory | Eye blink conditioning | Decreased | Eyeblink conditioning | 2-4 months | Kloth AD , et al. (2015) |
| Learning & memory | Eye blink conditioning | Decreased | Eyeblink conditioning | NA | Kloth AD , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Molecular profile | Targeted expression | Decreased | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein-dna complex assembly | No change | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein-dna complex assembly | Increased | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein localization: nucleus | Increased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein localization: cytosol | Increased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | No change | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Decreased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | No change | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | No change | Western blot | 5-8 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Increased | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Decreased | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No change | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No change | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No change | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Increased | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Abnormal | Rna sequencing | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Side effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Cytoskeletal organization | Decreased | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | No change | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | No change | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | Decreased | Western blot | 5-8 weeks | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Molecular profile | NA | Not reported | NA | NA | Kloth AD , et al. (2015) |
| Molecular profile | Signaling | Decreased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Protein phosphorylation | Decreased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Protein localization: synapse | Decreased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Enzyme activity | Decreased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Enzyme activity | Increased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | No change | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Decreased | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | No change | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Decreased | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Decreased | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
M_SHANK3_8_KO_HT_ARIPIPRAZOLE
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of Aripiprazole once daily for 3 d in mice bearing a heterozygous ablation of exon 21. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_CA-RAC1
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Shank3 het mice were injected with a constitutively active form of Rac1 conjugated to the herpes simplex viral (HSV) genome, directly into the prefrontal cortex. (the authors use GFP inserted into the HSV genome as the control injections).
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Ameliorated | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Repetitive behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Ameliorated | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Molecular profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
M_SHANK3_8_KO_HT_CLOZAPINE
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of atypical antipsychotic clozapine (once daily for 3 d) in mice bearing a heterozygous ablation of exon 21. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_CTNNB1_KD
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Shank3 with heterozygous ablation of exon 21 and stereotaxic injection of beta-catenin shRNA or a scrambled control shRNA lentivirus into the Prefrontal cortex. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 5-6 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Ameliorated | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Ameliorated | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Ameliorated | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Ameliorated | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_FLUOXETINE
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of selective serotonin reuptake inhibitor (SSRI) antidepressant fluoxetine (once daily for 14 d) in mice bearing a heterozygous ablation of exon 21. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | Epsp-spike relationship | Refractory | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_HDAC2_KD-1
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Knockdown of HDAC2 by stereotaxic injection of HDAC2 short hairpin RNA (shRNA-1) lentivirus in the medial PFC in mice with exon 21 ablation. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | Epsp-spike relationship | Restored | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 5-6 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Restored | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Restored | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No adverse effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_HDAC2_KD-2
Model Type: RESCUE-Genetic
Model Genotype: Heterozygous
Mutation:
Knockdown of HDAC2 by stereotaxic injection of HDAC2 short hairpin RNA (shRNA-2) lentivirus in the medial PFC in mice with exon 21 ablation. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 5-6 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | No adverse effect | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Restored | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No adverse effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_RISPERIDONE
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of Risperidone once daily for 3 d in mice bearing a heterozygous ablation of exon 21. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_ROMIDEPSIN-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of low-dose romidepsin (0.25mg/kg, intraperitoneally (i.p.), once daily for 3 d), a highly potent and brain-permeable class I-specific HDAC inhibitor, to mice bearing a heterozygous ablation of exon 21. This is equivalent to ~5% of the clinical anticancer dose in humans. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | Motor coordination and balance | No adverse effect | Accelerating rotarod test | 5-6 weeks | Qin L , et al. (2018) |
| Motor phenotype | General locomotor activity | Refractory | Novel cage test | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Synaptic morphology | No adverse effect | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | No adverse effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: nmda receptors | Side effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuroreceptor levels: glutamate receptors: ampa receptors | No adverse effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal number | No adverse effect | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Neuroanatomy / Ultrastructure / Cytoarchitecture | Neuronal morphology | No adverse effect | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Restored | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Neurophysiology | Epsp-spike relationship | No adverse effect | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Neurophysiology | Epsp-spike relationship | Restored | Whole-cell voltage clamp | 5-6 weeks | Qin L , et al. (2018) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Grooming behavior assessments | 5-6 weeks | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 5-6 weeks | Qin L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | Adult | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | Anxiety | No adverse effect | Open field test | 5-6 weeks | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | Targeted expression | Refractory | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | No adverse effect | Western blot | 5-8 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Restored | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | No adverse effect | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Protein expression level evidence | Side effect | Western blot | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Ameliorated | Rna sequencing | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No adverse effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Side effect | Rna sequencing | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | No adverse effect | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Gene expression | Restored | Quantitative pcr (qrt-pcr) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Cytoskeletal organization | Restored | Immunohistochemistry | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | Restored | Western blot | 5-8 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | Ameliorated | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | Side effect | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
| Molecular profile | Chromatin modification: histone modification: open chromatin | Refractory | Chromatin immunoprecipitation (chip) | 5-6 weeks | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_ROMIDEPSIN-2
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of high-dose romidepsin in mice bearing a heterozygous ablation of exon 21 (1mg/kg, i.p., 3 times).
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_ROMIDEPSIN-3
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of lower dose romidepsin in mice bearing a heterozygous ablation of exon 21 (0.025 mg/kg, i.p., 3 times). .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_TATCOFILIN-1
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Shank3-exon 21 deleted het mice were given an injection of Tat-p-cofilin at a dose of 15 picomol/g (i.v.), this is a Tat conjugated 16 mer peptide with Ser3 - phosphorylated consisted of the first 16 residues of cofilin. It is an inhibitor of endogenous cofilin found in increased activated form in the mutant mice. Injections were given before behavioral testing in juvenile male mice.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | Motor coordination and balance | No adverse effect | Accelerating rotarod test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Motor phenotype | General locomotor activity | No adverse effect | Open field test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Restored | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Sustained effect | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Sustained effect | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Restored | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | Restored | Grooming behavior assessments | 6-8 weeks | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Sustained effect | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | Anxiety | No adverse effect | Open field test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Sustained effect | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Sustained effect | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Restored | Western blot | 6-8 weeks | Duffney LJ , et al. (2015) |
| Molecular profile | Cytoskeletal organization | Restored | Immunostaining | 6-8 weeks | Duffney LJ , et al. (2015) |
M_SHANK3_8_KO_HT_TATCOFILIN-2
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Shank3-exon 21 deleted het mice got bilateral stereotaxic injections of the cofilin inhibitory peptide (dose not specified in article but likely 15 picomol/g, same as i.v. injection) into the prefrontal cortex prior to social behavior testing.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Restored | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic neuroreceptor ratio (nmdar/ampar) dependent transmission | Restored | Whole-cell patch clamp | NA | Duffney LJ , et al. (2015) |
| Repetitive behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Restored | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Molecular profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
M_SHANK3_8_KO_HT_TATCOFILIN-3
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Shank3-exon 21 del het mice were given an injection of Tat-p-cofilin at a low dose of 0.15 picomol/g (i.v.). This was done to examine the effects of lower dose of the Tat conjugated 16 mer Ser3 - phosphorylated cofilin peptide (inhibits endogenous cofilin). Injections were given before behavioral testing in juvenile male mice.
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6
Es cell line: -
Model Source: -
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Motor phenotype | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Neurophysiology | Synaptic transmission: excitatory | Refractory | Whole-cell patch clamp | 6-8 weeks | Duffney LJ , et al. (2015) |
| Repetitive behavior | Self grooming: perseveration | Refractory | Grooming behavior assessments | 6-8 weeks | Duffney LJ , et al. (2015) |
| Seizure | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Sensory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Social behavior | Social approach | Refractory | Three-chamber social approach test | 6-8 weeks | Duffney LJ , et al. (2015) |
| Physiological parameters | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Communications | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Developmental profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Emotion | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Immune response | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Learning & memory | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Maternal behavior | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
| Molecular profile | NA | Not reported | NA | NA | Duffney LJ , et al. (2015) |
M_SHANK3_8_KO_HT_TG003
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Shank3-exon 21 deleted het mice were treated by IP injection with 30mg/kg TG003, CLK2 inhibitor. Parent model was previously described in PMID 2425956 and has lost the majority of the Shank3 C-terminus; major isoforms are reported to be absent but some smaller isoforms are detected.
Allele Type: Knockout
Strain of Origin: C57BL/6J
Genetic Background: C57BL/6J
Es cell line: C57BL/6J ES cells
Model Source: Generated by the authors of PMID26847545
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Motor phenotype | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Neurophysiology | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Repetitive behavior | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Seizure | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Sensory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Social behavior | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Physiological parameters | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Communications | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Developmental profile | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Emotion | Exploratory activity | Refractory | Marble-burying test | Adult | Bidinosti M , et al. (2016) |
| Immune response | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Learning & memory | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Maternal behavior | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
| Molecular profile | NA | Not reported | NA | NA | Bidinosti M , et al. (2016) |
M_SHANK3_8_KO_HT_TSA
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of pan-HDAC inhibitor trichostatin A (once daily for 3 d) in mice bearing a heterozygous ablation of exon 21. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Ameliorated | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
M_SHANK3_8_KO_HT_VPA
Model Type: RESCUE-Pharmaceutical
Model Genotype: Heterozygous
Mutation:
Systemic administration of mood stabilizer valproic acid (once daily for 3 d), a low-affinity class I HDAC inhibitor in mice bearing a heterozygous ablation of exon 21. .
Allele Type: Knockout
Strain of Origin: 129S6/SvEvTac
Genetic Background: C57BL/6J
Es cell line: -
Model Source: PMID 24259569
Phenotypic Profile
| Category | Entity | Value Change | Experimental Paradigm | Age at Testing | Author, Year |
|---|---|---|---|---|---|
| Circadian sleep/wake cycle | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Motor phenotype | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neuroanatomy / ultrastructure / cytoarchitecture | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Neurophysiology | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Repetitive behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Seizure | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Sensory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Social behavior | Social approach | Ameliorated | Three-chamber social approach test | 5-8 weeks | Qin L , et al. (2018) |
| Physiological parameters | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Communications | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Developmental profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Emotion | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Immune response | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Learning & memory | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Maternal behavior | NA | Not reported | NA | NA | Qin L , et al. (2018) |
| Molecular profile | NA | Not reported | NA | NA | Qin L , et al. (2018) |
Other SHANK3 Rescue Models(46 Models)
| M_SHANK3_1_KO_HT | 2 | Genetic | Pharmaceutical | Insulin-like growth factor 1 |
| M_SHANK3_1_KO_HT | 1 | Genetic | Pharmaceutical | Insulin-like growth factor 1 peptide derivative |
| M_SHANK3_4_KO_HM | 1 | Genetic | Pharmaceutical | Cocaine |
| M_SHANK3_4_KO_HM | 1 | Genetic | Pharmaceutical | CX-546 |
| M_SHANK3_4_KO_HM | 2 | Genetic | Pharmaceutical | D-Cycloserine |
| M_SHANK3_4_KO_HM | 1 | Genetic | Pharmaceutical | 7,8-Dihydroxyflavone |
| M_SHANK3_4_KO_HM | 1 | Genetic | Dietary | Lithium |
| M_SHANK3_4_KO_HM | 1 | Genetic | Dietary | Lactobacillus reuteri |
| M_SHANK3_4_KO_HM | 1 | Genetic | Dietary | Lactobacillus reuteri |
| M_SHANK3_4_KO_HM | 1 | Genetic | Pharmaceutical | Oxytocin |
| M_SHANK3_4_KO_HM | 1 | Genetic | Dietary | Zinc |
| M_SHANK3_8_KO_HM | 1 | Genetic | Procedural | 6-hydroxydopamine |
| M_SHANK3_8_KO_HM | 1 | Genetic | Behavioral | Environmental enrichment |
| M_SHANK3_8_KO_HM | 1 | Genetic | Behavioral | Familiar feature |
| M_SHANK3_8_KO_HM | 1 | Genetic | Procedural | PreL-to-TS activation |
| M_SHANK3_8_KO_HM | 2 | Genetic | Pharmaceutical | SCH-23390 |
| M_SHANK3_8_KO_HM | 1 | Genetic | Pharmaceutical | TG003 |
| M_SHANK3_8_KO_HT | 4 | Genetic | Pharmaceutical | Romidepsin |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | Aripiprazole |
| M_SHANK3_8_KO_HT | 1 | Genetic | Genetic | constititutively active Rac1 |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | Clozapine |
| M_SHANK3_8_KO_HT | 1 | Genetic | Genetic | beta-catenin sh RNA |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | Fluoxetine |
| M_SHANK3_8_KO_HT | 2 | Genetic | Genetic | Hdac2 shRNA |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | Risperidone |
| M_SHANK3_8_KO_HT | 3 | Genetic | Pharmaceutical | Phospho-cofilin peptide |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | TG003 |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | Trichostatin A |
| M_SHANK3_8_KO_HT | 1 | Genetic | Pharmaceutical | Valproic acid |
| M_SHANK3_10_KI_HM_INSG | 1 | Genetic | Genetic | Reinstatement of Shank3 |
| M_SHANK3_21_KI_HM | 1 | Genetic | Genetic | Reinstatement of Shank3 |
| M_SHANK3_22_KI_HM | 2 | Genetic | Genetic | Reinstatement of Shank3 |
| M_SHANK3_19_KO_HM | 1 | Genetic | Pharmacological | 7-nitroindazole |
| M_SHANK3_19_KO_HM | 2 | Genetic | Pharmaceutical | CDPPB |
| M_SHANK3_19_KO_HM | 1 | Genetic | Pharmaceutical | MPEP |
| M_SHANK3_19_KO_HT | 1 | Genetic | Pharmaceutical | HC-067047 |
| M_SHANK3_25_KO_HM | 1 | Genetic | Procedural | ACC optogenetic activation |
| M_SHANK3_25_KO_HM | 1 | Genetic | Procedural | ACC chemogenetic activation |
| M_SHANK3_25_KO_HM | 4 | Genetic | Pharmaceutical | CX-546 |
| M_SHANK3_25_KO_HM | 1 | Genetic | Pharmaceutical | Lipopolysaccharide |
| M_SHANK3_25_KO_HM | 1 | Genetic | Procedural | Primary somatosensory cortex dysgranular zone (S1DZ) optogenetic inactivation |
| M_SHANK3_30_KO_HT | 3 | Genetic | Pharmaceutical | Isoguvacine |
| M_SHANK3_31_KI_HT | 3 | Genetic | Genetic | Reinstatement of Shank3 |
| M_SHANK3_32_KI_HM | 1 | Genetic | Genetic | Reinstatement of Shank3 |
| M_SHANK3_37_KD | 1 | Genetic | Genetic | CNO |
| M_SHANK3_37_KD | 1 | Genetic | Pharmaceutical | HC-067047 |
CNVs associated with SHANK3(1 Models)
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| 22q13.33 | 74 | Deletion-Duplication | 103 / 249 |