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Relevance to Autism

Several studies have identified rare mutations in the SHANK2 gene in autistic and intellectually disabled individuals (Berkel et al., 2010; Pinto et al., 2010; Leblond et al., 2012; Sanders et al., 2012). A functional study analyzed three of the mutations previously found in individuals with ASD (L1008_P1009dup, T1127M and R462X). The mutations were found to have varying effects on protein localization, dendritic spine volume and branching in cultured neurons and even synaptic transmission and cognitive behavior in mice, with the R462X variant causing the most severe phenotypes (Berkel et al., 2012).

Molecular Function

Shank proteins contain multiple domains for protein-protein interactions and function as molecular scaffolds in the postsynaptic density (PSD).

External Links

       

References

Type
Title
Type of Disorder
Associated Disorders
Author, Year
Primary
Mutations in the SHANK2 synaptic scaffolding gene in autism spectrum disorder and mental retardation.
ASD
MR
Negative association
Lack of association between NLGN3, NLGN4, SHANK2 and SHANK3 gene variants and autism spectrum disorder in a Chinese population.
ASD
Support
Functional impact of global rare copy number variation in autism spectrum disorders.
ASD
Support
Dysfunction of SHANK2 and CHRNA7 in a patient with intellectual disability and language impairment supports genetic epistasis of the two loci.
DD, ID
Support
Breakpoint mapping by next generation sequencing reveals causative gene disruption in patients carrying apparently balanced chromosome rearrangements with intellectual deficiency and/or congenital malformations.
DD, ID
Support
A discovery resource of rare copy number variations in individuals with autism spectrum disorder.
ASD
Support
Range of genetic mutations associated with severe non-syndromic sporadic intellectual disability: an exome sequencing study.
ID
Epilepsy, ASD
Support
De novo mutations revealed by whole-exome sequencing are strongly associated with autism.
ASD
Support
Genetic and functional analyses of SHANK2 mutations suggest a multiple hit model of autism spectrum disorders.
Autism
ID
Highly Cited
The interaction of phospholipase C-beta3 with Shank2 regulates mGluR-mediated calcium signal.
Highly Cited
The Shank family of scaffold proteins.
Recent Recommendation
Inherited and de novo SHANK2 variants associated with autism spectrum disorder impair neuronal morphogenesis and physiology.
ASD
ID
Recent Recommendation
Activity induced changes in the distribution of Shanks at hippocampal synapses.
Recent Recommendation
BetaPix up-regulates Na+/H+ exchanger 3 through a Shank2-mediated protein-protein interaction.
Recent Recommendation
AnkyrinG is required to maintain axo-dendritic polarity in vivo.

Rare

Variant ID
Variant Type
Allele Change
Residue Change
Inheritance Pattern
Inheritance Association
Family Type
Author, Year
 GEN229R001 
 Deletion 
 N/A 
 N/A 
 De novo 
  
  
 GEN229R002 
 Nonsense 
 C1384T 
 Arg462X 
 De novo 
  
  
 GEN229R003 
 Missense 
 C76T 
 Arg26Trp 
 Familial 
 Paternal 
  
 GEN229R004 
 Missense 
 C622T 
 Pro208Ser 
 Familial 
 Maternal 
  
 GEN229R005 
 Duplication 
 3024_3029dup 
 Leu1008_Pro1009dup 
 Familial 
 Maternal 
  
 GEN229R006 
 Missense 
 C3380T 
 Thr1127Met 
 Familial 
 Maternal 
  
 GEN229R007 
 Missense 
 G4048A 
 Ala1350Thr 
 Familial 
 Maternal 
  
 GEN229R008 
 Deletion 
 N/A 
 N/A 
 De novo 
  
  
 GEN229R009 
 Deletion 
 N/A 
 N/A 
 De novo 
  
  
 GEN229R010 
 Deletion 
 N/A 
 N/A 
 De novo 
  
  
 GEN229R011 
 Missense 
 G70344284A 
 R443C 
  
  
  
 GEN229R012 
 Missense 
 C70222501A 
 R598L 
  
  
  
 GEN229R013 
 Missense 
 C70026597A 
 V717F 
 Familial 
 Paternal 
 Simplex 
 GEN229R014 
 Missense 
 C70026561T 
 A729T 
 Familial 
 Maternal 
 Multiplex 
 GEN229R015 
 Missense 
 C70010562T 
 E1162K 
  
  
  
 GEN229R016 
 Missense 
 C70010538T 
 G1170R 
 Familial 
 Maternal 
 Simplex 
 GEN229R017 
 Missense 
 C70009920T 
 V1376I 
  
  
  
 GEN229R018 
 Missense 
 C70009443T 
 D1535N 
 Familial 
 Maternal 
 Simplex 
 GEN229R019 
 Missense 
 A69997007G 
 L1722P 
 Familial 
 Paternal 
 Simplex 
 GEN229R020 
 Missense 
 G70344381A 
 T410M 
  
  
  
 GEN229R021 
 Missense 
 C70322303T 
 S557N 
  
  
  
 GEN229R022 
 Missense 
 C69997021T 
 M1717I 
  
  
  
 GEN229R023 
 Missense 
 A467G 
 K156R 
  
  
  
 GEN229R024 
 Silent 
 C492T 
 G164G 
  
  
  
 GEN229R025 
 Substitution 
 G526+61A 
 N/A 
  
  
  
 GEN229R026 
 Substitution 
 C527-18A 
 N/A 
  
  
  
 GEN229R027 
 Substitution 
 G527-8T 
 N/A 
  
  
  
 GEN229R028 
 Missense 
 G569A 
 R190H 
  
  
  
 GEN229R029 
 Substitution 
 C640+11T 
 N/A 
  
  
  
 GEN229R030 
 Substitution 
 C800-33T 
 N/A 
  
  
  
 GEN229R031 
 Substitution 
 G924+19A 
 N/A 
  
  
  
 GEN229R032 
 Substitution 
 G924+133C 
 N/A 
  
  
  
 GEN229R033 
 Substitution 
 C1061-81T 
 N/A 
  
  
  
 GEN229R034 
 Substitution 
 G1141+49A 
 N/A 
  
  
  
 GEN229R035 
 Substitution 
 C1148-109T 
 N/A 
  
  
  
 GEN229R036 
 Missense 
 A1201C 
 K401Q 
  
  
  
 GEN229R037 
 Silent 
 G1284A 
 P428P 
  
  
  
 GEN229R038 
 Substitution 
 C302+14A 
 N/A 
  
  
  
 GEN229R039 
 Substitution 
 G1302+35A 
 N/A 
  
  
  
 GEN229R040 
 Substitution 
 C1303-54T 
 N/A 
  
  
  
 GEN229R041 
 Missense 
 G1316A 
 R439H 
  
  
  
 GEN229R042 
 Silent 
 G1392T 
 T464T 
  
  
  
 GEN229R043 
 Missense 
 A1763G 
 Y588C 
  
  
  
 GEN229R044 
 Silent 
 G1923A 
 E641E 
  
  
  
 GEN229R045 
 Silent 
 G2052A 
 P684P 
  
  
  
 GEN229R046 
 Silent 
 C2823T 
 D941D 
  
  
  
 GEN229R047 
 Silent 
 C3135T 
 P992P 
  
  
  
 GEN229R048 
 Silent 
 C3324T 
 A1108A 
  
  
  
 GEN229R049 
 Missense 
 C3620T 
 P1207L 
  
  
  
 GEN229R050 
 Substitution 
 T3843-12C 
 N/A 
  
  
  
 GEN229R051 
 Frameshift Insertion 
 2542insCA 
 N/A 
 De novo 
  
 Simplex 
 GEN229R052 
 Coding-synonymous 
 c.132G>A 
 No change 
 De novo 
  
 Simplex 
 GEN229R053 
 Deletion 
  
  
 Unknown 
  
 Unknown 
 GEN229R054 
 Inversion 
  
  
 De novo 
  
 Simplex 
 GEN229R055 
 Translocation 
  
  
 De novo 
  
  

Common

No Common Variants Available



Model Summary

Different abnormalities in synaptic glutamate receptor expression can cause alterations in social interactions and communication

External Links

AllenBrainAtlas   MGI Logo  Entrez Gene

References

Type
Title
Author, Year
Primary
Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2.
Additional
Autistic-like social behaviour in Shank2-mutant mice improved by restoring NMDA receptor function.

SHANK2_1_KO_HM

Model Type: Genetic
Model Genotype: Homozygous
Mutation: Homologous recombination mediated deletion of exon 7 of Shank2 gene
Allele Type: Targeted (Knock Out)
Strain of Origin: C57BL/6
Genetic Background: Not Specified
ES Cell Line: RI
Mutant ES Cell Line: Not specified
Model Source: Not specified

SHANK2_2_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous
Mutation: Homologous recombination mediated deletion of exon 7 of Shank2 gene
Allele Type: Targeted (Knock Out)
Strain of Origin: C57BL/6
Genetic Background: Not Specified
ES Cell Line: RI
Mutant ES Cell Line: Not specified
Model Source: Not specified

SHANK2_3_KO_HM

Model Type: Genetic
Model Genotype: Homozygous
Mutation: Targeted deletion of exons 6 and 7 and a frame shift of Shank2 gene
Allele Type: Targeted (Knock Out)
Strain of Origin: Not specified
Genetic Background: Not Specified
ES Cell Line: Not specified
Mutant ES Cell Line: Not specified
Model Source: Not specified

SHANK2_4_KO_HT

Model Type: Genetic
Model Genotype: Heterozygous
Mutation: Targeted deletion of exons 6 and 7 and a frame shift of Shank2 gene
Allele Type: Targeted (Knock Out)
Strain of Origin: Not specified
Genetic Background: Not Specified
ES Cell Line: Not specified
Mutant ES Cell Line: Not specified
Model Source: Not specified

SHANK2_1_KO_HM

Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Size/Weight1
Decreased
Description: Decreased body weight
Exp Paradigm: Female mice: General Observations
 General Observations
 2 weeks
Size/Weight1
Decreased
Description: Decreased body weight
Exp Paradigm: General Observations
 General Observations
 20-22 days; 4 months
Synaptic Transmission1
Decreased
Description: Decreased synaptic transmission demonstrated by decreased field excitatory postsynaptic potentials (fEPSPs)
Exp Paradigm: Extracellular field and whole-cell patch clamp recordings from CA1 pyramidal cells in acute hippocampal slices
 Extracellular field and whole-cell patch clamp recordings
 P21-P28; 3 months
Synaptic Transmission1
Decreased
Description: Decreased synaptic transmission indicated by reduction in miniature excitatory postsynaptic currents (mEPSC) frequency
Exp Paradigm: Whole-cell patch clamp recordings from CA1 pyramidal cells
 Whole-cell patch clamp recordings
 
NMDA/AMPA Ratio1
Increased
Description: Increased NMDA/AMPA ratio
Exp Paradigm: Whole-cell patch clamp recordings from CA1 pyramidal cells of compound EPSCs were evoked at -60 and +40 mV
 Whole-cell patch clamp recordings
 
Synaptic Plasticity1
Increased
Description: Increased synaptic plasticity indicated by enhanced long-term potentiation induced by hig h frequency stimulation of Schaffer collaterals
Exp Paradigm: Whole-cell patch clamp recordings of long-term potentiation induced by a single tetanus of 100 pulses at 100 Hz
 Whole-cell patch clamp recordings
 
Spine Density1
Decreased
Description: Decreased spine numbers in CA1 region of hippocampus
Exp Paradigm: Golgi-cox staining of CA1 hippocampal neurons
 Golgi-cox staining
 
NMDAR Subnit Levels1
Increased
Description: Increased NMDAR subunit levels in cortex, hippocampus and striatum
Exp Paradigm: emi-quantitative analysis of proteins in crude synaptosomal fractions from brain regions
 Semi-quantitative analysis of proteins
 P70
NMDAR Subnit Levels1
Increased
Description: Increased NMDAR subunit levels in hippocampus and striatum
Exp Paradigm: emi-quantitative analysis of proteins in crude synaptosomal fractions from hippocampus and striatum
 Semi-quantitative analysis of proteins
 P25
Ionotropic Glutamate Receptor content1
Increased
Description: Increased synaptic content of ionotropic glutamate receptor
 
 
NMDAR Subnit Levels1
Increased
Description: Increased levels of N-methyl-D- aspartate receptor (NMDAR) subunit GluN1 and ProSAP2/Shank3 in whole brain PSDs
Exp Paradigm: Immunoblot analysis of whole brain PSDs
 Immunoblot Analysis
 
Social Interaction1
Decreased
Description: Decreased social interactions indicated by difficulty maintaining social contacts
Exp Paradigm: Free same-sex social interactions (residentintruder),
 Resident intruder test
 
Social Novelty1
Decreased
Description: Decreased social novelty demonstrated by reduction in recognition and interest for social novelty
Exp Paradigm: Three chambered test
 Three chambered test
 
Social Interaction1
Decreased
Description: Decreased social interaction indicated by increased latency for first contact
Exp Paradigm: Free interactions of a tested male mouse with an oestrus C57BL/6 female mouse
 Free interaction
 
Ultrasonic Vocalizations1
Decreased
Description: Decreased ultrasonic vocalizations indicated by longer latency to emit first vocalization as well as short and unstructured calls during male-esterous female interactions
Exp Paradigm: Ultrasonic Vocalization (USV) during male-esterous female free interaction
 USV in socio-sexual context
 
Ultrasonic Vocalizations1
Decreased
Description: Decreased ultrasonic vocalizations indicated by longer latency to emit first vocalization, fewer vocalization plus short and unstructured calls during female-female interactions
Exp Paradigm: Ultrasonic Vocalization (USV) during female-female free interaction
 USV in same-sex free interaction
 
Ultrasonic Vocalizations1
Increased
Description: Increased ultrasonic vocalization call rate
Exp Paradigm: Female mice: Pup isolation calls
 Pup isolation calls
 P4, P10
Digging1
Decreased
Description: Decreased digging behavior
 
 
Self Grooming1
Increased
Description: Increased self grooming behavior
Exp Paradigm: Female mice: Observation of self grooming behavior
 
 
Righting Response1
Increased
Description: Increased righting response as measured by decreased latency to right up
Exp Paradigm: Male mice: Righting reflex
 Righting reflex
 P4, P10
General Locomotor Activity1
Increased
Description: Increased locomotor activity as measured by distance traveled and number of transitions
Exp Paradigm: Open Field Test, 3 chamber test, Y maze test
 Open Field Test
 
Limb Posture1
Abnormal
Description: Abnormal limb posture indicated by hindlimb clasping
Exp Paradigm: Tail hanging assay
 Tail hanging assay
 4 months
Righting Response1
Decreased
Description: Decreased righting response as measured by increased latency to right up
Exp Paradigm: Female mice: Righting reflex
 Righting reflex
 P8
Ultrasonic Vocalizations1
 No change
 Pup isolation calls
 P4, P10
Ultrasonic Vocalizations1
 No change
 USV in same-sex free interaction
 
Size/Weight1
 No change
 General Observations
 2 weeks
Object Recognition Memory1
 No change
 Object recognition test
 
Spatial Working Memory1
 No change
 Y-maze test
 
Shank3 expression1
 No change
 Immunoblot; RT-PCR
 
Motor coordination1
 No change
 Rotarod test
 6-8 months
Synaptic Ultrastructure1
 No change
 Electron microscopic analysis
 
Long Term Depression1
 No change
 Whole-cell patch clamp recordings
 
Synaptic Function1
 No change
 
 
Synaptic Transmission1
 No change
 Whole-cell patch clamp recordings
 
Synaptic Transmission1
 No change
 Whole-cell patch clamp recordings
 
Olfaction1
 No change
 Buried food finding test
 
Olfaction1
 No change
 Odor preference test
 7 days
 Not Reported: circadian sleep/wake cycle ,   Homeostasis ,   Inflammatory response ,   Maternal behavior ,   Seizure ,  

SHANK2_2_KO_HT

Category
Entity
Quantity
Experimental Paradigm
Age at Testing
NMDAR Subnit Levels1
Increased
Description: Increased NMDAR subunit levels in hippocampus and striatum
Exp Paradigm: emi-quantitative analysis of proteins in crude synaptosomal fractions from hippocampus and striatum
 Semi-quantitative analysis of proteins
 P25
Object Recognition Memory1
 No change
 Object recognition test
 
Spatial Working Memory1
 No change
 Y-maze test
 
Righting Response1
 No change
 Righting reflex
 P4, P8, P10
Olfaction1
 No change
 Buried food finding test
 
Olfaction1
 No change
 Odor preference test
 7 days
 Not Reported: circadian sleep/wake cycle ,   Communications ,   Developmental profile ,   Emotion ,   Homeostasis ,   Inflammatory response ,   Maternal behavior ,   Neuroanatomy / Ultrastructure / Cytoarchitecture ,   Neurophysiology ,   Repetitive behavior ,   Seizure ,   Social behavior ,  

SHANK2_3_KO_HM

Category
Entity
Quantity
Experimental Paradigm
Age at Testing
Long Term Depression1
Decreased
Description: Decreased long-term depression (LTD) induced by low-frequency stimulation at SC-CA1 synapses
Exp Paradigm: Extracellular recordings at hippocampal Schaffer-collateral-CA1-pyramidal synapses of LTD induced by DHPG
 Extracellular recordings
 5-6 weeks
NMDA/AMPA Ratio1
Decreased
Description: Decreased NMDA/AMPA ratio at SC-CA1 synapses
Exp Paradigm: Extracellular recordings of NMDAR and AMPAR transmission
 Extracellular recordings
 3-4 weeks
Synaptic Plasticity1
Decreased
Description: Decreased synaptic plasticity indicative of impaired long-term potentiation at SC-CA1 synapses
Exp Paradigm: Extracellular recordings at hippocampal Schaffer-collateral-CA1-pyramidal synapses of LTP induced by theta-burst stimulation
 Extracellular recordings
 8-9 weeks
NMDAR associated signaling1
Decreased
Description: Decreased NMDAR associated signaling indicated by lower levels of phosphorylated CaMKII-alpha/beta, ERK1/2 , p38, GluA1 (S831 and S845)
Exp Paradigm: Immunoblot analysis of whole brain homogenates for makers of NMDAR associated signaling
 Immunoblot Analysis
 3-4 weeks
Shank2 mRNA levels1
Decreased
Description: Decreased Shank2 mRNA levels in brain
Exp Paradigm: RT-PCR Analysis of brain tissue
 RT-PCR Analysis
 
Shank2 protein expression1
Decreased
Description: Decreased Shank2 protein expression in synaptic fractions
Exp Paradigm: Immunoblot analysis of synaptosomes
 Immunoblot Analysis
 2-3 months
Nest building behavior1
Decreased
Description: Decreased nest building behavior
Exp Paradigm: Nest building assay
 Nest building assay
 2-5 months
Social Interaction1
Decreased
Description: Decreased social interaction demonstrated by decreased preference to explore novel mouse over inanimate object
Exp Paradigm: Three-chambered social interaction assay between novel mouse and inanimate object
 Three-chambered test
 4-5 weeks
Social Interaction1
Decreased
Description: Decreased social interaction with normal target mice
Exp Paradigm: Home-cage social interaction assay
 Social interaction assay
 2-5 months
Ultrasonic Vocalizations1
Decreased
Description: Decreased ultrasonic vocalizations as indicated by longer latency and lower frequency of calls
Exp Paradigm: Ultrasonic Vocalization (USV) during interaction of male with novel female
 USV in a social context
 2-5 months
Self Grooming1
Increased
Description: Increased self grooming behavior
Exp Paradigm: Observation of self grooming during object recognition test
 Observation during object recognition test
 2-5 months
Digging1
Decreased
Description: Decreased digging behavior
Exp Paradigm: Observation of repetitive behavior in home cage
 Stereotypical behavior observations
 2-5 months
Jumping1
Increased
Description: Increased repetitive jumping behavior mostly mixed with upright scrabbling
Exp Paradigm: Observation of repetitive behavior in home cage
 Observation of repetitive behavior
 2-5 months
Pup Retrieval1
Decreased
Description: Decreased pup retrieval efficiency of female mice
Exp Paradigm: Pup retrieval assay
 Pup retrieval assay
 2-5 months
General Locomotor Activity1
Increased
Description: Increased locomotor activity indicative of hyperactivity measured as the distance traveled
Exp Paradigm: Open Field Test; Elevated plus maze test
 Open Field Test; Elevated plus maze test
 2-5 months
Spatial Learning1
Decreased
Description: Decreased spatial learning as indicated by reduced amount of time in target quadrant and less frequency of crossing platform area
Exp Paradigm: Morris water maze
 Morris water maze
 2-5 months
Reproductive Structure1
 No change
 
 
Anxiety1
 No change
 Open Field Test; Light/Dark Box
 2-5 months
Object Recognition Memory1
 No change
 Novel object recognition assay
 2-5 months
NMDAR associated signaling1
 No change
 Immunoblot Analysis
 3-4 weeks
Shank1/3 protein expression1
 No change
 Immunoblot Analysis
 3-4 weeks
Brain cytoarchitecture1
 No change
 Electron microscopic analysis
 8-9 weeks
Brain cytoarchitecture1
 No change
 Immunofluorescence staining
 3 months
Brain cytonumber1
 No change
 
 
Brain Morphology1
 No change
 Immunostaining analysis
 
Basal synaptic transmission1
 No change
 Extracellular recordings
 8-9 weeks
NMDA/AMPA Ratio1
 No change
 Extracellular recordings
 3-4 weeks
Olfaction1
 No change
 Sniffing behavior towards target
 2-5 months
Social Novelty1
 No change
 Three-chambered test
 4-5 weeks
 Not Reported: circadian sleep/wake cycle ,   Homeostasis ,   Inflammatory response ,   Seizure ,  

SHANK2_4_KO_HT

Category
Entity
Quantity
Experimental Paradigm
Age at Testing
General Locomotor Activity1
Increased
Description: Increased locomotor activity indicative of hyperactivity
Exp Paradigm: Open Field Test; Elevated plus maze test
 Open Field Test; Elevated plus maze test
 2-5 months
Anxiety1
 No change
 Elevated plus maze test
 2-5 months
Object Recognition Memory1
 No change
 Novel object recognition assay
 2-5 months
Digging1
 No change
 Stereotypical behavior observations
 2-5 months
Jumping1
 No change
 Observation of repetitive behavior
 2-5 months
Self Grooming1
 No change
 Observation during object recognition test
 2-5 months
Social Interaction1
 No change
 Three-chambered test
 4-5 weeks
 Not Reported: circadian sleep/wake cycle ,   Communications ,   Developmental profile ,   Homeostasis ,   Inflammatory response ,   Maternal behavior ,   Molecular profile ,   Neuroanatomy / Ultrastructure / Cytoarchitecture ,   Neurophysiology ,   Seizure ,   Sensory ,  



Interactor Symbol Interactor Name Interactor Organism Entrez ID Uniprot ID Interaction Type Evidence Reference
DNM2 dynamin 2 1785 P50570 GST (purified proteins)
IP/WB (COS-7 cells)
IP/WB (rat brain)
Y2H
Okamoto PM 2001
PDE4D phosphodiesterase 4D, cAMP-specific 5144 Q08499 GST (purified protein and CHO cell lysate)
IP/WB (CHO cells)
IP/WB (COS-7 cells)
IP/WB (mouse brain)
IP/WB (T84 cells)
Lee JH 2007
FMR1 fragile X mental retardation 1 14265 P35922 Darnell JC 2011
GRID2 glutamate receptor, ionotropic, delta 2 14804 Q61625 GST (purified protein and COS-7 cell lysate)
IP/WB (COS-7 cells)
IP/WB (mouse brain)
surface plasmon resonance
Y2H
Uemura T 2004
GRIP1 glutamate receptor interacting protein 1 74053 Q925T6 IP/WB (mouse brain)
Uemura T 2004
ARHGEF7 Rho guanine nucleotide exchange factor (GEF7) 114559 O55043 GST (HEK293T cells)
IP/WB (HEK293T cells)
IP/WB (rat brain)
Y2H
Park E 2003
CFTR cystic fibrosis transmembrane conductance regulator homolog 12638 P26361 IP/WB (NIH-3T3 cells)
Y2H
Kim JY 2004
CTTN cortactin 60465 Q66HL2 GST (purified protein and NIH-3T3 cell lysate)
IP/WB (PC12 cells)
Y2H
Du Y 1998
DBNL Drebrin-like 13169 Q62418 Far Western Blot (FWB)
GST (rat brain)
Qualmann B 2004
DLG4 discs, large homolog 4 (Drosophila) 29495 P31016 IP/WB (rat brain)
Y2H
Boeckers TM 1999
DLGAP1 discs, large (Drosophila) homolog-associated protein 1 65040 P97836 GST (rat brain)
IP/WB (COS-7 cells)
IP/WB (rat brain)
Y2H
Naisbitt S 1999
DLGAP2 ?discs, large (Drosophila) homolog-associated protein 2 116681 P97837 Y2H
Boeckers TM 1999
DLGAP3 discs, large (Drosophila) homolog-associated protein 3 286923 P97838 Y2H
Boeckers TM 1999
DLGAP4 discs, large homolog-associated protein 4 (Drosophila) 286930 P97839 Y2H
Boeckers TM 1999
DYNLL1 dynein light chain LC8-type 1 58945 P63170 IP/WB (rat brain)
Naisbitt S 2000
DYNLL2 dynein light chain LC8-type 2 140734 Q78P75 IP/WB (rat brain)
Naisbitt S 2000
HOMER1 homer homolog 1 (Drosophila) 29456 Q9Z214 GST (purified protein and COS-7 cell lysate)
IP/WB (rat brain)
Hwang JI 2005
ITPR3 inositol 1,4,5-triphosphate receptor, type 3 25679 Q63269 IP/WB (rat brain)
Hwang JI 2005
LPHN1 latrophilin 1 65096 O88917 Far Western Blot (FWB)
IP/WB (rat brain)
Y2H
Kreienkamp HJ 2000
LRRC7 leucine rich repeat containing 7 117284 P70587 GST (purified proteins)
IP/WB (HEK293 cells)
IP/WB (rat brain)
Quitsch A 2005
MYO5A myosin VA 25017 Q9QYF3 IP/WB (rat brain)
Naisbitt S 2000
PLCB3 phospholipase C, beta 3 29322 Q99JE6 GST (purified protein and COS-7 cell lysate)
IP/WB (COS-7 cells)
IP/WB (rat brain)
Y2H
Hwang JI 2005
SLC4A7 solute carrier family 4, sodium bicarbonate cotransporter, member 7 117955 Q9R1N3 Y2H
Kim JY 2004
SLC9A3 solute carrier family 9 (sodium/hydrogen exchanger), member 3 24784 P26433 IP/WB (PS120 cells)
surface plasmon resonance
Y2H
Han W 2006
SSTR2 somatostatin receptor 2 54305 P30680 Far Western Blot (FWB)
IP/WB (HEK293 cells)
Y2H
Zitzer H 1999
LZTS2 leucine zipper, putative tumor suppressor 2 495421 Q5U4W1 IP/WB (Xenopus oocytes)
Gessert S 2011

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